We narrowed to 15,991 results for: LEA

1. C31m

   Plasmid

   #120966

   Purpose

   MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 10.1007/s00438-006-0129-5. Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   bxb1 integrase
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. C11m

   Plasmid

   #120959

   Purpose

   MoClo golden gate assembly CD part for Citrine (modified YFP; variant of https://www.fpbase.org/protein/citrine/) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   Citrine
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. C115m

   Plasmid

   #121014

   Purpose

   MoClo golden gate assembly CD part for TP901 integrase (Integrase from lactococcal phage TP901). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   TP901 integrase
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMLLAC-J72041

   Plasmid

   #26186

   Depositor

   Christopher Anderson

   Insert

   Tev Protease Cleavage site
   

   Use

   Synthetic Biology; 2ab assembly vector

   Available Since

   June 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTcTurboID-GW_GFP-NLS

   Plasmid

   #234716

   Purpose

   Used to express GFP-3xNLS fused to 3xHA-TurboID (N-terminal) in T. cruzi. Tetracycline regulated. Used as proximity labelling nuclear spatial control.

   Depositor

   Esteban Serra

   Insert

   GFP-3xNLS
   

   Use

   Trypanosomatid expression

   Tags

   3xHA

   Promoter

   T7

   Available Since

   May 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. C84m

   Plasmid

   #120995

   Purpose

   MoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   Sigma 28
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. C40m

   Plasmid

   #120973

   Purpose

   MoClo golden gate assembly CD part for blank part (random 50bp sequence) - Please see Supplemental Documents for annotated Genbank file

   Depositor

   Richard Murray

   Insert

   Blank (Random 50bp sequence)
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. C59m

   Plasmid

   #120976

   Purpose

   MoClo golden gate assembly CD part for mScarlet (RFP variant; see https://www.fpbase.org/protein/mscarlet/). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   mScarlet
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. C63m

   Plasmid

   #120979

   Purpose

   MoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.

   Depositor

   Richard Murray

   Insert

   lacI
   

   Use

   Synthetic Biology

   Available Since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. P30m

    Plasmid

    #120942

    Purpose

    MoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.

    Depositor

    Richard Murray

    Insert

    pL-lacO1
    

    Use

    Synthetic Biology

    Available Since

    Dec. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-NFIX2ERT2

    Plasmid

    #112704

    Purpose

    Expression of NFIX2-ERT2 fusion protein

    Depositor

    Richard Gronostajski

    Insert

    Nuclear Factor I X2 (Nfix Mouse)
    

    Tags

    ERT2 and HA

    Expression

    Mammalian

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. HA-CPI-17-T38A-NLS

    Plasmid

    #100780

    Purpose

    Mammalian expression of CPI-17 neutral mutant nuclear

    Depositor

    Joseph Gordon

    Insert

    CPI-17-T38A-NLS (PPP1R14A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T38A

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-CPI-17-T38E-NLS

    Plasmid

    #100778

    Purpose

    Mammalian expression of CPI-17 phospho-mimetic nuclear

    Depositor

    Joseph Gordon

    Insert

    CPI-17-T38E-NLS (PPP1R14A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T38E

    Promoter

    CMV

    Available Since

    Sept. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. NET29B.mm pRRLSIN (1744)

    Plasmid

    #62053

    Purpose

    lentiviral expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Insert

    NET29B (Tmem120b Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET GFP LIC cloning vector (2GFP-T)

    Plasmid

    #29716

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    6xHis, SiriusGFP, and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOpen-SfiIR (cDNA)

    Plasmid

    #165528

    Purpose

    Type II restriction enzyme that recognizes the double-stranded sequence GGCCNNNNNGGCC and cleaves after N-4.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    SfiI (used to create cDNA libraries)
    

    Use

    Synthetic Biology

    Available Since

    Aug. 5, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pExp-NusA

    Plasmid

    #112571

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8His and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-MHL

    Plasmid

    #26096

    Purpose

    SGC Empty backbone for bacterial expression

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Tags

    6xHis and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7-lacO (lactose/IPTG inducible)

    Available Since

    Aug. 20, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pExp-NHis

    Plasmid

    #112558

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGG-NFIC2

    Plasmid

    #112701

    Purpose

    Expression of NFIC2 protein and GFP

    Depositor

    Richard Gronostajski

    Insert

    Nuclear Factor I C2 (Nfic Mouse)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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