166,818 results

1. pET41b+_Npl4

   Plasmid

   #117108

   Purpose

   Production of recombinant Npl4 in E.coli

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   Npl4 (NPLOC4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-Ef1a-Coff/Fon-GCaMP6F

   Plasmid

   #137124

   Purpose

   Intersectional viral expression of GCaMP6F in cells expressing Flp AND NOT Cre

   Depositor

   INTRSECT 2.0 Project, Karl Deisseroth

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-GCaMP6F
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Tags

   Expression

   Mutation

   Removed RSET tag

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-DFCP1

   Plasmid

   #86746

   Purpose

   N-terminal mCherry-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human DFCP1 (ZFYVE1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-Tnos

   Plasmid

   #211843

   Purpose

   Plant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   LRR-NEL
   

   Use

   Synthetic Biology

   Tags

   HA and mCherry

   Expression

   Plant

   Mutation

   C492A

   Promoter

   UBIQUITIN10

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWZL Neo Myr Flag BTK

   Plasmid

   #20432

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   BTK (BTK Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pAW014-2

   Plasmid

   #85612

   Purpose

   Multi-gRNA delivery vector containing ugtP-gRNA.P395T for Bacillus subtilis

   Depositor

   Perry Chou

   Article

   Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

   Insert

   ugtP-gRNA.P395T
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-APEX2-NIK3x

   Plasmid

   #129274

   Purpose

   Expression of APEX2 in the nucleolus

   Depositor

   Alice Ting

   Article

   Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

   Insert

   GFP-APEX2-NIK3x
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV_hSynapsin_psychLight2 (AAV9)

   Viral Prep

   #163909-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV_hSynapsin_psychLight2 (#163909). In addition to the viral particles, you will also receive purified pAAV_hSynapsin_psychLight2 plasmid DNA. Synapsin-driven expression of the genetically encoded fluorescent sensor psychLight2 to detect behaviorally relevant serotonin release. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Dong et al Cell. 2021 Apr 8. pii: S0092-8674(21)003

   Promoter

   Syn

   Tags

   None

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

9. pK27Sumo_His-SUMO-PLpro (nsp3) (SARS-CoV-2)

   Plasmid

   #169193

   Purpose

   To express SARS-CoV-2 Papain-like protease in E. coli

   Depositor

   John Diffley

   Article

   Lim et al Biochem J. 2021 Jul 16;478(13):2517-2531

   Insert

   14His-SUMO-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
   

   Use

   Tags

   14His-SUMO

   Expression

   Bacterial

   Mutation

   Codon optimised for E. coli

   Promoter

   T5

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG_attP

    Plasmid

    #110095

    Purpose

    Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAcGFP1-N1-Dsup-C

    Plasmid

    #90022

    Purpose

    To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

    Insert

    Dsup
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    C-terminal region alone

    Promoter

    CMV

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-ERK2

    Plasmid

    #37145

    Depositor

    Rony Seger

    Article

    Rubinfeld et al J Biol Chem. 1999 Oct 22;274(43):30349-5

    Insert

    ERK2 (MAPK1 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    aa1-6 deleted as a result of cloning strategy

    Promoter

    CMV

    Available Since

    Nov. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pACλCI-β-flap (831-1057)

    Plasmid

    #53733

    Purpose

    Expression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    λCI-β-flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac UV5

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBRα-β flap (831-1057)

    Plasmid

    #53734

    Purpose

    Expression of alpha NTD fused via three alanines to E. coli RNAP subunit beta, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.

    Depositor

    Ann Hochschild

    Article

    Dove et al Nature. 1997 Apr 10;386(6625):627-30.

    Insert

    alpha NTD-Beta flap fusion
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lpp/lacUV5 (tandem promoter)

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. EBFP2-H2B-6

    Plasmid

    #55243

    Purpose

    Localization: Nucleus/Histones, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    D26G and V119I in H2B

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pAG153 FKBP-CFAST11

    Plasmid

    #130813

    Purpose

    Expresses FKBP-CFAST11 in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FKBP-CFAST11
    

    Use

    Tags

    Myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. 7TGC

    Plasmid

    #24304

    Depositor

    Roel Nusse

    Article

    Fuerer et al PLoS One. 2010 . 5(2):e9370.

    Insert

    7xTcf-eGFP // SV40-mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    EGFP is GFP with S65T and F64L mutations

    Promoter

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKM468-EGFP

    Plasmid

    #108434

    Purpose

    ORBIT integrating plasmid for C-terminal tagging with EGFP.

    Depositor

    Kenan Murphy

    Article

    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

    Insert

    attB-eGFP-4xGly-TEV-Flag-6xHis
    

    Use

    Orbit integrating plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEIAV-SIN6.1 CGFPW

    Plasmid

    #44171

    Depositor

    John Olsen

    Article

    McKay et al Gene Ther. 2006 Apr;13(8):715-24.

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    chromophore mutations

    Promoter

    CMV

    Available Since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA (AAV5)

    Viral Prep

    #230681-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA (#230681). In addition to the viral particles, you will also receive purified AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA plasmid DNA. minBG-driven expression of iCre recombinase in specific populations of brain cells. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Bosiljka Tasic, Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Promoter

    minBG

    Tags

    None

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only