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GFP-Miro1 S156A

Plasmid

#127588

Purpose

Phospho-mutant of S156

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S156A

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_NAD-Snifit

Plasmid

#113658

Purpose

Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Bacterial

Promoter

T7

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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LoxP2-Mb2Tomato-2A (SO240)

Plasmid

#99614

Purpose

To clone gene of interest downstream of LoxP2-Mb2Tomato-2A cassette

Depositor

Rui Benedito

Article

Pontes-Quero et al Cell. 2017 Aug 10;170(4):800-814.e18. do

Insert

Mb2Tomato

Tags

T2A

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB135

Plasmid

#68589

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB328

Plasmid

#68656

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pX330(puro) Rosa26-H3F3B

Plasmid

#73131

Purpose

Expression in Mammalian Cells, Cas9 Nuclease to analize translocations

Depositor

Agnel Sfeir

Article

Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

Insert

spCas9

Tags

Flag

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pIW1

Plasmid

#52530

Purpose

serves as PCR-template to generate HR donors for C-terminal Twin-Strep tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Twin-STREP-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pFBXC3GH

Plasmid

#49051

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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MLM3583

Plasmid

#43887

Purpose

Activation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NG (binds T)

Depositor

Keith Joung

Article

Maeder et al Nat Methods. 2013 Feb 10. doi: 10.1038/n

Insert

TALE-activator VP64 Backbone

Use

Tale transcriptional activator expression vector

Promoter

EF1alpha

Available Since

March 8, 2013

Availability

Academic Institutions and Nonprofits only

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HOXC8-pSG5

Plasmid

#21001

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC8 (HOXC8 Human)

Expression

Mammalian

Mutation

missing first 24 N-terminal amino acids of coding…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
V309 - pDNR MCS SA lacZ alpha - Donor

Plasmid

#11692

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

Type

Empty backbone

Use

Cre/Lox; Donor vector

Available Since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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lvl0 G7t

Plasmid

#153380

Purpose

Agrobacterium terminator for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G7 terminator

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PHB1-mCherry

Plasmid

#232784

Purpose

Encodes an mCherry-tagged Prohibitin1/PHB1

Depositor

Giulia Bertolin

Article

Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

Insert

PHB1 (PHB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pCHW101020

Plasmid

#222454

Purpose

Level 1, Position 2 containing 35S::CUP2-p65::NOS.

Depositor

Chih-Hang Wu

Article

Chiang et al New Phytol. 2024 Oct;244(1):318-331. doi

Insert

35S::CUP2-p65::NOS (CUP2 )

Use

Synthetic Biology

Expression

Plant

Mutation

no

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSNRW-Z5-Nluc

Plasmid

#174487

Purpose

Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

Depositor

Richard C. Willson

Article

Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

Insert

Z5-Nluc

Tags

6x Histidine tag (N terminal on insert)

Expression

Bacterial

Promoter

T7lac promoter

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pJL057

Plasmid

#177169

Purpose

Expresses a nuclease dead MRE11 in human cells

Depositor

Brittany Adamson

Article

Hussmann et al Cell. 2021 Oct 28;184(22):5653-5669.e25.

Insert

MRE11 H129N (MRE11 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

nuclease-inactivating mutation (H129N)

Promoter

EF1a

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-BE4max-BlastR

Plasmid

#152991

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgNUAK1

Plasmid

#138692

Purpose

Expresses a human NUAK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgNUAK1 human (NUAK1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pESC/mCitrine

Plasmid

#117996

Purpose

Expression of mCitrine controlled by the Gal1 promoter

Depositor

Mathias Pribil

Article

Behrendorff et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

mCitrine

Use

Yeast/bacteria binary vector

Expression

Yeast

Promoter

Gal1

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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