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Plasmid#225243PurposeMammalian expression of iPASnFR-H fused to Rab5DepositorInsertiPASnFR-H
UseTags3xFLAG and mScarlet3ExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-iPASnFR-L
Plasmid#225235PurposeAAV expression of iPASnFR-L from GFAP promoterDepositorInsertiPASnFR-L
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCD86-mEGFP
Plasmid#190745PurposeExpression of mEGFP-tagged CD86 in mammalian cells.DepositorInsertCD86 B7-2, B7.2, B70, CD28LG2, LAB (CD86 Human)
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-MSX1
Plasmid#222557PurposePiggyBac transposon plasmid for doxycycline inducible expression of MSX1DepositorInsertMSX1 (MSX1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-PRDM9
Plasmid#222565PurposePiggyBac transposon plasmid for doxycycline inducible expression of PRDM9DepositorInsertPRDM9 (PRDM9 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
Tol2_8xCTS-ECFP
Plasmid#200249PurposeZebrafish transgenics; Plasmid encodes the sgRNA2_8xCTS-ECFP reporter and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorInsert8xCTS-ECFP
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJAT23
Plasmid#204312PurposeFor generating targeting inversions. C-terminal split GFP, expressed in eyes.DepositorInsert3XP3-GFPc
UseCRISPRTagsExpressionMutationPromoter3XP3Available sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1_CoumRS
Plasmid#64882PurposeExpresses Coumarinyl- tRNA synthetaseDepositorInsertCoum_RS
UseTagsFlagExpressionMutationPromoterCMVAvailable sinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 hNLRP3-mNG-STOP
Plasmid#218644PurposeGateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)DepositorInsertNLRP3 (NLRP3 Human)
UseGateway entryTagsmNeonGreenExpressionMutationPromoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCxcl1-Firefly
Plasmid#163896PurposeLuciferase reporter for mouse Cxcl1DepositorInsertLuciferase
UseLuciferaseTagsExpressionMutationPromoterCxcl1Available sinceJan. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKB225
Plasmid#170015PurposeEncodes LacI-controlled expression of surface display protein with no C-terminal peptide and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Peptide display protein with no C-terminal peptide
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialMutationPromoterPtac and PvirKAvailable sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-lepB
Plasmid#190725Purposefine tune expression of type 1 signal peptidase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-lepB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL0_142 [EilR-PjexD]
Plasmid#199026PurposeLevel 0 partDepositorInsertEilR-PjexD
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMH147
Plasmid#186647PurposeaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1 between homology arms for pilADepositorArticleInsertaadA.co-TaadA-J23107-4ABH.co.rm1-PpsaA-nhoA.co.rm1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSABEd
Plasmid#133968PurposeExpresses ABEd CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEd
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH849-gst-optec
Plasmid#89590PurposePlasmid for bacterial expression of His-tagged GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase codon-optimised for bacterial expression
UseTags8xhistidineExpressionBacterialMutationPromoterT7Available sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSCBE2
Plasmid#133965PurposeExpresses BE2 CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertBE2
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSABEn
Plasmid#133969PurposeExpresses ABEn CRISPR base editor and gRNA scaffold in StreptomycesDepositorArticleInsertABEn
UseCRISPR and Synthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYCTK019
Plasmid#176723PurposeThe plasmid is part of a GG toolkit for cell-cell communication in S. cerevisiae. This plasmid is an L0 part plasmid and contains the Ste2 gene of Candida albicans (receiver part).DepositorArticleInsertste2Ca
UseSynthetic BiologyTagsExpressionMutationCodon-optimized for expression in S. cerevisiaePromoterAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only