We narrowed to 14,953 results for: pET

1. LentiGuide-Neo-CTRLg1

   Plasmid

   #139450

   Purpose

   Lentiviral vector with non-targeting gRNA and neomycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   Non-targeting sgRNA inserted; resistance gene: neoR
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mADAM10 deltaMP in pQCXIP cFLAG

   Plasmid

   #19138

   Depositor

   Zena Werb

   Article

   Lemieux et al J Biol Chem. 2007 May 18. 282(20):14836-

   Insert

   ADAM10 (Adam10 Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   delta 56-457

   Promoter

   Available since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pVRa22_4450

   Plasmid

   #49676

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF22_4450
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVRa03_1198

   Plasmid

   #49655

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF03_1198
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRS313_ATP3

   Plasmid

   #120253

   Purpose

   Expression of ATP3 in yeast

   Depositor

   Markus Ralser

   Article

   Vowinckel et al Nat Metab. 2021 Nov;3(11):1521-1535. doi

   Insert

   ATP3 (ATP3 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ATP3

   Available since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. ND4 left TALE–G1333 DddAtox C

   Plasmid

   #158092

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   SOD2 MTS–3xHA–ND4 left TALE–G1333 DddAtox-C–1xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. plc242-GFP-ITFG2

   Plasmid

   #87049

   Purpose

   mammalian expression of ITFG2

   Depositor

   David Sabatini

   Article

   Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

   Insert

   ITFG2 (ITFG2 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. ND4 right TALE–G1333 DddAtox N

   Plasmid

   #158096

   Purpose

   expresses the right-side ND4 TALE–DddAtox half in mammalian cells

   Depositor

   David Liu

   Article

   Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

   Insert

   COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVRc22_4450

   Plasmid

   #49741

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   AS22_4450
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pLux

   Available since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK5-MYC-ITFG2

    Plasmid

    #87046

    Purpose

    mammalian expression of ITFG2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    ITFG2 (ITFG2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_ACTB-i1 sgRNA / hSpCas9

    Plasmid

    #172825

    Purpose

    Mammalian expression of a sgRNA targeting the intron 1 position 1 of ACTB (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    sgRNA targeting the intron 1 of ACTB under the U6 promotor and hSpCas9 under the CAG promotor
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-sgAtm/Cre

    Plasmid

    #89643

    Purpose

    Expresses an Atm-targeting gRNA and Cre-recombinase

    Depositor

    Monte Winslow

    Article

    Rogers et al Nat Methods. 2017 May 22. doi: 10.1038/n

    Insert

    gRNA targeting Atm (Atm Mouse)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. G1333 DddAtox-C–SaKKH-Cas9(D10A)

    Plasmid

    #157838

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV-10xMS2 oligo-library based

    Plasmid

    #158200

    Purpose

    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.

    Depositor

    Roee Amit

    Article

    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

    Insert

    10 binding sites of MS2 from oligo library experiment
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PAX6 sgRNA7

    Plasmid

    #68466

    Purpose

    targeting PAX6 gene

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Insert

    sgRNA targeting PAX6 (PAX6 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. TTR C10S S85C K80D

    Plasmid

    #184743

    Purpose

    bacterial expression of human TTR mutants

    Depositor

    Peter Wright

    Article

    Ferguson et al Biochemistry. 2021 Mar 16;60(10):756-764

    Insert

    TTR (TTR Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C10S, S85C, K80D

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. KG371-GFP-mios

    Plasmid

    #87052

    Purpose

    mammalian expression of mios

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    mios (MIOS Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G1397 DddAtox-N–SaKKH-Cas9(D10A)

    Plasmid

    #157839

    Purpose

    expresses split DddAtox-Cas9 construct in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

    Insert

    G1397 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330 _HITI donor B6

    Plasmid

    #172847

    Purpose

    DRS-2 sgRNA expression under a U6 promotor and HITI donor B6 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-2 sgRNA (with SpCas9).

    Depositor

    Haining Zhong

    Article

    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

    Insert

    DRS-2 sgRNA and HITI donor (phase 1) encoding mEGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOPS0380

    Plasmid

    #133230

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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