Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 9,135 results for: sgRNA

Clear keywords
Showing: 7721 - 7740 of 9135 results

  4. pDONR221 SNAT2-eGFP 3NQ

    Plasmid
    #237232
    Purpose
    Donor vector for gateway cloning of sgRNA-resistant SNAT2 (SLC38A2) cDNA sequence containing C-terminal eGFP. SNAT2 sequence contains mutations to inhibit N-linked glycosylation.
    Depositor
    Seth Parker
    Article
    Koe et al Am J Physiol Cell Physiol. 2026 Jun 29.
    Insert
    SLC38A2 (SLC38A2 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Silent mutations to prevent targeting by sgRNA se…
    Available Since
    July 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    237232-plasmid-map-sequence-id-471117

    Enlarge

  5. pLenti CMV SNAT2-eGFP 3NQ

    Plasmid
    #237227
    Purpose
    Lentiviral destination vector expressing sgRNA-resistant SNAT2 (SLC38A2) cDNA containing C-terminal eGFP. SNAT2 sequence contains mutations to inhibit N-linked glycosylation.
    Depositor
    Seth Parker
    Article
    Koe et al Am J Physiol Cell Physiol. 2026 Jun 29.
    Insert
    SLC38A2 (SLC38A2 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Silent mutations to prevent targeting by sgRNA se…
    Promoter
    CMV
    Available Since
    July 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    237227-plasmid-map-sequence-id-473960

    Enlarge

  6. pInducer20 SNAT2-eGFP 3NQ

    Plasmid
    #237229
    Purpose
    Lentiviral vector expressing dox-inducible sgRNA-resistant SNAT2 (SLC38A2) cDNA containing C-terminal eGFP. SNAT2 sequence contains mutations to inhibit N-linked glycosylation.
    Depositor
    Seth Parker
    Article
    Koe et al Am J Physiol Cell Physiol. 2026 Jun 29.
    Insert
    SLC38A2 (SLC38A2 Human)
    Use
    Lentiviral
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    Silent mutations to prevent targeting by sgRNA se…
    Promoter
    TRE
    Available Since
    July 1, 2026
    Availability
    Academic Institutions and Nonprofits only
    237229-plasmid-map-sequence-id-473956

    Enlarge

  9. PB-2G-CNCB

    Plasmid
    #236764
    Purpose
    A piggybac-based cloning vector containing dual sgRNAs for spCas9 and CAG promoter-driven nuclear-localized Clover-T2A-BSR.
    Depositor
    Kazutoshi Takahashi
    Article
    Okubo et al Sci Adv. 2025 Apr 11;11(15):eadq5484. do
    Type
    Empty backbone
    Use
    Piggybac
    Expression
    Mammalian
    Promoter
    mouse U6 promoter, human U6 promoter, CAG promoter
    Available Since
    May 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    236764-plasmid-map-sequence-id-473439

    Enlarge

  10. pYPQAT02

    Plasmid
    #223374
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223374-plasmid-map-sequence-id-452305

    Enlarge

  11. qTAG-C-Solo-mScarlet-MAPRE1

    Plasmid
    #227323
    Purpose
    Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227323-plasmid-map-sequence-id-451725

    Enlarge

  12. qTAG-KO-Puro-TP53

    Plasmid
    #227319
    Purpose
    Donor template for 2A-Puro insertion into the N-terminus of the TP53 locus. For selectable p53 knock-out. To be co-transfected with sgRNA plasmid px330-TP53 (Addgene #227318)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TP53 Homology Arms flanking a 2A-Puro Cassette (TP53 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227319-plasmid-map-sequence-id-451734

    Enlarge

  13. qTAG-C-mStayGold-EFS-Puro-PLK4

    Plasmid
    #227311
    Purpose
    Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227311-plasmid-map-sequence-id-452079

    Enlarge

  14. qTAG-C-Solo-mStayGold-CEP192

    Plasmid
    #227289
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227289-plasmid-map-sequence-id-452061

    Enlarge

  15. pJMP8921

    Plasmid
    #220903
    Purpose
    MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (BsaI site), and PC-dCas9-novo
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220903-plasmid-map-sequence-id-437058

    Enlarge

  16. pJMP8932

    Plasmid
    #220906
    Purpose
    MobileCRiSPRi system encoded on a kanR Tn7 transposon; PD-sgRNA (BsaI site) and PG-dCas9-novo (for cloning new guides)
    Depositor
    Jason Peters
    Article
    Hall et al Appl Environ Microbiol. 2024 Sep 26:e003
    Insert
    dCas9-novo
    Use
    CRISPR
    Tags
    3xMyc
    Expression
    Bacterial
    Mutation
    D10A, H840A
    Available Since
    Sept. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220906-plasmid-map-sequence-id-436983

    Enlarge

Showing: 7721 - 7740 of 9135 results