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POC1423

Plasmid

#221559

Purpose

Plasmid designed to assay the site-selective methylation protection approach using the non-switchable methylases

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Type

Empty backbone

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1426

Plasmid

#221573

Purpose

Plasmid containing the Insert 1 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1427

Plasmid

#221574

Purpose

Plasmid containing the Insert 2 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1428

Plasmid

#221575

Purpose

Plasmid containing the Insert 3 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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POC1429

Plasmid

#221576

Purpose

Plasmid containing the Insert 4 to be assembled in POC1430 using the site-selective methylation protection approach

Depositor

Christopher A. O'Callaghan

Publication

Flores-Fernandez et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DNA fragment flanked by methylation-protectable BsaI sites and containing one always-methylable internal BsaI site

Use

Synthetic Biology

Available since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGH916

Plasmid

#230842

Purpose

Ef1a-DivA-BE3-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE3

Use

Lentiviral

Expression

Mammalian

Available since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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REV7 C-terminal sgRNA

Plasmid

#207095

Purpose

pX330 based plasmid for expression of Cas9 and the GCTCATAAAGGCAGCTGAGG sgRNA to target the REV7 locus.

Depositor

Jens Schmidt

Publication

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GCTCATAAAGGCAGCTGAGG

Expression

Mammalian

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pVEA3-delta7

Plasmid

#234802

Purpose

Mammalian expression of bovine arrestin-3 (deleted residues 176, 177, 178, 182-185)

Depositor

Vsevolod Gurevich

Publication

Zheng et al Cells. 2023 Jun 6;12(12):1563. doi: 10.3

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

deleted residues 176, 177, 178, 182-185

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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NarP-GFP

Plasmid

#220161

Purpose

To track the natural NarP promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Publication

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

NarP promoter only

Expression

Bacterial

Available since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGH907

Plasmid

#230837

Purpose

Ef1a-MS2-AIDDead-T2A-GFP-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AIDDead

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH518

Plasmid

#230836

Purpose

Ef1a-MS2-AID*-T2A-GFP-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

MS2-AID*

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pGH896

Plasmid

#230840

Purpose

Ef1a-DivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH897

Plasmid

#230841

Purpose

Ef1a-nDivA-BE-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

nDivA-BE

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH811

Plasmid

#230850

Purpose

Ef1a-nDivA-BE-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

nDivA-BE

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH720

Plasmid

#230845

Purpose

Ef1a-ABE7.10-T2A-tagBFP-wPRE

Depositor

Gaelen Hess

Publication

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

ABE7.10

Use

Lentiviral

Expression

Mammalian

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371D

Plasmid

#224417

Purpose

Mammalian expression of KIF 13a S1371D

Depositor

Marvin Bentley

Publication

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371D (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371D

Promoter

Chicken Beta actin

Available since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCKC017

Plasmid

#226622

Purpose

pLenti wt hTdT template for libraries 1-2

Depositor

Chang Liu

Publication

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Mutation

synonymous mutation in D2, synonymous mutation in…

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.FRB-3myc-KIF13AWLDLE tail 361-1749

Plasmid

#224412

Purpose

Mammalian expression of KIF 13a WLDLE

Depositor

Marvin Bentley

Publication

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a WLDLE (Kif13a Mouse)

Tags

FRB-3myc

Expression

Mammalian

Mutation

WLDLE

Promoter

Chicken Beta actin

Available since

Oct. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.GFP-KIF13AS1371A

Plasmid

#224409

Purpose

Mammalian expression of KIF 13a S1371A

Depositor

Marvin Bentley

Publication

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

KIF 13a S1371A (Kif13a Mouse)

Tags

GFP

Expression

Mammalian

Mutation

S1371A

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pBa.KIF13A

Plasmid

#224408

Purpose

Mammalian expression of Kif 13a

Depositor

Marvin Bentley

Publication

Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

Insert

Kif 13a (Kif13a Mouse)

Expression

Mammalian

Promoter

Chicken Beta actin

Available since

Sept. 20, 2024

Availability

Academic Institutions and Nonprofits only

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