We narrowed to 13,205 results for: lic

1. pEEF1D-FLAG (pcDNA 3.1+) LG6-1

   Plasmid

   #37365

   Depositor

   David Litchfield

   Article

   Gyenis et al J Proteome Res. 2011 Nov 4;10(11):4887-9

   Insert

   EEF1D (EEF1D Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 17, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pCAG-hGPR56-IRES-GFP

   Plasmid

   #52297

   Purpose

   Expresses human GPR56 and GFP in mammalian cells.

   Depositor

   Christopher A Walsh

   Article

   Bae et al Science. 2014 Feb 14;343(6172):764-8. do

   Insert

   Human GPR56 coding sequence (ADGRG1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTriEx-mCherry-PA-Rac1-C450A

   Plasmid

   #22028

   Purpose

   Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
   

   Tags

   6xHis and mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMSCVneo-DEST

   Plasmid

   #119746

   Purpose

   Allows Gateway cloning of gene of interest into retroviral pMSCV vector (with Neomycin/G418 resistance)

   Depositor

   Andrew Jackson, Martin Reijns

   Article

   Harley et al Nat Genet. 2016 Jan;48(1):36-43. doi: 10

   Type

   Empty backbone

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV_NESPylRS(AF)_hU6tRNAPyl

   Plasmid

   #223508

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Nikic et al Angew Chem Int Ed Engl. 2016 Dec 23;55(5

   Inserts

   NES Pyrrolysyl-tRNA Synthetase (AF) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Expression

   Mammalian

   Mutation

   Y306A; Y384F

   Promoter

   CVM, SP6 and U6

   Available Since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 (-) hYAP 1-2alpha

   Plasmid

   #124143

   Purpose

   Protein expression for funtional studies of cell growth promotion

   Depositor

   Megan Finch-Edmondson, Marius Sudol

   Article

   Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

   Insert

   YAP1 (YAP1 Human)
   

   Use

   Unspecified

   Mutation

   None (Wild Type)

   Available Since

   April 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. V5-ERG

   Plasmid

   #118623

   Purpose

   Expresses V5 tagged ERG protein

   Depositor

   William Hahn

   Article

   Sandoval et al Mol Cell. 2018 Aug 16;71(4):554-566.e7.

   Insert

   ERG (ERG Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPX8 rescue PLAK1

   Plasmid

   #161517

   Purpose

   Rescues the expression of GPX8 in GPX8 plenti-CRISPR KO cells

   Depositor

   Yoav Shaul

   Article

   Khatib et al Proc Natl Acad Sci U S A. 2020 Sep 1;117

   Insert

   glutathione peroxidase 8 (GPX8 Human)
   

   Use

   Lentiviral

   Tags

   3X Flag

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV HA hRb delta CDK

   Plasmid

   #58906

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   all 15 Ser/Thr Cdk acceptor sites changed to Ala,…

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGM234

    Plasmid

    #220177

    Purpose

    Expresses exogenous wild-type NAC complex members (NACA and BTF3)

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    NACA and BTF3 (NACA, BTF3 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG (NACA); 6xHis (BTF3)

    Available Since

    June 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-GFP-IKBalpha-mut (super repressor)

    Plasmid

    #15264

    Depositor

    William Hahn

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    IKB alpha (NFKBIA Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    Serine 32 to Alanine, Serine 36 to Alanine

    Available Since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-mCherry-PA-Rac1-T17N

    Plasmid

    #22029

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant).

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 (-) hYAP 1-2delta

    Plasmid

    #124146

    Purpose

    Protein expression for funtional studies of cell growth promotion

    Depositor

    Megan Finch-Edmondson, Marius Sudol

    Article

    Gaffney et al Gene. 2012 Nov 10;509(2):215-22. doi: 10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Unspecified

    Mutation

    None (Wild Type)

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDONR223_PKIA_WT

    Plasmid

    #82153

    Purpose

    Gateway Donor vector containing PKIA , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    PKIA (PKIA Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX6P2-p53 (1-90) (72R)

    Plasmid

    #62081

    Purpose

    Expression of human p53 (TAD residues 1-90) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

    Insert

    p53 (TP53 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains TAD/PP residues 1-90, 72R

    Promoter

    tac

    Available Since

    Feb. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

16. peSCBE3-NG

    Plasmid

    #218163

    Purpose

    This plasmid harbors the base editor eSCBE3-NG along with an sgRNA cloning cassette, facilitating high-efficiency cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    escbe3-NG
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutation in the portion of deaminase hAPOBEC3A : …

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-mCerulean-PA-Rac1

    Plasmid

    #22030

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His and mCerulean

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONR223_RHEB_WT

    Plasmid

    #81257

    Purpose

    Gateway Donor vector containing RHEB , part of the Target Accelerator Plasmid Collection.

    Depositor

    Jesse Boehm, William Hahn, David Root

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    RHEB (RHEB Human)
    

    Use

    Gateway entry vector

    Mutation

    WT

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pExp-xMBP-ACE2-CHis

    Plasmid

    #194998

    Purpose

    Production of ACE2 extracellular domain

    Depositor

    Marko Hyvönen

    Article

    Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

    Insert

    ACE2 (ACE2 Human)
    

    Tags

    His8 and xMBP

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSCBE3-NG

    Plasmid

    #218159

    Purpose

    This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / L1111…

    Promoter

    rpsL promoter

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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