166,818 results

1. pSL3718 (pQCascade_Pse)

   Plasmid

   #200899

   Purpose

   Expresses human codon-optimized Pseudoalteromonas Cas7, Cas8, Cas6, and TniQ from a CMV promoter, in a polycistronic vector format separated by T2A sequences.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCas7, PseCas8, PseCas6, PseTniQ
   

   Use

   Tags

   NLS (TniQ) and NLS, T2A (Cas7, Cas8, Cas6)

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-spCas9-ABE-Umax-rest1

   Plasmid

   #222144

   Purpose

   Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9
   

   Use

   CRISPR; Zebrafish expression

   Tags

   Bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   July 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1)

   Viral Prep

   #172921-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (#172921). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor jGCaMP8s in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Promoter

   Syn

   Tags

   mRuby3

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

4. pENN.AAV.hSyn.Cre.hGH (AAV9)

   Viral Prep

   #105555-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pENN.AAV.hSyn.Cre.hGH (#105555). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.Cre.hGH plasmid DNA. hSyn driven Cre expression. These AAV preparations are suitable purity for injection into animals.

   Depositor

   James M. Wilson

   Promoter

   Syn

   Tags

   None

   Available Since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

5. pLenti_EF1a_Flag_TP53_WT

   Plasmid

   #183112

   Purpose

   Expresses flag-tagged p53 in mammalian cells

   Depositor

   William Kaelin

   Article

   Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

   Insert

   TP53 (TP53 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   See Depositor Comments Below

   Promoter

   EF1a

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5F-TACE

   Plasmid

   #31713

   Purpose

   Mammalian expression of human TACE (ADAM17)

   Depositor

   Rik Derynck

   Article

   Liu et al Mol Cell. 2009 Jul 10. 35(1):26-36.

   Insert

   TACE (ADAM17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 13, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pENTR TERRA

   Plasmid

   #111383

   Purpose

   TERRA with approximately 130 telomere hexanucleotide repeats of TTAGGG and TTGGGG variant sequences

   Depositor

   Klaus Holzmann

   Article

   Kreilmeier et al Genes (Basel). 2016 Aug 16;7(8). pii: ge

   Insert

   TERRA
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMC-1-ZmPLTP-6

   Plasmid

   #197713

   Purpose

   Phytobrick (MoClo) Level 0 Part

   Depositor

   Daniel Voytas

   Article

   Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

   Insert

   ZmPLTP promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPGK-T7/2-CD44v9 (fl)

   Plasmid

   #137818

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v9 (fl) (CD44 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and mutation H48L

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-GFP-IRES-CRE

    Plasmid

    #48201

    Purpose

    retroviral vector encoding for GFP and Cre-recombinase

    Depositor

    Fred Gage

    Article

    Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

    Inserts

    eGFP
    Cre
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and linked by IRES

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJG100

    Plasmid

    #138966

    Purpose

    Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available Since

    July 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. YE1-BE4max

    Plasmid

    #138155

    Purpose

    C-to-T base editor

    Depositor

    David Liu

    Article

    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

    Insert

    rAPOBEC1(YE1)-nSpCas9-UGI-UGI
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. NAX_CAGGS_CAS9_mcherry

    Plasmid

    #167862

    Purpose

    PiggyBac compatible plasmid expressing spCas9

    Depositor

    Nan Cher Yeo, Alejandro Chavez

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. betaTrCP mVenus reporter

    Plasmid

    #192458

    Purpose

    Fluorescent reporter for beta-TrCP activity in human cells

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CDC25B (CDC25B Human)
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338

    Promoter

    EF1alpha

    Available Since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. betaTrCP mVenus mutant reporter

    Plasmid

    #192459

    Purpose

    Fluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.

    Depositor

    Steven Cappell

    Article

    Paul et al Nat Commun. 2022 Oct 26;13(1):6364. doi:

    Insert

    CdC25B
    

    Use

    Lentiviral

    Tags

    SV40 NLS and mVenus

    Expression

    Mammalian

    Mutation

    aa198-338 of CDC25B. betaTrCP degron sequence (aa…

    Promoter

    EF1alpha

    Available Since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. 6PBG

    Plasmid

    #162263

    Purpose

    Bacterial Expression

    Depositor

    Cheryl Arrowsmith

    Insert

    COPA (COPA Human)
    

    Use

    Baculovirus expresssion

    Tags

    6xHis-TEV

    Expression

    Mutation

    Promoter

    Polyhedrin

    Available Since

    Dec. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. mCherry-CD9-10

    Plasmid

    #55013

    Purpose

    Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    CD9 (CD9 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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