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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,144 results for: LEA

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Showing: 9061 - 9080 of 16144 results

  1. pCRII-Topo Couptf1

    Plasmid
    #45870
    Depositor
    Jeffrey Macklis
    Insert
    Couptf1 in situ probe (Nr2f1 Mouse)
    Use
    In situ
    Mutation
    contains bp# 1755-2381 of NM_010151.2
    Available Since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  3. pJK237

    Plasmid
    #73339
    Purpose
    Produces Acetobacter aceti 1023 citrate synthase with cleavable N-terminal His6/CBD/Ssp-intein fusion
    Depositor
    T. J. Kappock
    Insert
    citrate synthase
    Tags
    His6, Ssp intein, and chitin-binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    73339-plasmid-map-sequence-id-138207

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  4. CS2-H2B-EGFP

    Plasmid
    #132414
    Purpose
    To make synthetic mRNA encoding a nuclear EGFP
    Depositor
    David Kimelman
    Insert
    H2B-EGFP (H2BC21 Synthetic)
    Use
    Synthetic mrna production
    Promoter
    SP6
    Available Since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    132414-plasmid-map-sequence-id-257700

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  5. pCRII-Topo Hnrp

    Plasmid
    #45872
    Depositor
    Jeffrey Macklis
    Insert
    Hnrnpa2b1 in situ probe (Hnrpa2b1 Mouse)
    Use
    In situ
    Mutation
    contains bp# 1145-1370 of NM_182650.3
    Available Since
    July 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  7. MPP7

    Plasmid
    #28149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    membrane protein, palmitoylated 7 (MPP7 Human)
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    PDZ Domain (residues 135-225)
    Available Since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. pMB.MBP.JARID2

    Plasmid
    #247018
    Purpose
    Expression of MBP-JARID2 in insect cells, MBP tags are C3-cleavable (PreScission Protease)
    Depositor
    Chen Davidovich
    Insert
    JARID2 (JARID2 Human)
    Tags
    MBP
    Expression
    Insect
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    247018-plasmid-map-sequence-id-493374

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  9. CPH3737_GST-TEV-Spt6p-tSH2-1223-1453

    Plasmid
    #227087
    Purpose
    Bacterial expression of K1355A/K1435A double-mutant GST-tagged Spt6 tSH2 domain (1223-1453)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    K1355A, K1435A
    Promoter
    T7
    Available Since
    April 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    227087-plasmid-map-sequence-id-470399

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  10. pET-Impact-T4gp32(L301G)

    Plasmid
    #228209
    Purpose
    Expression in BL21(DE3) of single strand DNA binding protein L301G
    Depositor
    Stephen Benkovic
    Insert
    61
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated leucine 301 to glycine (L301G), improves …
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228209-plasmid-map-sequence-id-453946

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  13. CPH3747_GST-TEV-Spt6p-tSH2-1223-1452

    Plasmid
    #227086
    Purpose
    Bacterial expression of R1282H single-mutant GST-tagged Spt6 tSH2 domain (1223-1452)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227086-plasmid-map-sequence-id-451101

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  14. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  15. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  16. pFastBac HTB His-TEV-BN-rNLRP1(S969A)-FLAG

    Plasmid
    #217324
    Purpose
    Purification of autocleavage-deficient Brown Norway rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217324-plasmid-map-sequence-id-438435

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  17. pFastBac HTB His-TEV-LEW-rNLRP1(S969A)-FLAG

    Plasmid
    #217321
    Purpose
    Purification of autocleavage-deficient Lewis rat NLRP1 fusion with C-terminal FLAG tag from insect cells
    Depositor
    Yifan Wang
    Insert
    NLRP1
    Tags
    FLAG and His-TEV
    Expression
    Insect
    Mutation
    S969A
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    217321-plasmid-map-sequence-id-433443

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  18. pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q

    Plasmid
    #147264
    Purpose
    Insect Expression of DmNot3-mut2-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-mut2-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147264-plasmid-map-sequence-id-289711

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Showing: 9061 - 9080 of 16144 results