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p6345 MSCV-CMV-Flag-HA-Brd4 1-722
(Plasmid #31352)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 31352 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV-CMV
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Brd4 1-722
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4100
  • Mutation
    *see comments
  • Entrez Gene
    BRD4 (a.k.a. CAP, CDLS6, FSHRG4, HUNK1, HUNKI, MCAP)
  • Tags / Fusion Proteins
    • FLAG (N terminal on backbone)
    • HA (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*The MSCV-CMV-Brd4-1-722 was made from pENTR-Brd4 1-722. But the gateway LR recombination reaction seems to select for the internal aa 719 position DNA sequence, and the insert for this plasmid actually only codes up to amino acid 719. The name has been kept for consistency.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p6345 MSCV-CMV-Flag-HA-Brd4 1-722 was a gift from Peter Howley (Addgene plasmid # 31352 ; http://n2t.net/addgene:31352 ; RRID:Addgene_31352)
  • For your References section:

    The Brd4 Extraterminal Domain Confers Transcription Activation Independent of pTEFb by Recruiting Multiple Proteins, Including NSD3. Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM. Mol Cell Biol. 2011 Jul . 31(13):2641-52. 10.1128/MCB.01341-10 PubMed 21555454