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pETNKI-strepII-3C-LIC-kan

Plasmid

#108706

Purpose

Expression of a StrepII-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

StrepII-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pPSP6+t-gfp

Plasmid

#48130

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-SP6-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

Plasmid

#189955

Purpose

shRNA mediated knockdown

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

ERV_MMERVK10c

Use

Lentiviral

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pUT1163-HQ858707

Plasmid

#27753

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from DV507461

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

Plasmid

#86195

Purpose

U6 based expression of N meningitidis sgRNA

Depositor

Scot Wolfe

Article

Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

Insert

nmCas9 sgRNA cloning cassete

Use

Lentiviral

Promoter

U6

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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DsRed +9e

Plasmid

#191766

Purpose

Expression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9e

Depositor

Daniel Gerlich

Article

Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

Insert

DsRed

Tags

N terminal fusion of positively charged polypetod…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pLacI-GFP-HEC1 WT

Plasmid

#114049

Purpose

Expression of exogenous LacI-GFP-HEC1 WT

Depositor

Geert Kops

Article

Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

Insert

GFP-HEC1 (NDC80 Human)

Expression

Mammalian

Mutation

WT HEC1 sequence

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pUT1109-HQ858666

Plasmid

#27719

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

C2C2-CO-like transcription factor from DT643534

Use

Entry vector

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pETNKI-his-3C-LIC-Amp

Plasmid

#108704

Purpose

Expression of a His-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Article

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

His-3C protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pUT1141-HQ858685

Plasmid

#27731

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from EE179215

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1439-HQ858741

Plasmid

#27823

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

NAC transcription factor from DT947867

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1769-HQ858783

Plasmid

#27865

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

DBP transcription factor from EB701836

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-01.ChR2(H134R)-GFP.BCC10

Plasmid

#229639

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-01.ChR2(H134R)-GFP.BCC10

Use

AAV

Expression

Mammalian

Available Since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hRPL3-FLAG_WT

Plasmid

#233616

Purpose

Expression of Flag-tagged hRPL3

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Flag-tagged hRPL3 (RPL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-hRPL3-FLAG_H245A

Plasmid

#233617

Purpose

Expression of Flag-tagged hRPL3 with H245A mutation

Depositor

Shintaro Iwasaki

Article

Matsuura-Suzuki et al Elife. 2022 Jun 8;11. pii: 72780. doi: 1

Insert

Flag-tagged hRPL3 with H245A mutation (RPL3 Human)

Tags

Flag

Expression

Mammalian

Mutation

H245A

Available Since

March 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL5ET-12.EGFP.BCC6.WPRE

Plasmid

#229638

Purpose

L5 extratelencephalic projection neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL5ET-12.EGFP.BCC6

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.RMacL3-05.EGFP.BCB2.WPRE

Plasmid

#229620

Purpose

L3 pyramidal neuron Enhancer to drive transgene expression

Depositor

William Stauffer

Article

He et al Neuron. 2025 May 21;113(10):1548-1561.e8

Insert

RMacL3-05.EGFP.BCB2

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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