We narrowed to 15,187 results for: pET

1. pEGFP-PCNA-IRES-puro2b

   Plasmid

   #26461

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   PCNA (PCNA Human)
   

   Tags

   pEGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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2. gWiz_BM40-vGAT-VL-hIgG1-CL

   Plasmid

   #218437

   Purpose

   mammalian expression plasmid for the light chain fragment of an anti-vGAT Fab domain

   Depositor

   Axel Brunger

   Article

   Gopal et al Curr Res Physiol. 2024 Feb 23;7:100121.

   Insert

   vGAT-VL-hIgG1-CL
   

   Use

   Affinity Reagent/ Antibody

   Tags

   BM40

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-EFS-PCP-3xSNAP-NLS

   Plasmid

   #236388

   Purpose

   Overexpression of PCP-3xSNAP in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

   Insert

   PCP-3xSNAP
   

   Use

   Lentiviral

   Available Since

   July 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSNRW-Z5-Nluc

   Plasmid

   #174487

   Purpose

   Recombinant fusion protein gene combining IgG-Fc binding domain Z (5 repeats) of Staphylococcal Protein A and bioluminescence reporter Nanoluciferase in pET28a bacterial expression plasmid.

   Depositor

   Richard C. Willson

   Article

   Nandy et al Anal Biochem. 2022 Oct 18:114929. doi: 1

   Insert

   Z5-Nluc
   

   Tags

   6x Histidine tag (N terminal on insert)

   Expression

   Bacterial

   Promoter

   T7lac promoter

   Available Since

   Oct. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-FLAG-SBP-STAG2 (WT)

   Plasmid

   #73963

   Purpose

   STAG2 cDNA (CCDS43990) wild type

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   wild type

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

6. pCALNL-NaChBac

   Plasmid

   #125580

   Purpose

   Cre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.

   Depositor

   Takeshi Imai

   Article

   Fujimoto et al Dev Cell. 2023 Jul 24;58(14):1221-1236.e

   Insert

   NaChBac
   

   Use

   Cre/Lox

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   July 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSynapsin1-axon-GCaMP6s

   Plasmid

   #111262

   Purpose

   For enriched expression of GCaMP6s in axons

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV1

   Insert

   axon-GCaMP6s
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Promoter

   hSynapsin1

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCX-SpiCee-NLS

   Plasmid

   #140900

   Purpose

   mammalian expression of SpiCee-NLS, a nucleus-restricted variant of the calcium buffer SpiCee that prevents downstream signaling

   Depositor

   Xavier Nicol

   Article

   Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

   Insert

   SpiCee-NLS
   

   Tags

   mRFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. His-AgdNK

   Plasmid

   #203226

   Purpose

   For protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
   

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-mApple-6xHp

    Plasmid

    #217376

    Purpose

    Lipid droplet localization of 6xHp of Spastin fused to mApple

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    mApple-6x Hp NC
    

    Use

    Lentiviral

    Tags

    mApple

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. NFAST(low)-mApple-mito

    Plasmid

    #214415

    Purpose

    Mitochondria localization of low-affinity NFAST fused to mApple-mito

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    RspA-N
    

    Tags

    mApple-mito

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCIB1(deltaNLS)-pmGFP

    Plasmid

    #28240

    Purpose

    Expresses CIB1 fused to plasma membrane-localized EGFP

    Depositor

    Chandra Tucker

    Article

    Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

    Insert

    CIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    NLS sequences mutated

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. FAB(ER-LD)

    Plasmid

    #214424

    Purpose

    Detection of ER-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    FRB-V5
    

    Tags

    CFAST-Halo and RspAN-FRB-V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. hDHFR

    Plasmid

    #184707

    Purpose

    bacterial expression of human DHFR

    Depositor

    Peter Wright

    Article

    Bhabha et al FEBS Lett. 2011 Nov 16;585(22):3528-32.

    Insert

    DHFR (DHFR Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. wt dynamin 1 pEGFP

    Plasmid

    #34680

    Depositor

    Sandra Schmid

    Article

    Song et al Mol Biol Cell. 2004 May;15(5):2243-52. E

    Insert

    dynamin 1ba (DNM1L Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pCX-SpiCee-NES

    Plasmid

    #140901

    Purpose

    mammalian expression of SpiCee-NES, a nucleus-excluded variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    SpiCee-NES
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPB-TRE-ECC-Biosensor

    Plasmid

    #140833

    Purpose

    Encoding the TRE promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    TRE-ECC Biosensor
    

    Use

    Piggybac (pb) transposon

    Expression

    Mammalian

    Promoter

    inverse

    Available Since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

    Plasmid

    #170373

    Purpose

    Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCX-NTOM-SpiCee

    Plasmid

    #140902

    Purpose

    mammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2020 Jul 21;32(3):107934. doi:

    Insert

    NTOM-SpiCee
    

    Tags

    mRFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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