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181,096 results

Showing: 8061 - 8080 of 181096 results

  3. p2CT-His-MBP-Lbu_C2c2_R472A_H477A_R1048A_H1053A

    Plasmid
    #83485
    Purpose
    Bacterial expression of L. buccalis C2c2 CRISPR effector (double HEPN nuclease 1 and 2 inactive mutant).
    Depositor
    Jennifer Doudna
    Publication
    East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature
    Insert
    Lbu_C2c2_R472A_H477A_R1048A_H1053A
    Use
    CRISPR
    Tags
    His6-MBP
    Expression
    Bacterial
    Mutation
    R472A, H477A, R1048A, H1053A
    Available since
    Oct. 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83485-plasmid-map-sequence-id-159341

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  8. EasyClone 2.0 Yeast Toolkit

    Plasmid kit
    #1000000073
    Purpose
    An expanded toolkit of integrative vectors for stable gene expression in S. cerevisiae.
    Depositor
    Irina Borodina
    Application
    Cloning and Synthetic Biology
    Vector type
    Yeast Expression
    Cloning type
    USER
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pUV15-pHGFP

    Plasmid
    #70045
    Purpose
    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells
    Depositor
    Sabine Ehrt
    Publication
    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    phGFP
    Use
    Mycobacterium tuberculosis expression vector
    Promoter
    mycobacterial promoter Psmyc
    Available since
    Oct. 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70045-plasmid-map-sequence-id-488289

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  16. Vimentin - APEX2 in pECFP

    Plasmid
    #66170
    Purpose
    APEX2 fused to the C-terminus human vimentin
    Depositor
    Alice Ting
    Publication
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Vimentin - APEX2 (VIM Synthetic, Human)
    Tags
    Flag epitope tag (DYKDDDDK)
    Expression
    Mammalian
    Mutation
    APEX2 contains 4 mutations relative to wt soybean…
    Promoter
    CMV
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    66170-plasmid-map-sequence-id-113682

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  17. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    62200-plasmid-map-sequence-id-395472

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Showing: 8061 - 8080 of 181096 results