Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

p6351 MSCV-CMV-CMV-Flag-HA-NSD3
(Plasmid #31357)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31357 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST-iTAP
  • Backbone size w/o insert (bp) 8700
  • Modifications to backbone
    Note that this is a Gateway cloning vector. The insert replaces a large (approx. 1.7kb) cassette between the attR sites. Please take this into consideration when designing diagnostic digests.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NSD3
  • Species
    H. sapiens (human)
  • Entrez Gene
    NSD3 (a.k.a. KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328)
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p6351 MSCV-CMV-CMV-Flag-HA-NSD3 was a gift from Peter Howley (Addgene plasmid # 31357 ; http://n2t.net/addgene:31357 ; RRID:Addgene_31357)
  • For your References section:

    The Brd4 Extraterminal Domain Confers Transcription Activation Independent of pTEFb by Recruiting Multiple Proteins, Including NSD3. Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM. Mol Cell Biol. 2011 Jul . 31(13):2641-52. 10.1128/MCB.01341-10 PubMed 21555454