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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,604 results for: LEA

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Showing: 9261 - 9280 of 16604 results

  1. CRF-CapC

    Plasmid
    #251440
    Purpose
    Expresses full length corticotropin releasing factor peptide caged by chemically activated protein domains in mammalian cells
    Depositor
    Wenjing Wang
    Insert
    CRF-CapC
    Use
    AAV
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    251440-plasmid-map-sequence-id-501616

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  2. Protein2PAM_NmeN7A.1

    Plasmid
    #250228
    Purpose
    Express Protein2PAM_NmeN7A.1 nuclease in human cells using a CMV promoter
    Depositor
    Ali Madani
    Insert
    NmeN7A.1
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Nme1Cas9 G956A/D957G/V979I/V980I/Q981A/G982Y/V100…
    Promoter
    CMV
    Available Since
    March 4, 2026
    Availability
    Academic Institutions and Nonprofits only
    250228-plasmid-map-sequence-id-501772

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  3. CPH3949_HIS-MBP-TEV-Tom1p-HECT-2850-3268

    Plasmid
    #227089
    Purpose
    Bacterial expression of HIS-MBP-TEV tagged Tom1p HECT domain (2850-3268)
    Depositor
    Chris Hill
    Insert
    tom1p-HECT (TOM1 Budding Yeast)
    Tags
    HIS-MBP-TEV_cleavage_site
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227089-plasmid-map-sequence-id-451122

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  4. MDH2_EC

    Plasmid
    #224146
    Purpose
    IPTG induces expression of C. elegans protein MDH-2 with a N-terminal His tag.
    Depositor
    Katherine Walstrom
    Insert
    MDH-2 (mdh-2 Nematode)
    Tags
    His tag, Xpress tag, enterokinase cleaveage site
    Expression
    Bacterial
    Available Since
    Sept. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    224146-plasmid-map-sequence-id-444697

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  5. pET24a MDH_CHLP8

    Plasmid
    #204242
    Purpose
    Bacterial expression of C. parvum (green sulfur photosynthetic bacterium) malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDH_CHLP8
    Tags
    6X His
    Expression
    Bacterial
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204242-plasmid-map-sequence-id-413340

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  6. pAAV-MP-84-spCas9-U6-gRNA-tlock

    Plasmid
    #206936
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Promoter
    MP-84
    Available Since
    Sept. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206936-plasmid-map-sequence-id-407723

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  7. pAc5.1B-EGFP-DmNot3-dsRNAres_Q

    Plasmid
    #147260
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147260-plasmid-map-sequence-id-289707

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  8. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Available Since
    Jan. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    137643-plasmid-map-sequence-id-381376

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  9. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Tara Davis
    Insert
    PRPF4 (PRPF4 Human)
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Available Since
    Nov. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    137650-plasmid-map-sequence-id-377729

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  10. pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q

    Plasmid
    #147279
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    147279-plasmid-map-sequence-id-355936

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  11. Z-CARS-8

    Plasmid
    #166555
    Purpose
    scFv of a human scaffold targeting Cysteinyl-tRNA synthetase. Antigen coverage aa 1-748 of 748, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166555-plasmid-map-sequence-id-329263

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  12. Q-JMJD2DA-4

    Plasmid
    #166553
    Purpose
    scFv of a human scaffold targeting Lysine-specific demethylase 4D. Antigen coverage aa 1-342 of 523
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166553-plasmid-map-sequence-id-329243

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  13. T-FAM83BA-6

    Plasmid
    #166551
    Purpose
    scFv of a human scaffold targeting Protein FAM83B. Antigen coverage aa 117-294 of 1011
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166551-plasmid-map-sequence-id-329242

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  14. Q-ALAS2A-11

    Plasmid
    #166549
    Purpose
    scFv of a human scaffold targeting 5-aminolevulinate synthase, erythroid-specific, mitochondria. Antigen coverage aa 143 - 587 of 587
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166549-plasmid-map-sequence-id-329240

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  15. Q-WNK3B-4

    Plasmid
    #166548
    Purpose
    scFv of a human scaffold targeting Serine/threonine-protein kinase WNK3. Antigen coverage aa 123-500 of 1800
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166548-plasmid-map-sequence-id-329239

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  16. L-YARS-18

    Plasmid
    #166534
    Purpose
    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase. Antigen coverage aa 1-528 of 528, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166534-plasmid-map-sequence-id-329206

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  17. L-SACSIN-74

    Plasmid
    #166526
    Purpose
    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166526-plasmid-map-sequence-id-329139

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  18. J-GALTA-3

    Plasmid
    #166523
    Purpose
    scFv of a human scaffold targeting Galactose-1-phosphate uridylyltransferase. Antigen coverage aa 1-379 of 379, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166523-plasmid-map-sequence-id-329136

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  19. A-PRMT3-3

    Plasmid
    #166514
    Purpose
    scFv of a human scaffold targeting Protein arginine methyltransferase 3 isoform 1. Antigen coverage aa 211-531 of 531.
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166514-plasmid-map-sequence-id-329056

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  20. Q-PHIPA-23

    Plasmid
    #166547
    Purpose
    scFv of a human scaffold targeting PH-interacting protein. Antigen coverage aa 1302 - 1434 of 1821, Bromodomain 2
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166547-plasmid-map-sequence-id-328319

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Showing: 9261 - 9280 of 16604 results