We narrowed to 20,110 results for: Cre

1. pRK-flag-USP19 494-1318

   Plasmid

   #78587

   Purpose

   Expression flag-USP19 494-1318 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 494-1318 (USP19 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   deleted amino acid 1-493

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

2. KHBD00433

   Plasmid

   #34538

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG9745 (D1 Fly)
   

   Use

   Gateway donor vector

   Available Since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

3. KP201: pMVP (L5-L4) eGFP-P2A

   Plasmid

   #121708

   Purpose

   pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   eGFP-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p3E-rev_U6_MCS

   Plasmid

   #109769

   Purpose

   Multisite gateway vector for 3' expression of a guide RNA from the U6 promoter (cassette in the reverse orientation). Contains BseRI sites for insertion of gRNA sequence

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   U6-gRNA cassette
   

   Use

   Zebrafish plasmids

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. KP301: pMVP (L5-L4) mCherry-P2A

   Plasmid

   #121710

   Purpose

   pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   mCherry-P2A (open)
   

   Use

   Synthetic Biology; Pmvp gateway entry plasmid

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGWB526

   Plasmid

   #74868

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with T7 (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOCC70

   Plasmid

   #118878

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
   

   Tags

   mGFP, cleavable with TEV protease; HIS6, cleavabl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLBT0160

   Plasmid

   #172812

   Purpose

   Level 1 module containing P19_P1 F

   Depositor

   Benjamin Field

   Article

   Velay et al Plant Methods. 2022 May 26;18(1):69. doi

   Insert

   p35S-5UTMV-CDS1_P19-3U+TERNOS
   

   Use

   Synthetic Biology

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUHG10.3-TetO-Rs1

   Plasmid

   #16313

   Depositor

   Bruce Conklin

   Article

   Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

   Insert

   h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (RS1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Added FLAG tag; Changed Aspartic Acid 100 to Alan…

   Available Since

   Jan. 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. HE401: pMVP (L1-R5) shRNA

    Plasmid

    #121698

    Purpose

    pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXylA-RgTALsyn

    Plasmid

    #100948

    Purpose

    Expression of Tyrosine Ammonia Lyase from constitutive XylA promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    XylA (Constitutive)

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CyPet-FKBP-E2

    Plasmid

    #175244

    Purpose

    Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycin

    Depositor

    Michael Rosen

    Article

    Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

    Insert

    UBE2I (UBE2I Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. actc1b-mKate2-rab8b

    Plasmid

    #109658

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab8b

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab8b (rab8b Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. actc1b-mKate2-rab1ab

    Plasmid

    #109606

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to rab1ab

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    rab1ab (rab1ab Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. secCitrine

    Plasmid

    #181964

    Purpose

    Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    signal peptide sequence from basic chitinase
    

    Tags

    Citrine YFP

    Expression

    Plant

    Promoter

    1.5 kb MUM4 promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. actc1b-BTK-mKate2

    Plasmid

    #109501

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to the PH domain from Human Bruton tyrosine kinase (BTK). Binds PtdIns(3,4,5)P3.

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    actc1b-BTK-mKate2
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-BE3-2NLS

    Plasmid

    #119031

    Purpose

    Stably express BE3 in mammalian cells

    Depositor

    Jinsong Li

    Article

    Li et al Nat Cell Biol. 2018 Oct 1. pii: 10.1038/

    Insert

    BE3
    

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. actc1b-mKate2-RhoAa

    Plasmid

    #109525

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to RhoAa

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    RhoAa (rhoaa Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E-FAPP1

    Plasmid

    #109555

    Purpose

    Multisite gateway vector for 3' tagging with the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3). Binds PtdIns4P

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    the PH domain from Human pleckstrin homology domain containing A3 (PLEKHA3).
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LF601: pMVP (L1-R5) TBG promoter

    Plasmid

    #121695

    Purpose

    pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TBG promoter (Liver specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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