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plentiCRISPR-v2 Puro KIAA0141/DELE1_sg1

Plasmid

#218524

Purpose

sgRNA targeting human KIAA0141/DELE1

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

DELE1 (DELE1 Human)

Use

CRISPR and Lentiviral

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_PA4114_Cterm_His

Plasmid

#220484

Purpose

heterologous expression of PA4114 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

PA4114

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Cterm_His

Plasmid

#220487

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_Human_SAT1_Nterm_His

Plasmid

#220482

Purpose

heterologous expression of SSAT1 for protein purification

Depositor

Emily Balskus

Publication

Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

Insert

SSAT1 (SAT1 Human)

Tags

6xHs

Expression

Bacterial

Mutation

none

Available since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC236

Plasmid

#216368

Purpose

xhNup155-Nb2i (+2Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb2i (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC235

Plasmid

#216372

Purpose

xhNup155-Nb3i (+2Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup155-Nb3i (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP728

Plasmid

#216374

Purpose

xNup358-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup358-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC115

Plasmid

#216356

Purpose

xNup93-Nb2t Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup93-Nb2t

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pTP443

Plasmid

#216361

Purpose

xNup98-Nb1t (+1Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xNup98-Nb1t (+1Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC130

Plasmid

#216364

Purpose

xhNup98-Nb3i Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup98-Nb3i

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMSC227

Plasmid

#216351

Purpose

xhNup35-Nb1t (+3Cys) Nanobody production

Depositor

Dirk Görlich

Publication

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup35-Nb1t (+3Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

Plasmid

#218534

Purpose

human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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plentiCRISPR-v2 Puro KIAA0141/DELE1_sg2

Plasmid

#218525

Purpose

sgRNA targeting human KIAA0141/DELE1

Depositor

Kivanc Birsoy

Publication

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

DELE1 (DELE1 Human)

Use

CRISPR and Lentiviral

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL851

Plasmid

#214251

Purpose

Expression TadCBEd in rice cells

Depositor

Yong Zhang

Publication

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pGEL855

Plasmid

#214255

Purpose

Expression Td-CBEmax in rice cells

Depositor

Yong Zhang

Publication

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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M2e-noro-VLP

Plasmid

#201192

Purpose

Expresses noro-VLP decorated with influenza M2e in insect cells

Depositor

Vesa Hytönen

Publication

Lampinen et al Front Cell Infect Microbiol. 2023 Jun 22

Insert

M2e-noro-VLP

Tags

Influenza M2e peptide

Expression

Insect

Promoter

Polyhedrin

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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ACVR1-TAAS-R206H

Plasmid

#206184

Purpose

in vitro transcription of RNA encoding human ACVR1 with mutations S190A, S192A, and R206H

Depositor

Mary Mullins

Publication

Allen et al J Bone Miner Res. 2023 Jun 17. doi: 10.1

Insert

activin A receptor type 1 (ACVR1 Human)

Use

In vitro transcription

Tags

FLAG tag

Mutation

S190A, S192A, R206H

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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psicheck2-PHLPP2mut

Plasmid

#203606

Purpose

Reporter plasmid carrying PHLPP2 3'UTR with mutated miR-27b target sequences

Depositor

Hiroyuki Mizuguchi

Publication

Sakai et al Commun Biol. 2023 Jun 24;6(1):669. doi:

Insert

PH domain and leucine rich repeat protein phosphatase 2 (PHLPP2 Human)

Use

Luciferase

Expression

Mammalian

Mutation

miR-27b target sequences in the 3'UTR were m…

Promoter

SV40

Available since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Publication

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAJT254-litR

Plasmid

#202308

Purpose

litR repressor plasmid for LPattB2 integration

Depositor

Christopher Voigt

Publication

Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

Insert

litR

Expression

Bacterial

Available since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

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