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pMT3-RhoGC D380E

Plasmid

#100035

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC D380E

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

D380E

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pMT3-RhoGC D380N

Plasmid

#100034

Purpose

This plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.

Depositor

Daniel Oprian

Article

Trieu et al J Biol Chem. 2017 Jun 23;292(25):10379-1

Insert

RhoGC D380N

Tags

1D4 (TETSQVAPA)

Expression

Mammalian

Mutation

D380N

Promoter

Adenovirus major late promoter

Available Since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX4T-Mst2 SARAH

Plasmid

#84298

Purpose

Expresses Mst2 SARAH domain for interaction and spectroscopy studies.

Depositor

Jonathan Chernoff

Article

Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

Insert

Mst2 (STK3 Human)

Tags

GST

Expression

Bacterial

Mutation

L443W

Promoter

tac

Available Since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
GST-CHMP4A (205-222) (pGEX2T(TEV))

Plasmid

#80633

Purpose

Residues 205-222 of CHMP4A, Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-222;

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO/FLAG-hPMR1(2H)-Tev-Bio

Plasmid

#80325

Purpose

Expresses a catalytically inactive form of human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tags

Depositor

Daniel Schoenberg

Article

Gu et al RNA. 2012 Jun;18(6):1186-96. doi: 10.126

Insert

PXDNL-003 (PXDNL Human)

Tags

Biotin

Expression

Mammalian

Mutation

H170A and H256A

Promoter

CMV

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)

Plasmid

#70195

Purpose

Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer extension), 21 (linker from primer extension + deGFP), and 105 (vector

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR-deGFP fusion

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-mOC-STAMP (N162D)-GFP

Plasmid

#73580

Purpose

Lentivirus expression of mOC-STAMP with N162D mutation

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

Insert

OC-STAMP (Ocstamp Mouse)

Use

Lentiviral

Tags

GFP

Mutation

changed asparagin (N) 162 to Aspartic Acid (D)

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
MT79

Plasmid

#63112

Purpose

The artificial microRNA is designed for down-regulating expression of ACD6 mRNA.

Depositor

Detlef Weigel

Article

Todesco et al Nature. 2010 Jun 3;465(7298):632-6. doi:

Insert

amiR-ACD6

Expression

Plant

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGS1412

Plasmid

#63787

Purpose

Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCitrine Strep-tag II followed by URA3 selection marker

Depositor

Monika Golas, Bjoern Sander

Article

Rai et al Mol Biotechnol. 2014 Jun 27.

Insert

eCitrine-Strep-tag II followed by URA3 selection marker

Use

E. coli cloning vector

Tags

eCitrine-Strep-tag II

Promoter

none

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pET-22HT-MSI1(7-192)Q30D

Plasmid

#60346

Purpose

Q30D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

Q30D; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Dec. 1, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E

Plasmid

#60352

Purpose

R53E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G64A

Plasmid

#60351

Purpose

G64A mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G64A; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)H83L

Plasmid

#60350

Purpose

H83L mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

H83L; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G35D

Plasmid

#60347

Purpose

G35D mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G35D; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)G35E

Plasmid

#60348

Purpose

G35E mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

G35E; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)H83F

Plasmid

#60349

Purpose

H83F mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Tags

His and TEV protease site

Expression

Bacterial

Mutation

H83F; contains amino acids 7-192 of Msi1

Promoter

T7

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only
Cs2+Etv4 S100D

Plasmid

#49094

Purpose

expression of Etv4 S100D mutant

Depositor

Michael Tsang

Article

Znosko et al Dev Biol. 2010 Jun 1;342(1):11-25. Epub

Insert

Etv4 S100D (etv4 Zebrafish)

Use

Xenopus/avian/zebrafish

Expression

Mammalian

Mutation

S100D

Promoter

CMV

Available Since

Nov. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pTYB-pLAC-F112S

Plasmid

#48727

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 112 from Phenylalanine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Phenylalanine 12 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB-pLAC-L108S

Plasmid

#48725

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 108 from Leucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Leucine 108 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only
pTYB1-pLAC-I98S

Plasmid

#48723

Purpose

Bacterial expression of Lacritin Protein with Mutation at protein 98 from Isoleucine to Serine

Depositor

Gordon Laurie

Article

Wang et al J Biol Chem. 2013 Jun 21;288(25):18146-6

Insert

Lacritin (LACRT Human)

Tags

Intein

Expression

Bacterial

Mutation

Isoleucine 98 to Serine

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only