167,913 results

1. slc1a3b:myrGCaMP6s-P2A-H2AmCherry

   Plasmid

   #170209

   Purpose

   Drives myrGCaMP6s-P2A-H2AmCherry expression with the slc1a3b promoter in astrocytes. The myrGCaMP6s calcium indicator expressed in the membrane and mCherry labels the nucleus

   Depositor

   Kelly Monk

   Article

   Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

   Insert

   slc1a3b (slc1a3b Zebrafish)
   

   Use

   Zebrafish

   Tags

   myrGCaMP6s-P2A-H2AmCherry

   Promoter

   slc1a3b

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CaNAR2

   Plasmid

   #64728

   Purpose

   FRET-based biosensor for monitoring calcineurin (CaN) phosphatase activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al eLife. 2014 Jul 23;3:e03765. doi: 10.755

   Insert

   CaNAR2
   

   Tags

   Cerulean3 and YPet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-EF1a-PKAsub-mCherry-MinD-IRES-Puro

   Plasmid

   #213118

   Purpose

   Expression of one half of a PKAsub-mCherry-MinD reporter circuit in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   PKAsub-mCherry-MinD (minD Escherichia coli)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SIRT7 Flag

   Plasmid

   #13818

   Purpose

   Mammalian expression of human SIRT7 with flag tag

   Depositor

   Eric Verdin

   Article

   North et al Mol Cell. 2003 Feb . 11(2):437-44.

   Insert

   SIRT7 (SIRT7 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Missing exon 2

   Available Since

   Feb. 19, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-EF1a-MinE-IRES-Blast

   Plasmid

   #213115

   Purpose

   Expression of untagged MinE in mammalian cells

   Depositor

   Scott Coyle

   Article

   Rajasekaran et al Cell. 2023 Dec 27:S0092-8674(23)01339-9.

   Insert

   MinE (minE )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEmerald-Fascin-C-10

   Plasmid

   #54094

   Purpose

   Localization: Actin Bundling Protein, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Insert

   Fascin (FSCN1 Human)
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 16, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. SpCas9-HF1

   Plasmid

   #138556

   Purpose

   Expresses human codon-optimized high-fidelity SpCas9 and blasticidin resistance: EFS promoter-SpCas9-HF1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   SpCas9-HF1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A

   Promoter

   EFS

   Available Since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1-TRE-SHH

   Plasmid

   #134350

   Purpose

   Targeting vector for site specific integration of doxycycline inducible expression of full length human SHH cDNA into human AAVS1 locus

   Depositor

   Lorenz Studer

   Article

   Cederquist et al Nat Biotechnol. 2019 Apr;37(4):436-444.

   Insert

   SHH (SHH Human)
   

   Expression

   Mammalian

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. M43: pHR-SFFVp-iLID::EGFP::FTH1

   Plasmid

   #122149

   Purpose

   Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

   Depositor

   Clifford Brangwynne

   Article

   Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

   Insert

   FTH1 (FTH1 Human)
   

   Use

   Lentiviral

   Tags

   NLS-iLID-GFP

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   March 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-HyPer7-MEM

    Plasmid

    #136465

    Purpose

    Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Tags

    Farnselation tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC-NRP1

    Plasmid

    #158384

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Protoc. 2020 Aug 10. pii: 10.1038/s4

    Insert

    NRP1 (NRP1 Human)
    

    Tags

    MAC-tag

    Expression

    Mammalian

    Available Since

    Aug. 25, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. Spectrin-M-cpstFRET

    Plasmid

    #61109

    Purpose

    Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

    Depositor

    Fred Sachs

    Article

    Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

    Insert

    spectrin-cpstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-NL1(-)

    Plasmid

    #15260

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Neuroligin 1 (Nlgn1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. TetO-FUW-Lhx2

    Plasmid

    #61537

    Purpose

    Expresses Lhx2 in mammalian cells

    Depositor

    Rudolf Jaenisch

    Article

    Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

    Insert

    Lhx2 (Lhx2 Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHDR-USR-SpCas9

    Plasmid

    #208829

    Purpose

    HDR Universal Surrogate Reporter with a sgRNA and consistently expressed SpCas9. The PuroR reporter gene was designed to be repaired as a selecting marker gene only by the sgRNA/Cas9-triggered HDR.

    Depositor

    Zhiying Zhang

    Article

    Yan et al Mol Ther Nucleic Acids. 2020 Mar 6;19:77

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (AAV1)

    Viral Prep

    #105545-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (#105545). In addition to the viral particles, you will also receive purified pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 plasmid DNA. CMV-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.

    Depositor

    James M. Wilson

    Promoter

    HI

    Tags

    EGFP

    Available Since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

17. AAV-FLEX-EGFP-mir30(Scn9a-scrambled)

    Plasmid

    #79671

    Purpose

    Scrambled control for Cre-dependent knockdown of Scn9a (Nav1.7)

    Depositor

    Scott Sternson

    Article

    Branco et al Cell. 2016 Jun 16;165(7):1749-61. doi: 1

    Insert

    EGFP
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHCMV-LCMV-Arm53b

    Plasmid

    #15796

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    LCMVArm53b envelope glycoprotein
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE3G-EBF1

    Plasmid

    #96965

    Purpose

    Dox-inducible expression of Ebf1 in mammalian cells

    Depositor

    Elena Cattaneo

    Article

    Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

    Insert

    Ebf1 (Ebf1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    July 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRIPZ Myc2-mouse TTP WT

    Plasmid

    #107000

    Purpose

    Expresses epitope-tagged wild-type murine TTP

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    tristetraprolin (Zfp36 Mouse)
    

    Tags

    Double Myc peptide epitope tag

    Expression

    Mammalian

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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