We narrowed to 4,802 results for: pcr
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Plasmid#26886DepositorInsertHBH tag N-terminal
UsePcr tagging yeastAvailable SinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-PGAL1-3HA
Plasmid#41609DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTags3xHA, TRP1 reverse orientation, and yeast ADH1 te…Available SinceMay 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-PGAL1-GST
Plasmid#41611DepositorInsertGAL1 promoter (GAL1 Budding Yeast)
UseYeast genomic targetingTagsA. gossypii translation elongation factor 1a gene…Available SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GST-TRP1
Plasmid#41603DepositorAvailable SinceDec. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCR175
Plasmid#240640PurposeTemplate for in vitro transcription of mTurquoise2 mRNA using SP6 polymerase.DepositorInsertmTurquoise2
UseVertebrate gene expression and general in vitro t…PromoterSP6Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR178
Plasmid#240641PurposeTemplate for in vitro transcription of mScarlet-I mRNA using SP6 polymerase.DepositorInsertmScarlet-I
UseVertebrate gene expression and general in vitro t…PromoterSP6Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR182
Plasmid#240642PurposeTemplate for in vitro transcription of AmNeonGreen mRNA using SP6 polymerase.DepositorInsertAmNeonGreen
UseVertebrate gene expression and general in vitro t…MutationCodon-optimized for Exaiptasia diaphana, amino ac…PromoterSP6Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR197
Plasmid#240643PurposeExpression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymeraseDepositorInsertsAIPGENE865 promoter
SP6 promoter
AmNeonGreen
mTurquoise2
mScarlet-I
UseGene expression and genomic integration in fishTagsNES (from Exaiptasia diaphana MEK2), NLS (from SV…MutationCodon-optimized for Exaiptasia diaphana, amino ac…Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR241
Plasmid#240644PurposeExpression of AmNeonGreen-13myc in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymeraseDepositorInsertsAIPGENE865 promoter
SP6 promoter
AmNeonGreen
UseGene expression and genomic integration in fishTags13mycMutationCodon-optimized for Exaiptasia diaphana, amino ac…Available SinceAug. 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCR158
Plasmid#240639PurposeExpression of NLS-Kaede in Exaiptasia diaphanaDepositorInsertsAIPGENE865 promoter
Kaede
UseExpression of nls-kaede in exaiptasia diaphanaTagsSV40 NLSMutationnaturally occurring 749-bp insertion at bp 628 (f…PromoterExaiptasia diaphana Elongation Factor 1 alpha (AI…Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRSF_PcRbcLS
Plasmid#229519PurposeExpresses Phaeoceros carolinianus Rubisco large and small subunit (C terminus hexahistidine)DepositorInsertsRubisco large subunit
Rubisco small subunit
TagshexahistidineExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IHFdist_rm
Plasmid#162318PurposeP. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removedDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationIHF distal site is removedAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_IRdist_scram
Plasmid#162319PurposeP. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motifDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialMutationThe distal Upstream Motif site is scrambledPromoterpBADAvailable SinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC2
Plasmid#191857PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC6
Plasmid#191861PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC5
Plasmid#191860PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC4
Plasmid#191859PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC3
Plasmid#191858PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR3_BC1
Plasmid#191856PurposeBarcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.DepositorTypeEmpty backboneUseCRISPRAvailable SinceDec. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR2_wt
Plasmid#149386PurposeP. aeruginosa PA14 CRISPR2 locusDepositorInsertPseudomonas aeruginosa PA14 CRISPR2 locus
ExpressionBacterialPromoterpBADAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only