|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31357||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8700
Modifications to backboneNote that this is a Gateway cloning vector. The insert replaces a large (approx. 1.7kb) cassette between the attR sites. Please take this into consideration when designing diagnostic digests.
Vector typeMammalian Expression
Growth in Bacteria
SpeciesH. sapiens (human)
Entrez GeneNSD3 (a.k.a. KMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328)
/ Fusion Proteins
- FLAG (N terminal on backbone)
- HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMVf
- 3′ sequencing primer MSCV-2 (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p6351 MSCV-CMV-CMV-Flag-HA-NSD3 was a gift from Peter Howley (Addgene plasmid # 31357 ; http://n2t.net/addgene:31357 ; RRID:Addgene_31357)
For your References section:The Brd4 Extraterminal Domain Confers Transcription Activation Independent of pTEFb by Recruiting Multiple Proteins, Including NSD3. Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM. Mol Cell Biol. 2011 Jul . 31(13):2641-52. 10.1128/MCB.01341-10 PubMed 21555454