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pTRE3G-HA-human cGAS

Plasmid

#186851

Purpose

Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

Insert

Human cGAS with N-terminal HA tag (Adamts1 Human)

Use

Retroviral

Tags

HA

Promoter

TRE3G

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

Plasmid

#223145

Purpose

Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

Plasmid

#223155

Purpose

Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

MBD2 (MBD2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 176-231

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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JO-pH720-SARS-CoV-2-S-tail-WT

Plasmid

#218453

Purpose

Bacterial expression plasmid for the cytosolic tail of SARS-CoV-2 spike with an N-terminal eXact tag

Depositor

Syed Saif Hasan

Article

Dey et al Nat Commun. 2023 Dec 15;14(1):8358. doi:

Insert

Cytosolic tail of SARS-CoV-2 spike carrying an N-terminal eXact tag (S SARS-CoV-2)

Tags

eXact

Expression

Bacterial

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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SpyTag-RBD Omicron BQ.1.1

Plasmid

#214725

Purpose

Expresses SARS-CoV-2 Omicron BQ.1.1 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

Depositor

Mark Howarth

Article

Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

Insert

Receptor-Binding Domain (S SARS-CoV-2)

Tags

SpyTag

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pSLQ7578 pHR (hU6-crPROX-EFS-PuroR-WPRE)

Plasmid

#214882

Purpose

Lentiviral vector encoding RfxCas13d targeting PROX guide array

Depositor

Stanley Qi

Article

Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

Insert

hU6-crPROX-EFS-PuroR-WPRE

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

April 4, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CFAP52:1-620

Plasmid

#211235

Purpose

Mammalian expression of full-length human CFAP52. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CFAP52:M1-S620 (WDR16 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

A91V C199S E336K annotated sequence conflicts

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW10:1-1061

Plasmid

#211207

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_CFAP52:1-620

Plasmid

#211309

Purpose

Mammalian expression of full-length human CFAP52. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CFAP52:M1-S620 (WDR16 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

A91V C199S E336K annotated sequence conflicts

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_FBXW10:1-1061

Plasmid

#211281

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 W391A pcDNA3

Plasmid

#206085

Purpose

expression of rabbit Cav2.2 calcium channel with a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Leroy et al J Neurosci. 2005 Jul 27;25(30):6984-96.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Palpha6 and Interface-4 (a)

Plasmid

#204434

Purpose

Mammalian expression plasmid of GFP-tagged hOpa1 isoform 1 mutant protein.

Depositor

Keir Neuman

Article

Nyenhuis et al Nature. 2023 Aug 23. doi: 10.1038/s41586

Insert

hOpa1 isoform 1 (OPA1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

F860A Y861A Y862A Y863A Q864A R865A H866A F867A

Promoter

CMV

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.0-TERT_-100_-79

Plasmid

#193785

Purpose

luciferase reporter for duplicated TERT promoter

Depositor

Joseph Costello

Article

Barger et al Nat Commun. 2022 Sep 16;13(1):5430. doi:

Insert

TERT promoter (TERT Human)

Use

Luciferase

Expression

Mammalian

Promoter

TERT

Available Since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1-nsp7

Plasmid

#201026

Purpose

Expresses SARS-CoV-2 nsp7 in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Expression

Mammalian

Promoter

T7 promoter

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-nsp7

Plasmid

#201025

Purpose

Expresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Expression

Mammalian

Promoter

CMV-tet-ON

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NvM-DmBam_1-140_AG

Plasmid

#148832

Purpose

Bacterial Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmBam_1-140_AG

Plasmid

#148815

Purpose

Insect Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmBam_1-140_AG

Plasmid

#148820

Purpose

Bacterial Expression of DmBam_1-140

Depositor

Elisa Izaurralde

Article

Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

Insert

DmBam_1-140 (bam Fly)

Expression

Bacterial

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pInducer 21-3xFlag-hMEFV-ΔPYD

Plasmid

#195478

Purpose

pInducer21 plasmid containing the human MEFV gene with a deletion of residues 1 - 92 (the resulting pyrin protein lacks the PYD domain) with a 3xFlag tag at the N-terminus

Depositor

Thomas Henry

Article

Chirita et al Cell Death Dis. 2023 Mar 25;14(3):213. d

Insert

MEFV (MEFV Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

deletion of residues 1-92 of pyrin (the PYD domai…

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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