We narrowed to 10,185 results for: yeast

1. pGal4AD-CIBN

   Plasmid

   #28246

   Purpose

   Expresses Gal4 Activation Domain fused to CIBN (aa1-170) for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   CIB1 (1-171aa)
   

   Tags

   Gal4AD

   Expression

   Yeast

   Available Since

   March 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPMVAu8

   Plasmid

   #98297

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
   

   Expression

   Yeast

   Available Since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENO1-dTom-NATr

   Plasmid

   #52208

   Purpose

   Constitutive expression of Candida codon-optimized dTomato with NAT selectable marker

   Depositor

   Robert Wheeler

   Article

   Gratacap et al Dis Model Mech. 2013 Sep;6(5):1260-70. d

   Insert

   dTomato
   

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for Candida species

   Promoter

   ENO1

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

4. POM43-mTFP1

   Plasmid

   #69853

   Purpose

   N-terminal tagging with mTFP1 using LEU2 as a selection marker in S. cerevisiae.

   Depositor

   Liza Pon

   Article

   Higuchi-Sanabria et al PLoS One. 2016 Jan 4;11(1):e0146120. doi

   Insert

   mTFP1 minus start and stop codon
   

   Expression

   Yeast

   Available Since

   April 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. eGFP L202 gRNA

   Plasmid

   #119132

   Purpose

   gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

   Depositor

   Reuben Harris

   Article

   Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

   Insert

   eGFP L202 gRNA
   

   Use

   CRISPR

   Mutation

   None

   Promoter

   U6

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJJB1561

   Plasmid

   #218626

   Purpose

   Express geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimer

   Depositor

   John Dueber

   Article

   Baker et al Nat Chem Biol. 2024 Oct 14. doi: 10.1038

   Insert

   GES
   

   Tags

   ePTS1

   Expression

   Yeast

   Available Since

   Oct. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pWS931

   Plasmid

   #123053

   Purpose

   TetR bacterial repressor

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   TetR-NLS [3a]
   

   Expression

   Yeast

   Available Since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWS1806

   Plasmid

   #123047

   Purpose

   Melatonin GPCR

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   MTNR1A [3]
   

   Expression

   Yeast

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUNIV-EGFP

   Plasmid

   #24706

   Depositor

   Cynthia Czajkowski

   Article

   Venkatachalan et al Pflugers Arch. 2007 Apr . 454(1):155-63.

   Insert

   EGFP (eGFP Aequoria victoria)
   

   Expression

   Bacterial, Mammalian, and Y…

   Available Since

   Dec. 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWS625

    Plasmid

    #123046

    Purpose

    Adenosine GPCR

    Depositor

    Tom Ellis

    Article

    Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

    Insert

    A2BR [3]
    

    Expression

    Yeast

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRG706_lexO-SbfI_LexA-TF_LEU2MX

    Plasmid

    #154818

    Purpose

    Inducible expression of SbfI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    SbfI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGY53

    Plasmid

    #221709

    Purpose

    Empty Cas12a vector for multiplex gene editing in fungi

    Depositor

    Kyle Pomraning

    Article

    Yuan et al Front Bioeng Biotechnol. 2024 Oct 16;12:

    Type

    Empty backbone

    Use

    CRISPR; Aspergillus

    Expression

    Bacterial

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMVTnT-Flp-3xFLAG

    Plasmid

    #109167

    Purpose

    Encodes yeast full-length Flp with C-terminal 3xFLAG tag to be expressed in a mammalian expression system

    Depositor

    Monika Golas

    Article

    Jensen et al Protein J. 2017 Aug;36(4):332-342. doi:

    Insert

    Flp
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRG659_lexO-FseI_LexA-TF_LEU2MX

    Plasmid

    #154816

    Purpose

    Inducible expression of FseI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    FseI endonuclease
    LexA-ER-B112
    

    Tags

    FLAG and NLS

    Expression

    Yeast

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDIS3

    Plasmid

    #92173

    Purpose

    Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by disruption of the region targeted for recombination.

    Depositor

    Judith Berman

    Article

    Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

    Insert

    NAT1
    

    Use

    Genbank: u03440.1

    Expression

    Yeast

    Available Since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS263

    Plasmid

    #122379

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. eGFP L138 gRNA

    Plasmid

    #119133

    Purpose

    gRNA that guides Cas9 and APOBEC complexes to L138 in eGFP.

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    eGFP L138 gRNA
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    U6

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENO1-iRFP-NATr

    Plasmid

    #129731

    Purpose

    Integration plasmid to overexpress iRFP in Candida albicans

    Depositor

    Robert Wheeler

    Article

    Bergeron et al Infect Immun. 2017 Oct 18;85(11). pii: I

    Insert

    iRFP-Candida albicans codon-optimized
    

    Expression

    Yeast

    Promoter

    ENO1 from Candida albicans

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pVG122

    Plasmid

    #211765

    Purpose

    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    strongLOV
    

    Use

    For single-copy integration (if cut with pmei)

    Tags

    SV40 NLS and VP16

    Expression

    Yeast

    Mutation

    compared to EL222, strongLOV contains Glu84Asp mu…

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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