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pIRES2-EGFP-p53 WT

Plasmid

#49242

Purpose

expresses human wt p53 and EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53 (TP53 Human)

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K33

Plasmid

#17607

Purpose

Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K33 only, other lysines mutated to arginines; P19…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244089

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pF155 pcDNA3.1(+)SOD1G93A

Plasmid

#26401

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G93A

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pF151 pcDNA3.1(+)SOD1WT

Plasmid

#26397

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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P021-mPB-ENGRAM-All-in-one-PGK-BFP-P2A-Blast

Plasmid

#239972

Purpose

Updated ENGRAM plasmid that is ready for both 5' and 3'-FT ENGRAM cloning

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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ABE8.8-m

Plasmid

#136294

Purpose

expresses ABE8.8-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.8-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-T2TP

Plasmid

#114725

Purpose

expression in mammalian cells

Depositor

Martine Roussel

Article

Kawauchi et al Oncogene. 2017 Sep 14;36(37):5231-5242.

Insert

Tol2 transposase

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLJC5-3xHA-eGFP-OMP25

Plasmid

#239249

Purpose

3xHA-eGFP-OMP25 lentiviral overexpression vector

Depositor

Jason Cantor

Article

Huggler et al Nat Metab. 2026 Jan 16. doi: 10.1038/s42

Insert

3xHA-eGFP-OMP25 (SYNJ2BP Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Promoter

Ubc

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pLV-ATF5-PROX1-HNF1A

Plasmid

#149725

Purpose

expressing three liver promoting transcription factors (ATF5, PROX1, HNF1A)

Depositor

Baisong Lu

Article

Lu et al Biomaterials. 2021 Feb;269:120668. doi:

Insert

ATF5-PROX1-HNF1A (ATF5 Human)

Use

Lentiviral

Tags

P2A, T2A

Promoter

CMV

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH1

Plasmid

#108699

Purpose

For expression of C-terminally eGFP-tagged human RNASEH1 (nuclear isoform) in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH1 (nuclear isoform) (RNASEH1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K48R

Plasmid

#17604

Purpose

Mammalian expression of HA tagged ubiquitin K48R

Depositor

Ted Dawson

Article

Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

Insert

Ubiquitin C (UBC Human)

Tags

HA

Expression

Mammalian

Mutation

K48R (expected to disrupt G76-K48 ubiquitin chain…

Available Since

March 31, 2008

Availability

Academic Institutions and Nonprofits only

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P022-mPB-TRE-PEmax-T2A-mCherry-PGK-rttA-Puro

Plasmid

#239974

Purpose

PiggyBac plasmid for inducible PEmax expression and Puromycin selection

Depositor

Jay Shendure

Article

Chen et al Nature. 2024 Aug;632(8027):1073-1081. do

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

TRE

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3

Plasmid

#228866

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMTTH+mEGFP-H1.4

Plasmid

#157799

Purpose

Expression of mEGFP and human linker histone H1.4 fusion protein

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

mEGFP-H1.4 (H1-4 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_miniENVLPE

Plasmid

#232431

Purpose

Expression plasmid to produce miniENVLPE particles

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

miniENVLPE

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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Intron-Tagging-mScarlet-Frame1-Parental-Minicircle

Plasmid

#216125

Purpose

Parental minicircle containing mScarlet-I flanked by a splice acceptor and splice donor. Used with other intron tagging plasmids for placing the mScarlet tag as a synthetic exon into frame 1 introns of target genes.

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

sgRNA-SA-(GGGGS)x4-mScarlet-I-(GGGGS)x4-SD

Use

CRISPR

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP

Plasmid

#193357

Purpose

Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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STIM1-2Strep

Plasmid

#210361

Purpose

Expresses STIM1 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

STIM1 (STIM1 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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OPA1-isoform1

Plasmid

#70173

Purpose

Mammalian expression of hsOPA1 isoform 1

Depositor

David Chan

Article

Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

Insert

hsOPA1 isoform 1 (OPA1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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