Mobius Assembly for Plant Systems highlights promoter-terminator interaction in gene regulation
Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , Nakayama, Naomi
bioRxiv, March 31, 2021 Article
Andreou , Andreas I., Nirkko, Jessica , Ochoa-Villarreal, Marisol , Nakayama, Naomi
bioRxiv, March 31, 2021 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 153343 | pMAP L1A | Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication |
| 153344 | pMAP L1B | Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication |
| 153345 | pMAP L1Γ | Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication |
| 153347 | pLXMA L1A | Expression vector for plants with medium-copy number origin of replication |
| 153348 | pLXMA _RK2 L1A | Expression vector for plants with low-copy number origin of replication |
| 153349 | Auxiliary 1 | Provides Middle-to-End linker 1 for level 2 cloning |
| 153350 | Auxiliary 2 | Provides Middle-to-End linker 2 for level 2 cloning |
| 153351 | Auxiliary 3 | Provides Middle-to-End linker 3 for level 2 cloning |
| 153352 | Auxiliary A | Provides End-to-End linker 4A for level 2 cloning |
| 153353 | Auxiliary B | Provides End-to-End linker 4B for level 2 cloning |
| 153354 | Auxiliary Γ | Provides End-to-End linker 4Γ for level 2 cloning |
| 153355 | Auxiliary Δ | Provides End-to-End linker 4Δ for level 2 cloning |
| 153357 | pMAP L2B | Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication |
| 153358 | pMAP L2Γ | Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication |
| 153359 | pMAP L2Δ | Mobius Assembly cloning and expression vector for plants with high-copy number origin of replication |
| 153360 | pLXMA L2A | Expression vector for plants with medium-copy number origin of replication |
| 153361 | pLXMA_RK2 L2A | Expression vector for plants with low-copy number origin of replication |
| 153362 | mUAV | Converts DNA sequences into Phytobricks |
| 153363 | lvl0 UBQ10p | Plant promoter |
| 153364 | lvl0 MASp | Agrobacterium promoter for plant expression |
| 153365 | lvl0 UBQ11p | Plant promoter |
| 153366 | lvl0 UBQ4p | Plant promoter |
| 153367 | lvl0 OCSp | Agrobacterium promoter for plant expression |
| 153368 | lvl0 TCTPp | Plant promoter |
| 153369 | lvl0 35Sp | Cauliflower mosaic virus promoter for plant expression |
| 153370 | lvl0 G10-90p | Synthetic promoter for plant expression |
| 153371 | lvl0 ACT7p | Plant promoter |
| 153372 | lvl0 APT1p | Plant promoter |
| 153373 | lvl0 TUB9p | Plant promoter |
| 153374 | lvl0 ACT2p | Plant promoter |
| 153375 | lvl0 NOSp | Plant promoter |
| 153376 | lvl0 LEC2t | Plant terminator |
| 153377 | lvl0 FAD2t | Plant terminator |
| 153378 | lvl0 HSPt | Plant terminator |
| 153379 | lvl0 NDUFA8t | Plant terminator |
| 153380 | lvl0 G7t | Agrobacterium terminator for plant expression |
| 153381 | lvl0 MASt | Agrobacterium terminator for plant expression |
| 153382 | lvl0 35St | Cauliflower mosaic virus terminator for plant expression |
| 153383 | lvl0 UBQ5t | Plant terminator |
| 153384 | lvl0 ACT2t | Plant terminator |
| 153385 | lvl0 TUB9t | Plant terminator |
| 153386 | lvl0 APT1t | Plant terminator |
| 153387 | lvl0 RbcS2bt | Plant terminator |
| 153388 | lvl0 NOSt | Agrobacterium terminator for plant expression |
| 153389 | lvl0 NptII | Confers resistance to kanamycin and G418 |
| 153390 | lvl0 BlpR | Confers resistance to bialaphos or phosphinothricin (Glufosinate) |
| 153391 | lvl0 HptII | Confers resistance to hygromycin |
| 153392 | lvl0 turboRFP CDS | Red fluorescent protein coding sequence |
| 153393 | lvl0 turboRFP CTAG | Red fluorescent protein CTAG |
| 153394 | lvl0 mKate2 CDS | Far red fluorescent protein with SV40 NLS, codon optimised for Arabidopsis thaliana |
| 153395 | lvl0 eGFP CDS | Green fluorescent protein coding sequence |
| 153396 | lvl0 eYFP CTAG | Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana |
| 153397 | lvl0 eCFP CDS | Cyan fluorescent protein coding sequence |
| 153398 | lvl0 sfGFP CDS | Green fluorescent protein coding sequence |
| 153399 | lvl0 sfGFP CTAG | Green fluorescent protein CTAG |
| 153400 | lvl0 mTFP1 CTAG | Cyan fluorescent protein with FLAG tag |
| 153401 | lvl0 TagRFP CTAG | Red fluorescent protein CTAG codon optimised for Arabidopsis thaliana |
| 153402 | lvl0 YPet CTAG | Yellow fluorescent protein CTAG codon optimised for Arabidopsis thaliana |
| 153403 | lvl0 mVenus CDS | Yellow fluorescent protein coding sequence |
| 153404 | lvl0 nluc CDS | Nanoluciferase coding sequence |
| 153405 | lvl0 fluc CDS | Firefly luciferase coding sequence |
| 153406 | lvl0 sXVE | Estradiol inducible system trans activator |
| 153407 | lvl0 lexA-35S | Estradiol system inducible promoter |
| 153408 | lvl0 LhGR | Dex inducible system trans activator |
| 153409 | lvl0 pOp6-35S | Dex system inducible promoter |
| 153410 | lvl0 AlcR | Ethanol inducible system trans activator |
| 153411 | lvl0 alcSynth-34S | Ethanol system inducible promoter |
| 153412 | lvl0 MethylAblet | It is used to bypass the Level 1 cloning and directly feed terminators into Level 2 |