166,818 results

1. pET28-his-3C_Tau352

   Plasmid

   #100092

   Purpose

   Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

   Depositor

   Nikolai Sluchanko

   Article

   Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

   Insert

   Fetal microtubule-associated protein tau (MAPT Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   wild type sequence

   Promoter

   T7

   Available Since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCer-Mcm5

   Plasmid

   #211391

   Purpose

   mammalian expression of human Mcm5 N-terminally fused to mCerulean

   Depositor

   Helmut Pospiech

   Insert

   Mcm5 (MCM5 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (AAV5)

   Viral Prep

   #121539-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) (#121539). In addition to the viral particles, you will also receive purified pOTTC1596 - pAAV SYN1 HA-hM3D(Gq) plasmid DNA. Synapsin-driven, HA-tagged hM3D(Gq) for neuronal activation. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Christopher Richie

   Article

   Galvan et al Eur J Neurosci. 2019 May 7. doi: 10.1111

   Promoter

   Syn

   Tags

   HA

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

4. iOn-CAG∞MCS

   Plasmid

   #154013

   Purpose

   Expression vector based on the iOn integration-coupled transcriptional switch (Kumamoto et al bioRxiv 2019), equipped with an MCS to clone-in genes of interest and express it from a CAG promoter

   Depositor

   Jean Livet

   Article

   Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d

   Insert

   MCS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBlueSKMimRNA

   Plasmid

   #102535

   Purpose

   Template for synthesis of Minos transposase mRNA

   Depositor

   Michalis Averof

   Article

   Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

   Insert

   Minos transposase mRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPGK-T7/2-CD44v8-10 (fl)

   Plasmid

   #137823

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v8-10 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length

   Promoter

   PGK

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3 Flag TSC2

   Plasmid

   #14129

   Depositor

   Brendan Manning

   Article

   Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

   Insert

   TSC2 (TSC2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBP-J23100

   Plasmid

   #72963

   Purpose

   Level 0 - J23100 standard iGEM promoter (5' CTAT/3' GTAC fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   J23100 promoter
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLC-Flag-CRBN-P2A-Hygro

   Plasmid

   #124303

   Purpose

   Lentiviral vector for expression of Flag tagged CRBN-P2A-Hygro casette from a CMV promoter

   Depositor

   Eva Gottwein

   Article

   Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

   Insert

   CRBN (CRBN Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF1335

    Plasmid

    #142786

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    POU3F2 (POU3F2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdestMB14_sur5p-dCas9-VP64

    Plasmid

    #177788

    Purpose

    Overexpression of dCas9-VP64 in C. elegans

    Depositor

    Michael Ristow

    Article

    Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

    Insert

    dCas9-VP64
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-RelA

    Plasmid

    #23255

    Depositor

    Warner Greene

    Article

    Chen Lf et al Science. 2001 Aug 31. 293(5535):1653-7.

    Insert

    p65 (RELA Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti Lifeact-EGFP BlastR

    Plasmid

    #84383

    Purpose

    Lentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neurons

    Depositor

    Ghassan Mouneimne

    Article

    Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

    Insert

    Lifeact
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    CMV immediate early

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1a_TauFRET_3xKQ_wpre

    Plasmid

    #207522

    Purpose

    Lentiviral construct for the expression of a FRET-based reporter for tau seeding in mammalian cells

    Depositor

    Bradley Hyman

    Article

    Lathuiliere et al Mol Neurodegener. 2023 Aug 8;18(1):53. d

    Insert

    TauFRET_P301L_3xKQ
    

    Use

    Lentiviral

    Tags

    NeonGreen and mTurquoise2

    Expression

    Mutation

    P301L; K311Q; K317Q; K321Q

    Promoter

    EF1a

    Available Since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-EGFP

    Plasmid

    #89684

    Purpose

    pCAG promoter driving EGFP

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Stx5S-mCherry

    Plasmid

    #165087

    Purpose

    Expresses Stx5S-mCherry in mammalian cells

    Depositor

    Geert van den Bogaart

    Article

    Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

    Insert

    Stx5 (Short isoform) (STX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Lacks amino acids 1-54, enocdes short isoform of …

    Promoter

    CMV

    Available Since

    Nov. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHBS1389 IBB-GFP-mCherry3E

    Plasmid

    #118803

    Purpose

    Fluorescence-based TDP43-dependent splicing reporter

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    Synthetic
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. LAMP-mCherry-CRY2

    Plasmid

    #102249

    Purpose

    Expresses fusion of CRY2PHR with mCherry and LAMP1 (1-415)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    LAMP-mCherry-CRY2 (LAMP1, CRY2 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R

    Plasmid

    #228726

    Purpose

    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A D56R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R

    Plasmid

    #228725

    Purpose

    Expresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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