Deciphering RNA splicing logic with interpretable machine learning.
Liao SE, Sudarshan M, Regev O
Proc Natl Acad Sci U S A. 2023 Oct 10;120(41):e2221165120. doi: 10.1073/pnas.2221165120. Epub 2023 Oct 5. PubMed Article
Liao SE, Sudarshan M, Regev O
Proc Natl Acad Sci U S A. 2023 Oct 10;120(41):e2221165120. doi: 10.1073/pnas.2221165120. Epub 2023 Oct 5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 204143 | pRegev_V1 | Single reporter randomly selected from library for biochemical validation |
| 204144 | pRegev_V2 | Single reporter randomly selected from splicing library for biochemical validation. |
| 204145 | pRegev_V3 | Single reporter randomly selected from splicing library for biochemical validation |
| 204150 | pRegev_S1 | Single reporter with secondary structure from splicing library. |
| 204160 | pRegev_S1.46G>U | pRegev_S1 with single mutation that disrupts secondary structure. |
| 204161 | pRegev_S1.34C>A_46G>U | pRegev_S1 with double mutations that restore secondary structure. |
| 204162 | pRegev_S2 | Single reporter with secondary structure from splicing library. |
| 204163 | pRegev_S2.39U>G | pRegev_S2 with single mutation that disrupts secondary structure. |
| 204165 | pRegev_S2.39U>G.51A>C | pRegev_S2 with double mutations that restore secondary structure. |
| 204170 | pRegev_S4 | Single reporter with secondary structure from splicing library. |
| 204171 | pRegev_S4.39U>G | pRegev_S4 with single mutation that disrupts secondary structure. |
| 204172 | pRegev_S4.51A>C | pRegev_S4 with single mutation that disrupts secondary structure. |
| 204173 | pRegev_S4.39U>G.51A>C | pRegev_S4 with double mutations that restore secondary structure. |
| 204174 | pRegev_S5 | Single reporter with secondary structure from splicing library. |
| 204175 | pRegev_S5.49C>A | pRegev_S5 with single mutation that disrupts secondary structure. |
| 204177 | pRegev_S5.49C>A.59G>U | pRegev_S5 with double mutations that restore secondary structure. |
| 204178 | pRegev_D1 | Single reporter with long guanine depleted (G-poor) region from splicing library. |
| 204179 | pRegev_D1.61C>G | pRegev_D1 with single C>G mutation. |
| 204180 | pRegev_D2 | Single reporter with long guanine depleted (G-poor) region from splicing library. |
| 204181 | pRegev_D2.30C>G | pRegev_D2 with single C>G mutation. |
| 204182 | pRegev_D3 | Single reporter with long guanine depleted (G-poor) region from splicing library. |
| 204183 | pRegev_D3.48C>G | pRegev_D3 with single C>G mutation. |
| 204184 | pRegev_D4 | Single reporter with long guanine depleted (G-poor) region from splicing library. |
| 204185 | pRegev_D4.34C>G | pRegev_D4 with single C>G mutation. |
| 204186 | pRegev_ExonSkip_LandingPad | Backbone for cloning individual exon sequences into beta globin minigene splicing reporter. |
| 204187 | pRegev_ExonSkip_Backbone | Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1). |