We narrowed to 1,029 results for: gibson assembly

1. p414GPD-3xFLAG-Nop4 dE5

   Plasmid

   #169263

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 delta E5 (NOP4 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted region corresponding to human Exon 5

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. p414GPD-3xFLAG-Nop4 dE8

   Plasmid

   #169264

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   Nop4 delta E8 (NOP4 Budding Yeast)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted region corresponding to human Exon 8

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. p414GPD-3xFLAG-RBM28 dE5

   Plasmid

   #169267

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 delta E5 (RBM28 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted Exon 5

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. p414GPD-3xFLAG-RBM28 dE8

   Plasmid

   #169268

   Purpose

   S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 delta E8 (RBM28 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Yeast

   Mutation

   deleted Exon 8

   Promoter

   GPD

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5/FRT/TO - RBM28 E5 GT>A minigene

   Plasmid

   #169270

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 5 GT>A minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E5 GT>A minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NM_018077.2:c.(541+1_541+2delinsA)

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5/FRT/TO - RBM28 E8 WT minigene

   Plasmid

   #169271

   Purpose

   Mammalian vector for constitutive expression of RBM28 Exon 8 WT minigene

   Depositor

   Susan Baserga

   Article

   Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

   Insert

   RBM28 E8 WT minigene (RBM28 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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7. plentiCRISPR-sgMDH1

   Plasmid

   #72875

   Purpose

   The lentiviral sgMDH1 vectors was generated via ligation of hybridized oligos (below) into lentiCRISPR-v1 vector linearized with BsmBI using Gibson assembly (NEB

   Depositor

   David Sabatini

   Article

   Birsoy et al Cell. 2015 Jul 30;162(3):540-51. doi: 10

   Insert

   sgMDH1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 4, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pLV_3XHA-ATP6V1B2-mNeonGreen

   Plasmid

   #228865

   Purpose

   Stable expression of V-ATPase subunit ATP6V1B2 with N-terminal 3xHA and C-terminal mNeonGreen

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V1B2 (ATP6V1B2 Human)
   

   Use

   Lentiviral

   Tags

   3xHA and mNeonGreen

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLV_3xFLAG-ATP6V0A3-mScarlet

   Plasmid

   #228867

   Purpose

   Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

   Depositor

   Wilhelm Palm

   Article

   Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

   Insert

   ATP6V0A3 (TCIRG1 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG and mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-Syn-ERT2-iCre-ERT2

    Plasmid

    #178059

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under Syn promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human synapsin 1 (Syn) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-UBC-ERT2-iCre-ERT2

    Plasmid

    #178060

    Purpose

    Conditionally active form of Cre recombinase (activated in response to tamoxifen) under UBC promoter.

    Depositor

    Edward Boyden

    Article

    Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ERT2-iCre-ERT2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human ubiquitin C (UBC) promoter

    Available since

    Oct. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMMK ENTR 1 CMV EYFP Tubulin

    Plasmid

    #206254

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes EYFP Tubulin under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    EYFP Tubulin
    

    Use

    Multimate/gateway entr 1

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMK ENTR 2 CMV mTFP1 Actin

    Plasmid

    #206255

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mTFP1 Actin
    

    Use

    Multimate/gateway entr 2

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMMK ENTR 3 CMV mito mCherry

    Plasmid

    #206256

    Purpose

    ENTR Vector 3 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mitochondrially localised mCherry under the control of CMV promoter.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mito mCherry
    

    Use

    Multimate/gateway entr 3

    Tags

    COX8 mitochondrial tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMDS DEST

    Plasmid

    #206264

    Purpose

    DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. After LR propagate in Pir+. Works as a CRE-donor in the MultiBac system.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Type

    Empty backbone

    Use

    Cre-donor, multimate/gateway dest vector (multiba…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC19-E3CY56

    Plasmid

    #103075

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E3CY56(Cas9 coding gene from Aminomonas paucivorans DSM 12260)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUC19-E6MBW6

    Plasmid

    #103102

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    E6MBW6(Cas9 coding gene from Staphylococcus lugdunensis M23590)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUC19-G2ZYP2

    Plasmid

    #103110

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    G2ZYP2(Cas9 coding gene from Ralstonia syzygii R24)
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUC19-Q927P4

    Plasmid

    #103131

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q927P4(Cas9 coding gene from Listeria innocua serovar 6a (strain CLIP 11262))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCAG-B2UP49_GFP

    Plasmid

    #103071

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage, also expresses EGFP

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    B2UP49(Cas9 coding gene from Akkermansia muciniphila (strain ATCC BAA-835))
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge