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We narrowed to 4,785 results for: pcr

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Showing: 881 - 900 of 4785 results

  5. pJH131

    Plasmid
    #48260
    Purpose
    Template plasmid for PCR-based transplant of Saccharomyces cerevisiae GAL10/GAL1 bidirectional promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    GAL10/GAL1 bidirectional promoter
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    GAL10/GAL1 bidirectional
    Available Since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    48260-plasmid-map-sequence-id-69695

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  6. IpO

    Plasmid
    #48233
    Purpose
    Base vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    URA3 promoter and none
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48233-plasmid-map-sequence-id-69537

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  13. pJH124

    Plasmid
    #48259
    Purpose
    Template plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.
    Depositor
    Ronald Davis
    Article
    Horecka et al Yeast. 2014 May;31(5):185-93. doi: 10.10
    Inserts
    URA3 3' fragment
    URA3 5' fragment
    Ag TEF1 promoter
    Use
    Routine cloning vector
    Mutation
    -242 upstream to URA3 ORF +495 and URA3 ORF from …
    Promoter
    Ag TEF1 promoter
    Available Since
    Feb. 10, 2014
    Availability
    Academic Institutions and Nonprofits only
    48259-plasmid-map-sequence-id-69693

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  15. pOpen-ETSSB

    Plasmid
    #165549
    Purpose
    ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Extreme Thermostable Single-Stranded DNA Binding Protein
    Use
    Synthetic Biology
    Available Since
    Aug. 10, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165549-plasmid-map-sequence-id-339430

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  16. pOpen-BovDNTT

    Plasmid
    #165580
    Purpose
    Adds dT at the end of PCR blunted DNA fragments/vectors that can then be used in TOPO cloning; Addition of homopolymer tails to the 3' ends of DNA; Labeling the 3' ends of DNA with modified nucleotides (e.g., ddNTP, DIG-dUTP); TUNEL assay (in situ localization of apoptosis); TdT dependent PCR
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    Bovine DNTT
    Use
    Synthetic Biology
    Available Since
    July 28, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165580-plasmid-map-sequence-id-338558

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  18. pEasyG3_mic

    Plasmid
    #184920
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184920-plasmid-map-sequence-id-368413

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  20. pEasyG2_mic

    Plasmid
    #184916
    Purpose
    Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_mic recombines in vivo with a PCR product from pEasyG2_zeo/nat/hph.
    Depositor
    Jeferson Gross
    Article
    Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-
    Insert
    gRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    Oct. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    184916-plasmid-map-sequence-id-371408

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Showing: 881 - 900 of 4785 results