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mKO2-Cx43-7

Plasmid

#57867

Purpose

Localization: Gap Junctions, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pAF1-VP16-SeqFosC

Plasmid

#51432

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE C)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosC

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16-SeqFosD

Plasmid

#51433

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE D)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosD

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-SeqFosC

Plasmid

#51428

Purpose

Artificial transcription factor with no activator domain targeting c-Fos (TALE C)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosC

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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pAF1-VP16-SeqFosA

Plasmid

#51429

Purpose

Artificial transcription factor with VP16 targeting c-Fos (TALE A)

Depositor

Xavier Darzacq

Article

Senecal et al Cell Rep. 2014 Jun 25. pii: S2211-1247(1

Insert

SeqFosA

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Academic Institutions and Nonprofits only

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p416GAL1-Skn7t-L-IFP-F[1] (Yeast)

Plasmid

#52896

Purpose

Skn7t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

Skn7t-L-IFP-F[1]

Tags

L-IFP-F[1]

Expression

Yeast

Promoter

GAL1

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO-sh-hSH3BP4

Plasmid

#46895

Purpose

expression of shRNA against human SH3BP4

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (SH3BP4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
UAS-Myc-dASUN

Plasmid

#47036

Purpose

Drosophila expression of tagged dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Tags

Myc

Expression

Insect

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
TV3-mCHY-dASUN

Plasmid

#47034

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Tags

mCHY

Expression

Insect

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
TV3-eGFP-dASUN

Plasmid

#47033

Purpose

Drosophila testes specific expression of fluorescent dASUN

Depositor

Laura Lee

Article

Anderson et al Mol Biol Cell. 2009 Jun;20(11):2709-21.

Insert

Asunder (asun Fly)

Tags

eGFP

Expression

Insect

Available Since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only
pLKO-sh-mSH3BP4

Plasmid

#46894

Purpose

expression of shRNA against mouse SH3BP4

Depositor

Do-Hyung Kim

Article

Kim et al Mol Cell. 2012 Jun 29;46(6):833-46. doi:

Insert

SH3BP4 (Sh3bp4 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

W92A (mutated SH3 domain)

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (S204A)

Plasmid

#17699

Depositor

Xinmin Zheng

Article

Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

Insert

PTPalpha S204A (PTPRA Human)

Expression

Mammalian

Mutation

S204A

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pFSW Doc2alpha Halotag

Plasmid

#128817

Purpose

To make virus expressing Doc2alpha Halotag

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2alpha (Doc2a Rat)

Use

Lentiviral

Tags

HaloTag-IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Doc2beta Halotag

Plasmid

#128818

Purpose

To make virus expressing Doc2beta Halotag

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Use

Lentiviral

Tags

HaloTag-IRES-EGFP

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-12xSunTag(SRv1.2-2P)

Plasmid

#185802

Purpose

one plasmid encoding scFv GFP and pcp-10xSunTag components for SunRISER SRv1.2

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-12xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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LUC05 (L/Cys/P)

Plasmid

#112146

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Mehta et al J Biol Chem. 2004 Sep 3;279(36):37852-9.

Insert

Luciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)

Expression

Mammalian

Mutation

none

Available Since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mULK1M92A

Plasmid

#31964

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (Ulk1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

M92A

Promoter

CMV, SP6

Available Since

Oct. 31, 2011

Availability

Academic Institutions and Nonprofits only
GFP-(SIM)1

Plasmid

#126954

Purpose

Bacterial expression plasmid for eGFP-(SIM)1 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Tags

eGFP

Expression

Bacterial

Promoter

unknown

Available Since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 1-1290

Plasmid

#78579

Purpose

Expression flag-USP19 1-1290 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 1-1290 (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 1291 residue to stop condon

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pTrans_9xD3B_Cluc_Norm

Plasmid

#68415

Purpose

CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

Cypridina Luciferase-2A-Venus YFP

Use

Luciferase

Expression

Mammalian

Mutation

YFP: M153T,V163A,S175G ("Venus") YFP Va…

Promoter

minimalCMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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