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Plasmid#64247Purposeexpresses sgRNA under U6b promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceMay 4, 2015AvailabilityAcademic Institutions and Nonprofits only
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pJEP314-pAAV-U6SaCas9gRNA(SapI)-CMV-SaCas9-DIO-pA
Plasmid#113691PurposeU6 driven SaCas9 gRNA expression cassette followed by a CMV driven inverted SaCas9. SaCas9 is floxed to render the system cre-dependent.DepositorInsertSaCas9
UseAAV, CRISPR, and Cre/LoxTagsNLSExpressionMutationPromoterCytomegalo Virus(CMV)Available sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔG
Plasmid#168998PurposeExpresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseUnspecifiedTagsEGFP and mRFP1ExpressionMutationPromoterAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-teLuc c-myc
Plasmid#100026PurposeEnhanced red-shifted bioluminescence when paired with diphenylterazine (DTZ)DepositorInsertteLuc, a NanoLuc mutant
UseTagsc-mycExpressionMammalianMutationD19S, D85N, C164HPromoterCMVAvailable sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_AOX-FLAG
Plasmid#177984PurposeAll-in-one dox-inducible expression of Alternative OxidaseDepositorInsertAOX
UseLentiviralTagsFLAGExpressionMammalianMutationCodon optimized cDNA for expression in human cellsPromoterTREAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
Plxna1-Fc-His
Plasmid#72122PurposeExpresses the extracellular region of the PlexinA1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxna1 (Plxna1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFL
Plasmid#110739PurposePlasmid for the insect cell expression system, acceptor for plasmid fusions, two promoters, Multiplication Module M, Tn7 transposition and LoxP siteDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterPolyhedrin and p10 promotersAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGIP
Plasmid#26777PurposeLentiviral vector for stable integration of GFP expression cassette with puromycin selection.DepositorInsertEF1a-GFP-IRES-Puro (gfp )
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HsPeg10rc3
Plasmid#174859PurposeExpresses recoded human Peg10 in mammalian cellsDepositorInsertrecoded Homo sapiens PEG10 (PEG10 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZ P (cHS4)X4 OCT4p eGFP SV40pA v2
Plasmid#115518PurposeDonor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows OCT4 dependent expression of GFP, and contains cHS4 insulators.DepositorInserteGFP
UseAAV; Donor plasmid for recombinase-mediated casse…TagsExpressionMammalianMutationPromoterAvailable sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-L2-TALEN
Plasmid#51554PurposeExpresses the H11-L2 TALEN in mammalian cells. This TALEN was coexpressed with H11-R2 (plasmid #51555) to cleave the H11 locus.DepositorInsertH11-L2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTCF236 HLA-B*40:01
Plasmid#180463PurposeE. coli expression of soluble MHC proteinDepositorInsertHLA-B*40:01
UseTagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…PromoterAvailable sinceFeb. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorInsertH11-R2 TALEN
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUCP20T-E2Crimson
Plasmid#78473PurposeBroad range bacterial fluorescence labelingDepositorInsertE2-Crimson
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-SopF
Plasmid#135174PurposeExpresses an mCherry-SopF fusion in mammalian cellsDepositorInsertSopF (STM1239 Salmonella enterica serovar Typhimurium)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-EcSTH-FLAG
Plasmid#218628PurposeThis plasmid contains human codon optimized sequence of EcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.DepositorInsertEcSTH
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
TRIPZ-bioGEF-UBwt-PURO
Plasmid#208045PurposeEnables inducible expression of low-affinity Avitag-Ubwt; to perform bioE3; selection with puromycinDepositorInsertUb (UBC Human)
UseLentiviralTagsLow-affinity AvitagExpressionMutationPromotertetO/UBCAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC18-GFP
Plasmid#220495PurposeTo generate substrates to monitor Ku overloading and its impact on transcription at the DNA ends vicinity.DepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterSV40Available sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
HRE-UnaG
Plasmid#201710PurposeHypoxia responsive promoter driving UnaG fluorescent proteinsDepositorInsertUnaG
UseTagsExpressionMammalianMutationPromoterHRE-mCMVAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1 Hepatitis C Virus NS3/NS4A
Plasmid#61696Purposeinducible dual expression of NS3 protease along with its cofactor NS4ADepositorInsertsNS4A
NS3
UseTagshisExpressionBacterialMutationPromoterT7Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only