We narrowed to 20,498 results for: pET

1. pLKO.1 RagA shRNA #1

   Plasmid

   #30319

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagA shRNA (RRAGA Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pGEM-sgMUC1_dual-MTS-3x-Set I

   Plasmid

   #162763

   Purpose

   Separately expressing 3 unique sgMUC1_dual-MTS (SetI)

   Depositor

   Antony K Chen

   Article

   Mao et al Nucleic Acids Res. 2019 Nov 18;47(20):e1

   Insert

   sgMUC1_dual-MTS_1; sgMUC1_dual-MTS_2; sgMUC1_dual-MTS_3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pK-myc-Par6C

   Plasmid

   #15474

   Depositor

   Ian Macara

   Article

   Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

   Insert

   Partitioning-defective protein 6C (PARD6A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2007

   Availability

   Academic Institutions and Nonprofits only

4. P628_SpCas9-RecA

   Plasmid

   #87263

   Purpose

   Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

   Depositor

   Yonglun Luo

   Article

   Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

   Insert

   RecA
   

   Use

   CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   no

   Promoter

   CBh

   Available since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO.1 RagA shRNA #2

   Plasmid

   #30320

   Depositor

   David Sabatini

   Article

   Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

   Insert

   RagA shRNA (RRAGA Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 2, 2011

   Availability

   Academic Institutions and Nonprofits only

7. MSP1594

   Plasmid

   #65775

   Purpose

   Human expression vector for S. thermophilus 1 Cas9: CAG-humanSt1Cas9-NLS

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Marathon-RT tetramutant for Split-PE (pEH101)

   Plasmid

   #190109

   Purpose

   Expresses Marathon-RT tetramutant (D14R-D74R-N116K-N197R)

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

   Insert

   Marathon-RT
   

   Use

   Tags

   bpNLS

   Expression

   Mammalian

   Mutation

   D14R, D74R, N116K, N197R

   Promoter

   CMV

   Available since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. dABE

   Plasmid

   #124447

   Purpose

   Expresses dead Cas9 (dCas9)-ABE7.10

   Depositor

   George Church

   Article

   Smith et al Nucleic Acids Res. 2020 May 21;48(9):518

   Insert

   dABE7.10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCW-GFP-CtIP-T847E

    Plasmid

    #71111

    Purpose

    mammalian expession of GFP-CtlP T847E mutation

    Depositor

    Daniel Durocher

    Article

    Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

    Insert

    CtlP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    T847E

    Promoter

    TRE promoter, Tet ON

    Available since

    Dec. 9, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. MUNC18-2

    Plasmid

    #186687

    Purpose

    Expresses MUNC18-2 in insect cells

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    MUNC18-2 (Stxbp2 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Flag pLJM1 RagD 77L

    Plasmid

    #19317

    Depositor

    David Sabatini

    Article

    Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

    Insert

    Ras-related GTP binding D (RRAGD Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S77L

    Promoter

    Available since

    Sept. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. plc242-GFP-Nprl2

    Plasmid

    #87051

    Purpose

    mammalian expression of Nprl2

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJL1 BSMT1

    Plasmid

    #106287

    Purpose

    Expresses BSMT1 in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    BSMT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. mApple-ERM

    Plasmid

    #217377

    Purpose

    ER marker

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCLX-M38s/TOP-dGFP

    Plasmid

    #114295

    Purpose

    Expression of destabilized GFP under the control of the M38-TOP canonical Wnt signaling responsive promoter

    Depositor

    Patrick Salmon

    Article

    Boitard et al Cell Rep. 2015 Mar 3;10(8):1349-61. doi:

    Insert

    dGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    destabilized GFP

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. SNAP-23

    Plasmid

    #186686

    Purpose

    Expresses SNAP-23 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    SNAP23
    

    Use

    Tags

    GST and thrombin site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCaUra3-CUbo-AID*-GFP

    Plasmid

    #212775

    Purpose

    C-terminal PCR-based tagging with CUbo-AID*-yeGFP in budding yeast with CaUra3 selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS-Cre

    Plasmid

    #47117

    Purpose

    Can be used to in vitro transcribe Cre recombinase.

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Insert

    Cre
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Lac

    Available since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pconst #5-RFP

    Plasmid

    #81079

    Purpose

    constitutive promoter expressing RFP, biobrick construction

    Depositor

    Jay Keasling, Paul Adams

    Article

    Dahl et al Nat Biotechnol. 2013 Nov;31(11):1039-46.

    Insert

    RFP
    

    Use

    P15a origin, constitutive promoter, kanr

    Tags

    Expression

    Mutation

    Promoter

    Constitutive Promoter - BBa_J23110

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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