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pD(Nt)_His6-FLAG3-TEV

Plasmid

#223875

Purpose

4G-cloning donor for N-terminal His6-FLAG3-TEV tag

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Insert

His6-FLAG3-TEV

Use

Golden-gate donor

Available since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pLPC53

Plasmid

#209962

Purpose

Contains Level 0 Part: 5' Connector (5C1CSN with double terminator Tcat+sai) for the construction of Level 1 plasmids

Depositor

Thorsten Mascher

Publication

Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

Insert

5' Connector (5C1CSN_Tcat+sai)

Expression

Bacterial

Available since

Sept. 13, 2024

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pSpy

Plasmid

#220184

Purpose

sgRNA expression vector - promoterless backbone

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

promoterless backbone

Use

CRISPR

Expression

Bacterial

Promoter

promoterless

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyA

Plasmid

#220185

Purpose

sgRNA expression vector - pEM7 promoter

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pEM7 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pEM7

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyC

Plasmid

#220187

Purpose

sgRNA expression vector - pBG42 promoter

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG42 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG42

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pSpyD

Plasmid

#220188

Purpose

sgRNA expression vector - pBG28 promoter

Depositor

Larry Halverson

Publication

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

pBG28 promoter

Use

CRISPR

Expression

Bacterial

Promoter

pBG28

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Publication

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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AA271

Plasmid

#216074

Purpose

Fragmid fragment: (CROPseq) guide expression; UMI-compatible

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v5; trRNA_v6 [Sp]; 3'LTR; bGHpolyA_v1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA303

Plasmid

#216071

Purpose

Fragmid fragment: (guide cassette) guide expression

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

U6_v1; BsmBI_v0; BsmBI_v6; trRNA_v1 [Nme2]; terminator_v1

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA054

Plasmid

#215932

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v3}

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA269

Plasmid

#216073

Purpose

Fragmid fragment: (CROPseq) WPRE; 3'LTR; guide expression bGHpolyA

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

WPRE_v1; U6_v1; BsmBI_v0; BsmBI_v1; trRNA_v4 [Sp]; 3'LTR; bGHpolyA_v1

Use

Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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AA102

Plasmid

#215935

Purpose

Fragmid fragment: (guide cassette) guide expression; reverse orientation; enables iBar UMIs

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

rev{U6_v1; DR_v0 [EnAs]; BsmBI_v2; BsmBI_v5}

Use

CRISPR; Fragment

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pRDB_131

Plasmid

#216095

Purpose

Cas12a [EnAs] CRISPRa targeting CD97, CD4, CD26, CD274, positive control

Depositor

John Doench

Publication

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD97, CD4, CD26, CD274 guides

Use

CRISPR and Lentiviral; Positive controls

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnk2a

Plasmid

#208983

Purpose

Riboprobe generation

Depositor

GuangJun Zhang

Publication

Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

Insert

kcnk2a (kcnk2a Zebrafish)

Use

Cloning

Promoter

T7

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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(R)pADH2(D)_P1(SBE214)

Plasmid

#195015

Purpose

promoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1

Depositor

Petri-Jaan Lahtvee

Publication

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

(B) pADH2 (R)

Expression

Bacterial

Available since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_EF-act1_full

Plasmid

#202172

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 full terminator

Use

Synthetic Biology

Mutation

None

Available since

Sept. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_EF-act1_tr

Plasmid

#202171

Purpose

Level 0 plasmid containing a terminator sequence from gene 29812 from Phaeodactylum tricornutum with EF overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

act1 truncated terminator

Use

Synthetic Biology

Mutation

None

Available since

Aug. 29, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-PT2A

Plasmid

#202138

Purpose

Level 0 plasmid containing a dual 2A (P2A and T2A) peptide sequence with CD overhangs used to build level 1 constructs

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Dual 2A peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-mito_sig_pep

Plasmid

#202134

Purpose

Level 0 plasmid containing a kozak consensus sequence and a mitochondria signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Mitochondria signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_BC-sec_sig_pep

Plasmid

#202133

Purpose

Level 0 plasmid containing a kozak consensus sequence and a secretion signal peptide sequence with BC overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Publication

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Secretion signal peptide

Use

Synthetic Biology

Mutation

None

Available since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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