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pGEX-4T-3-SH3BP1-CTD

Plasmid

#136649

Purpose

Expression of SH3BP1-CTD-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-CTD (SH3BP1 Human)

Expression

Bacterial

Mutation

Contains only C-terminal domain

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1-NTD

Plasmid

#136648

Purpose

Expression of SH3BP1-NTD-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1-NTD (SH3BP1 Human)

Expression

Bacterial

Mutation

Contains only N-terminal domain

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPR_eGFP_314

Plasmid

#111822

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 294-314

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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DIRAS3

Plasmid

#55686

Purpose

expression of human DIRAS3

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

DIRAS3 (DIRAS3 Human)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pT3TS-Dest_R1-R3

Plasmid

#140878

Purpose

166571: Multisite gateway destination vector for in vitro transcription of RNA using T3. Accepts one pME and one p3E entry clone.

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

ccdb/CAM

Expression

Mammalian

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV Puro-EFS:GFP

Plasmid

#68484

Purpose

Retrovirus expressing GFP driven by EFS promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Puromycin Resistance
GFP

Use

Retroviral

Expression

Mammalian

Promoter

EFS and LTR

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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human b1 ecto-pIRES

Plasmid

#113387

Purpose

Express human beta 1 ectodomain with secretion peptide, purification tags, and C-terminal clasp

Depositor

Timothy Springer

Article

Li et al EMBO J. 2017 Mar 1;36(5):629-645. doi: 1

Insert

human beta 1 ectodomain

Tags

His tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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Pr-deGFP-MGapt

Plasmid

#67734

Purpose

OR2-OR1-Pr promoter expressing deGFP-MG aptamer fusion

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-deGFP-MGapt-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH1

Plasmid

#168977

Purpose

AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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Flag-FOXN4

Plasmid

#153140

Purpose

Expression of Flag tagged human FOXN4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXN4 (FOXN4 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pME-mKOFP2-CAAX

Plasmid

#75155

Purpose

Membrane targeted KOFP2 (orange) fluorophore for use with 5’ promoters.

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

mKOFP2-CAAX

Use

Middle entry vector for multisite gateway three f…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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JC101: pMVP/Ad/PK-DEST

Plasmid

#121845

Purpose

pMVP destination vector, empty pK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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actc1b-mKate2-CaaX(tH)

Plasmid

#109487

Purpose

Muscle specific zebrafish expression of mKate2 fused to the CaaX domain from Hras

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

actc1b-mKate2-CaaX(tH)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-Wnt2-V5

Plasmid

#35925

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Tags

V5

Expression

Mammalian

Mutation

Silent Mutation G (816) --> A

Promoter

CMV

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pENTR221-Firefly

Plasmid

#79495

Purpose

MultiSite Gateway entry clones, second fragment (attL5, attL2) Firefly Luciferase CDS

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

Firefly Luciferase

Use

Multisite gateway entry clone

Promoter

no Promoter

Available Since

May 12, 2017

Availability

Academic Institutions and Nonprofits only

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TOPO CCSP

Plasmid

#68453

Purpose

TOPO construct expressing CCSP for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Clara Cell Secretory Protein Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::PuroR-bGHpA

Plasmid

#62440

Purpose

MXS_chaining vector with PGK::PuroR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with PGK Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::NeoR-bGHpA

Plasmid

#62437

Purpose

MXS_chaining vector with CMV::NeoR-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Neomycin with CMV Promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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TOPO SV40

Plasmid

#68455

Purpose

TOPO construct expressing SV40 for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Simian Virus Early 40 Promoter

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAGM7547

Plasmid

#169091

Purpose

MoClo level 1 35S promoter-Db5GT-35S terminator transcription unit

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

Insert

35S promoter-Db5GT-35S terminator

Use

Moclo cloning vector

Expression

Plant

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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