We narrowed to 16,597 results for: puromycin

1. pLCHKOv3-mClover3

   Plasmid

   #209034

   Purpose

   Lentiviral backbone for expressing U6 driven hybrid guide (hg)RNAs with BveI cloning sites, mClover3 and puromycin selection marker

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

   Plasmid

   #208399

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

   Plasmid

   #208398

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   E2-Crimson

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

   Plasmid

   #208400

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ1373_mU6_sgRNA targeting e1

   Plasmid

   #190684

   Purpose

   sgRNA targeting enhancer 1 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 1 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. double_sgRNA targeting e1 and e4

   Plasmid

   #190688

   Purpose

   sgRNAs targeting enhancer 1 and 4 of MYC separately

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNAs targeting enhancer 1 and 4 of MYC separately
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSLQ5004_hU6_sgRNA targeting e7

   Plasmid

   #190687

   Purpose

   sgRNA targeting enhancer 7 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 7 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ5004_hU6_sgRNA targeting e4

   Plasmid

   #190686

   Purpose

   sgRNA targeting enhancer 4 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 4 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ1373_mU6_sgRNA targeting e3

   Plasmid

   #190685

   Purpose

   sgRNA targeting enhancer 3 of MYC

   Depositor

   Stanley Qi

   Article

   Lin et al Science. 2022 Aug 11:eabk3512. doi: 10.1

   Insert

   sgRNA targeting enhancer 3 of MYC
   

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   BFP and Puromycin

   Expression

   Mammalian

   Promoter

   mouse U6 promoter

   Available Since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJET-61-SFFV-T2A-PuroR-polyA-R11a

    Plasmid

    #179892

    Purpose

    Donor with homologous arms for Rab11a to knock in SFFV-T2A-Puromycin resistance-PolyA cassette (for knock out

    Depositor

    Patrick Caswell

    Article

    Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

    Insert

    RAB11A, member RAS oncogene family (RAB11A Human)
    

    Use

    CRISPR; Donor for homologous based knock in

    Expression

    Bacterial and Mammalian

    Promoter

    SFFV

    Available Since

    May 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro

    Plasmid

    #177145

    Purpose

    Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiGuide-PolB1-puro

    Plasmid

    #177146

    Purpose

    Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolBgRNA1 (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-Flag-PolB(T304I)-Puro

    Plasmid

    #177143

    Purpose

    Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiGuide-Puro-NCOA7g3 (BB24)

    Plasmid

    #139459

    Purpose

    Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1a / U6

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV-gRNA-Bcan-Ntrk1_4

    Plasmid

    #136413

    Purpose

    Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV-gRNA-Myb-Qk_1

    Plasmid

    #136415

    Purpose

    Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

    Depositor

    Massimo Squatrito

    Article

    Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

    Insert

    U6_sgRNA(Myb)_U6_sgRNA(Qk)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid

    #130921

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    TIRAP-ΔNcGAS
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the N ter…

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-Fyn-cGASΔN-HA

    Plasmid

    #130922

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the Fyn dual acylation motif (aa1-16); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    Fyn-ΔNcGAS
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the dual …

    Promoter

    CMV

    Available Since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTBL405 CHYRON1 integration construct

    Plasmid

    #126442

    Purpose

    To integrate the CHYRON1 locus at HEK293site3.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON1 hgRNA
    pCMV-puro
    

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDF1-ADAMTS18 (P1035S)

    Plasmid

    #29558

    Depositor

    Yardena Samuels

    Article

    Wei et al Mol Cancer Res. 2010 Nov . 8(11):1513-25

    Insert

    ADAMTS18 (ADAMTS18 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed proline 1035 to serine

    Available Since

    Sept. 7, 2011

    Availability

    Academic Institutions and Nonprofits only