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GFP-UBR5 ∆HECT
(Plasmid #52051)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 52051 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA 6.2 N-EmGFP-DEST-V5
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5887
  • Total vector size (bp) 14287
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Large plasmid. Can be difficult to produce high yield.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UBR5 ∆HECT
  • Alt name
    EDD
  • Alt name
    EDD1
  • Alt name
    hHYD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8400
  • Mutation
    C2769A
  • GenBank ID
    NM_001282873.1
  • Entrez Gene
    UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Plasmid encodes a C-terminal V5 fusion, however this is not included as UBR5 cDNA insert contains 3' STOP codon.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-UBR5 ∆HECT was a gift from Darren Saunders (Addgene plasmid # 52051 ; http://n2t.net/addgene:52051 ; RRID:Addgene_52051)
  • For your References section:

    TRIP12 and UBR5 suppress spreading of chromatin ubiquitylation at damaged chromosomes. Gudjonsson T, Altmeyer M, Savic V, Toledo L, Dinant C, Grofte M, Bartkova J, Poulsen M, Oka Y, Bekker-Jensen S, Mailand N, Neumann B, Heriche JK, Shearer R, Saunders D, Bartek J, Lukas J, Lukas C. Cell. 2012 Aug 17;150(4):697-709. doi: 10.1016/j.cell.2012.06.039. Epub 2012 Aug 9. 10.1016/j.cell.2012.06.039 PubMed 22884692