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Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

Plasmid

#229789

Purpose

Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF10 and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pGL4_10xUAS-CMVmin-BC0396-luc2

Plasmid

#227115

Purpose

Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene

Expression

Mammalian

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

Plasmid

#227245

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_WDR62:1-1518

Plasmid

#211257

Purpose

Mammalian expression of full-length human WDR62 isoform 4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

WDR62:M1-H1518 (WDR62 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 4. L850S natural VAR_031299. Q1305L n…

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-GABA-A-R-Pi [N431/64R]

Plasmid

#206576

Purpose

Mammalian Expression Plasmid of anti-GABA-A-R-Pi (Human). Derived from hybridoma N431/64.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA-A-R-Pi (Homo sapiens) recombinant Mouse monoclonal antibody (GABRP Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Kv1.4 K+ channel (extracellular) scFv [L71/5]

Plasmid

#190517

Purpose

Mammalian Expression of Kv1.4 K+ channel (extracellular) scFV. Derived from hybridoma L71/5.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kv1.4 K+ channel (extracellular) (Rattus norvegicus) recombinant scFV (Kcna4 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV3 [N15/39R]

Plasmid

#188210

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/39

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-MFF [N382/14]

Plasmid

#190318

Purpose

Mammalian Expression Plasmid of anti-MFF (Human). Derived from hybridoma N382/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-MFF (Homo sapiens) recombinant Mouse monoclonal antibody (MFF Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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GluN2B/NR2B glutamate receptor scFv [N59/20]

Plasmid

#190562

Purpose

Mammalian Expression of GluN2B/NR2B glutamate receptor scFV. Derived from hybridoma N59/20.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

GluN2B/NR2B glutamate receptor (Rattus norvegicus) recombinant scFV (Grin2b Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-VAChT [N6/38R]

Plasmid

#182117

Purpose

Mammalian Expression Plasmid of anti-VAChT (Human). Derived from hybridoma N6/38.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-VAChT (Homo sapiens) recombinant mouse monoclonal antibody (SLC18A3 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Tiam1 [L130/1R]

Plasmid

#177476

Purpose

Mammalian Expression Plasmid of anti-Tiam1 (Mouse). Derived from hybridoma L130/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Tiam1 (Mus musculus) recombinant mouse monoclonal antibody (Tiam1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Anti-Collybistin [L120/12R]

Plasmid

#177575

Purpose

Mammalian Expression Plasmid of anti-Collybistin (Rat). Derived from hybridoma L120/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Collybistin (Rattus norvegicus) recombinant mouse monoclonal antibody (Arhgef9 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-NEK9W967A

Plasmid

#168271

Purpose

Expresses LIR (LC3-interacting region) mutant NEK9

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

NEK9 (NEK9 Human)

Tags

FLAG

Expression

Mammalian

Mutation

changed tryptophan 979 to alanine

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

Plasmid

#160628

Purpose

Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos, 35S, 35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBKWH-Lam

Plasmid

#133899

Purpose

Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

Depositor

Sebastian Maurer

Article

Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

Insert

LMNA (Lam) (LMNA Human)

Expression

Yeast

Promoter

T7

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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pSB63 - pL0_fLUC-I (CDS1)

Plasmid

#123180

Purpose

Golden Gate (MoClo; CDS1) compatible luciferase gene from Photinuspyralis with an intron for transient and stable expression in plants

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

Luciferase gene from Photinuspyralis with intron from U5 small nuclear ribonucleoprotein component (Arabidopsis thaliana)

Use

Luciferase; Part for plant expression

Expression

Plant

Mutation

No BpiI and BsaI sites

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 SIRT1-deltaExon2

Plasmid

#105671

Purpose

Mammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)

Depositor

Ullas Kolthur-Seetharam

Article

Deota et al Cell Rep. 2017 Mar 28;18(13):3069-3077.

Insert

SIRT1 (Sirt1 Mouse)

Expression

Mammalian

Mutation

Synonymous base changes not affecting the protein…

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pVENUS-N1-FNTA

Plasmid

#86859

Purpose

To express FNTA with a Venus tag at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

FNTA (FNTA Human)

Tags

Venus

Expression

Mammalian

Mutation

The sequence of the insert is isoform 2, which di…

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pZC14 (pFA6a-PSc-6xGly-V5-HphMX)

Plasmid

#44079

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

6xGly linker, ADH1 transcription terminator, HphM…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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pZC03 (pFA6a-TEV-6xGly-V5-His3MX)

Plasmid

#44072

Depositor

Tim Formosa

Type

Empty backbone

Use

Pcr-based yeast c-terminal tagging

Tags

6xGly linker, ADH1 transcription terminator, His3…

Available Since

April 2, 2013

Availability

Academic Institutions and Nonprofits only

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