164,516 results
-
Plasmid#144389PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertZNF658 (ZNF658 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Tet
Plasmid#138086PurposeEmpty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particlesDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterminimal CMV promoter with heptamerized tet‐operat…Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(4)P Probe
Plasmid#211509PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteriaDepositorInsertSidC (P4C)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiVIPe4_GCaMP6f
Plasmid#213859PurposeAAV construct expressing GCaMP6f driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_GCaMP6fDepositorInsertGCaMP6f
UseAAVTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPV-Dual_promoter-EF1α-2xNLS-Cascade+Cas3-P (RD)
Plasmid#204619PurposeExpression vector of Cascade and Cas3. Puromycin selectable.DepositorInsertCas7, Cas5, Cas8, Cas11, Cas6, and Cas3
UseCRISPRTagsEach protein has C- and N-terminal SV40 NLSExpressionMutationPromoterEF1aAvailable sinceAug. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Artichoke
Plasmid#73320PurposeArtichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)DepositorTypeEmpty backboneUseLentiviralTagsEGFPExpressionMammalianMutationPromoterSFFV and EF1aAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_gRNA-Puro
Plasmid#84752PurposeExpresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoterDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
GB1-A11(2-196, D40G)-Strep
Plasmid#196210PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
UseTagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationD40GPromoterAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCSC-SP-PW-Nep (aka: pBOB-NEP)
Plasmid#12338DepositorInsertNEP (MME Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCI-VSVG
Plasmid#1733PurposeVSV-G expressing envelope vector; recommended for the FELIX (FIV-based) vector system from the Nolan labDepositorInsertVSV-G Envelope
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-U6gRNA(lib)-PGKpuroT2ABFP library
Pooled Library#104861DepositorAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-BAZ1A
Plasmid#65371PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorInsertBAZ1A (BAZ1A Human)
UseTagsEmGFP and V5ExpressionMammalianMutation796I compared to all reference sequencesPromoterCMVAvailable sinceJune 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-EGFP-RAB5A-IRES-Puromycin
Plasmid#134858PurposeExpresses EGFP-tagged early endosome markerDepositorInsertRas-related protein 5A
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1aAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBWB162
Plasmid#140634PurposepBAV1k-lacI-Trc-mCherry base, lacI repression vector for ADP1DepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-mIL2
Plasmid#74467Purposeexpression of mouse IL2 in mammalian cellsDepositorInsertIL2 (Il2 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)
Plasmid#182652PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
4xU6-tRNAM15
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCCL-c-MNDU3-X
Plasmid#81071PurposeEmpty lentiviral backbone with the MNDU3 promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterMNDU3Available sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF0985
Plasmid#142249PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TGFB1
Plasmid#116799PurposeLentiviral expression of TGFB1DepositorInsertTGFB1 (TGFB1 Human)
UseLentiviralTagsExpressionMutationWTPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only