We narrowed to 1,068 results for: gibson assembly

1. 6xHis-TEV-GEI-17(133-509)

   Plasmid

   #87056

   Purpose

   Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

   Depositor

   Ronald Hay

   Article

   Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

   Insert

   GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
   

   Use

   Tags

   6xHis-TEV and GAGGGS linker

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHS1742

   Plasmid

   #239838

   Purpose

   OrufIscB-KRK mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   OrufIscB-KRK
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1 + E137K/E4…

   Promoter

   CMV

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS2096

   Plasmid

   #239835

   Purpose

   NovaIscB E. coli expression for recombinant purification

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB
   

   Use

   Tags

   14xHis-TwinStrep-bdSUMO

   Expression

   Bacterial

   Mutation

   Insertion of REC domain from NbaCas9-1 and swap o…

   Promoter

   T7

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pnCS_mEGFP-DSep2_Peanut-Strep

   Plasmid

   #174496

   Purpose

   bacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila Peanut

   Depositor

   Manos Mavrakis

   Article

   Mavrakis et al Methods Cell Biol. 2016;136:199-220. doi

   Inserts

   Sep2 (Sep2 Fly)
   peanut (pnut Fly)
   

   Use

   Tags

   Strep and mEGFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. deltaVP1 + VP2C-MIOX

   Plasmid

   #166680

   Purpose

   Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

   Inserts

   VP2C-MIOX
   Murine polyomavirus deltaVP1 (NLS deletion mutant)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p414GPD-3xFLAG-Nop4 dE5

   Plasmid

   #169263

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 delta E5 (NOP4 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted region corresponding to human Exon 5

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. p414GPD-3xFLAG-Nop4 dE8

   Plasmid

   #169264

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 delta E8 (NOP4 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted region corresponding to human Exon 8

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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8. p414GPD-3xFLAG-RBM28 dE5

   Plasmid

   #169267

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 delta E5 (RBM28 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted Exon 5

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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9. p414GPD-3xFLAG-RBM28 dE8

   Plasmid

   #169268

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 delta E8 (RBM28 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted Exon 8

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO - RBM28 E5 GT>A minigene

    Plasmid

    #169270

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 5 GT>A minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 E5 GT>A minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NM_018077.2:c.(541+1_541+2delinsA)

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO - RBM28 E8 WT minigene

    Plasmid

    #169271

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 E8 WT minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV_3XHA-ATP6V1B2-mNeonGreen

    Plasmid

    #228865

    Purpose

    Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V1B2 (ATP6V1B2 Human)
    

    Use

    Lentiviral

    Tags

    3xHA and mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_3xFLAG-ATP6V0A3-mScarlet

    Plasmid

    #228867

    Purpose

    Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

    Depositor

    Wilhelm Palm

    Article

    Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

    Insert

    ATP6V0A3 (TCIRG1 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG and mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-ERT2-iCre-ERT2

    Plasmid

    #178059

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin 1 (Syn) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMMK ENTR 1 CMV EYFP Tubulin

    Plasmid

    #206254

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    EYFP Tubulin
    

    Use

    Multimate/gateway entr 1

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMMK ENTR 2 CMV mTFP1 Actin

    Plasmid

    #206255

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mTFP1 Actin
    

    Use

    Multimate/gateway entr 2

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMMK ENTR 3 CMV mito mCherry

    Plasmid

    #206256

    Purpose

    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mito mCherry
    

    Use

    Multimate/gateway entr 3

    Tags

    COX8 mitochondrial tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC19-E3CY56

    Plasmid

    #103075

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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