168,823 results

1. pVRC8400-SARS-CoV-2-RBD-AVI

   Plasmid

   #160476

   Purpose

   Expresses SARS-CoV-2 RBD domain with single chain Fc, HRV3C protease cleavage site, and AVI tag

   Depositor

   Peter Kwong

   Article

   Zhou et al Cell Rep. 2020 Oct 27;33(4):108322. doi:

   Insert

   SARS-CoV-2-RBD
   

   Tags

   AVI, HRV3C, and scFc

   Expression

   Mammalian

   Available Since

   Oct. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Multiplex CRISPR/Cas9 Assembly System Kit

   Plasmid Kit

   #1000000055

   Purpose

   Golden Gate cloning assembly for construction of all-in-one CRISPR/Cas9 vectors expressing multiple gRNAs with either Cas9 nuclease or Cas9 D10A nickase; see kit #1000000062 for FokI Accessory Pack

   Depositor

   Takashi Yamamoto

   Available Since

   Nov. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. Ezh2[FL]-dCas9

   Plasmid

   #100086

   Purpose

   Full-length [FL] mouse Ezh2 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

   Depositor

   David Segal

   Article

   O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

   Insert

   14056 (Ezh2 Mouse)
   

   Use

   CRISPR

   Tags

   3XFLag-NLS-Ezh2[FL]-dCas9-NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET24c(+)-EFTu

   Plasmid

   #31172

   Depositor

   Linda Spremulli

   Article

   Zhang et al FEBS Lett. 1996 Aug 12. 391(3):330-2.

   Insert

   E. coli elongation factor Tu (tufB E. coli)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

5. SpyTag-sfGFP

   Plasmid

   #184226

   Purpose

   Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.

   Depositor

   Mark Howarth

   Article

   Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:

   Insert

   SpyTag-sfGFP
   

   Tags

   His6, SpyTag, and TEV protease cleavage site

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Fuw-dCas9-Dnmt3a

   Plasmid

   #84476

   Purpose

   Lentiviral construct to express dCas9 fused with Dnmt3a

   Depositor

   Rudolf Jaenisch

   Article

   Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

   Insert

   dCas9-Dnmt3a (DNMT3A Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKB01_mCherry

   Plasmid

   #69104

   Purpose

   Expresses mCherry (codon adapted for Bacillus subtilis); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.

   Depositor

   Jan-Willem Veening

   Article

   Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:

   Insert

   mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIS3p:CloverGFP-Tub1+3'UTR::URA3

   Plasmid

   #50636

   Depositor

   Wei-Lih Lee

   Article

   Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

   Insert

   CloverGFP-Tub1
   

   Use

   Yeast expression

   Tags

   CloverGFP

   Promoter

   HIS3p

   Available Since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLIK-GFP

   Plasmid

   #66844

   Purpose

   Tet-inducible expression of GFP in mammalian cells

   Depositor

   Christian Metallo

   Article

   Lewis et al Mol Cell. 2014 Jul 17;55(2):253-63. doi:

   Insert

   GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pwB3b

    Plasmid

    #32106

    Depositor

    Christopher Buck

    Article

    Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

    Inserts

    BKV VP1
    BKV VP3
    

    Expression

    Mammalian

    Mutation

    codon modified

    Available Since

    Oct. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFGC-pcoCas9

    Plasmid

    #52256

    Purpose

    A binary plasmid containing 35SPPDK:pcoCas9:NOS cassette for plant expression of Cas9 and multiple cloning sites for insertion of guide RNA

    Depositor

    Jen Sheen

    Insert

    35SPPDK:pcoCas9:NOS cassette
    

    Use

    CRISPR; Plant expression

    Promoter

    constitutive 35SPPDK promoter

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pComb3HSS

    Plasmid

    #64133

    Purpose

    2nd generation plasmid for phage display on modified geneIII, contains stuffer fragment

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Inserts

    SS Stuffer
    Light Chain Stuffer
    Heavy Chain Stuffer
    

    Use

    Phage display

    Tags

    geneIII

    Expression

    Bacterial

    Promoter

    lacZ

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSC101-AmpR EV

    Plasmid

    #208184

    Purpose

    empty plasmid vector, non-fluorescent

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Type

    Empty backbone

    Use

    Reporter

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+StayGold-C Cloning Vector

    Plasmid

    #208337

    Purpose

    To express a protein of interest fused to the C-terminus of StayGold in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Tags

    StayGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. enAsABE8e

    Plasmid

    #138506

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-dAsCas12a (En)
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC18R6K-mini-Tn7T-Gm

    Plasmid

    #65022

    Purpose

    GmR mini-Tn7 vector, R6K replicon

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Available Since

    Aug. 17, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pSBtet-RN

    Plasmid

    #60503

    Purpose

    SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Expression

    Mammalian

    Promoter

    TCE & RPBSA

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBT120a17

    Plasmid

    #138510

    Purpose

    P1

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    gIII-xLuxAB
    

    Use

    Synthetic Biology

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_miniENVLPE

    Plasmid

    #232431

    Purpose

    Expression plasmid to produce miniENVLPE particles

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    miniENVLPE
    

    Expression

    Mammalian

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB_mU6_enh-gRNA_Puro-T2A-BFP

    Plasmid

    #235571

    Purpose

    Enhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.

    Depositor

    Jamie Hackett

    Article

    Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

    Insert

    sgRNA backbone
    

    Use

    CRISPR

    Available Since

    April 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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