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pCMV-Tag2B EDD
(Plasmid #37188)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37188 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCMV-Tag2B
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 4373
  • Total vector size (bp) 12770
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EDD
  • Alt name
    UBR5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    8397
  • Mutation
    Please see Depositor Comments
  • Entrez Gene
    UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • 6x His Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ApaI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer T7 Forward
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    Amanda Russell, Cancer Research Program, Garvan Institute of Medical Research, NSW, Australia
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

Addgene NGS results identified a K503R mutation within the UBR5 translation. There is no information on the functional consequence of this mutation

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-Tag2B EDD was a gift from Darren Saunders & Charles Watts (Addgene plasmid # 37188 ; http://n2t.net/addgene:37188 ; RRID:Addgene_37188)
  • For your References section:

    EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response. Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, Calanni ST, Jans DA, Sutherland RL, Watts CK. J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13. 10.1074/jbc.M203527200 PubMed 12011095