178,552 results
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Plasmid#119971PurposeExpresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE proteinDepositorInsertGAG-nlsCRErec
TagsnlsExpressionMammalianPromoterhCMVAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a-CysRS-His
Plasmid#124107PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBLO 62.5
Plasmid#123124PurposeHuman expression vector for planctomyces CasX and sgRNADepositorInsertsCasX protein
CasX sgRNA
UseCRISPRExpressionMammalianAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1_MMP-9-HA
Plasmid#121172PurposeExpression of mouse MMP9DepositorInsertMMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
TagsHAExpressionMammalianMutationSee depositor comments belowPromoterCMV immediate/earlyAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mNeonGreen-2A-mTurquoise2
Plasmid#98885PurposeProduces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRETDepositorInsertmNeonGreen-2A-mTurquoise2
ExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HIV-1 LTR-gfp
Plasmid#115809PurposeHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vprDepositorInsertgfp and ngfr
UseLentiviralPromoterHIV1 LTRAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EFS-GFP
Plasmid#110834Purposepositive control for GFP expressionDepositorInsertelongation factor 1alpha binding sequence (EFS) at GFP promoter region
UseLentiviralExpressionMammalianAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLJM1 EGFP-Tau
Plasmid#108868PurposeExpresses EGFP linked to human TauDepositorInsertTau (MAPT Human)
UseLentiviralTagsEGFPExpressionMammalianMutationhuman four-repeat tau lacking the N-terminal sequ…PromoterCMVAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.tMCK.PI.eGFP.WPRE.bGH
Plasmid#105556PurposeAAV expression of EGFP from tMCK promoterDepositorHas serviceAAV9InsertEGFP
UseAAVExpressionMammalianPromotertMCKAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pZyxin-mMaple3
Plasmid#101151PurposeFluorescent protein for superresolution imaging and a focal adhesion protein for binding substrateDepositorInserthuman codon optimized mMaple3 fused to zyxin
ExpressionMammalianPromotercmvAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-FLEX-Arch-GFP (AAV1)
Viral prep#22222-AAV1PurposeReady-to-use AAV1 particles produced from AAV-FLEX-Arch-GFP (#22222). In addition to the viral particles, you will also receive purified AAV-FLEX-Arch-GFP plasmid DNA. CAG-driven cre-dependent Arch-GFP expression for optogenetic neural inhibition. These AAV preparations are suitable purity for injection into animals.DepositorPublicationPromoterCAGTagsGFP (Cre-dependent)Available sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (AAV Retrograde)
Viral prep#55645-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn Con/Fon hChR2(H134R)-EYFP (#55645). In addition to the viral particles, you will also receive purified pAAV-hSyn Con/Fon hChR2(H134R)-EYFP plasmid DNA. Synapsin-driven, Cre-dependent and Flp-dependent Channelrhodopsin-EYFP expression for optogenetic activation. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsEYFP (Cre- and Flp-dependent)Available sinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40
Plasmid#101064PurposeAAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorHas serviceAAV1InsertCaMPARI2_L398T
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-tTA
Plasmid#99118PurposeAn AAV genome that ubiquitously expresses tTA from the CAG promoterDepositorHas serviceAAV2InserttTA
UseAAVExpressionMammalianMutation*(see below)PromoterCAGAvailable sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CAG-mRuby2
Plasmid#99123PurposeAn AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoterDepositorInsertmRuby2
UseAAVExpressionMammalianPromoterCAGAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
B. subtilis Single Gene Deletion Library--Kanamycin
Plasmid kit#1000000115PurposeA collection of Bacillus subtilis strains in which each non-essential gene has been individually replaced with a kanamycin resistance cassette.DepositorApplicationStrain CollectionsVector typeBacterial ExpressionAvailable sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_22A
Plasmid#91133PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt IIDepositorInsertEngineering Reagent: 35S:AtCas9 + AtU6:gRNA
UseCRISPRExpressionPlantAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEFIRES-P-mCherry-HPos
Plasmid#87159PurposeFluorescent marker of lipid droplets (LDs)DepositorInsertHPos
TagsmCherryExpressionMammalianPromoterEF-1-alphaAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by