p6345 MSCV-CMV-Flag-HA-Brd4 1-722
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||31352||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5000
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Gene/Insert nameBrd4 1-722
SpeciesH. sapiens (human)
Insert Size (bp)4100
/ Fusion Proteins
- FLAG (N terminal on backbone)
- HA (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ sequencing primer CMVf
- 3′ sequencing primer MSCV-2 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
*The MSCV-CMV-Brd4-1-722 was made from pENTR-Brd4 1-722. But the gateway LR recombination reaction seems to select for the internal aa 719 position DNA sequence, and the insert for this plasmid actually only codes up to amino acid 719. The name has been kept for consistency.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p6345 MSCV-CMV-Flag-HA-Brd4 1-722 was a gift from Peter Howley (Addgene plasmid # 31352 ; http://n2t.net/addgene:31352 ; RRID:Addgene_31352)
For your References section:The Brd4 Extraterminal Domain Confers Transcription Activation Independent of pTEFb by Recruiting Multiple Proteins, Including NSD3. Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM. Mol Cell Biol. 2011 Jul . 31(13):2641-52. 10.1128/MCB.01341-10 PubMed 21555454