164,794 results

1. pLenti_C_Myc_DDK_IRES_Puro_ALDH7A1

   Plasmid

   #106120

   Purpose

   Lentiviral expression of ALDH7A1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   ALDH7A1 (ALDH7A1 Human)
   

   Use

   Lentiviral

   Tags

   DDK (FLAG) and Myc

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLARRYv2-EGFP

   Plasmid

   #233208

   Purpose

   Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP marker

   Depositor

   Alejo Rodriguez-Fraticelli

   Article

   Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-miniDi-P2A-mCherry

   Plasmid

   #204358

   Purpose

   AAV vector for miniDi expression under the control of human synapsin promoter

   Depositor

   Masao Doi

   Article

   Miyake et al Cell Rep Methods. 2024 Oct 21;4(10):1008

   Insert

   miniDi-P2A-mCherry
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mutation

   The third intracellular loop (ICL3) of hM4Di was …

   Promoter

   hSyn

   Available since

   Oct. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR223-PLK2

   Plasmid

   #23691

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   PLK2 (PLK2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP‐RNASEH2B

   Plasmid

   #108697

   Purpose

   For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

   Insert

   RNASEH2B (RNASEH2B Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT3TS-NLS-TurboID

   Plasmid

   #163849

   Purpose

   In vitro transcription of nls-TurboID. Parton lab clone JST

   Depositor

   Rob Parton

   Article

   Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

   Insert

   TurboID
   

   Use

   In vitro transcription of rna

   Tags

   nls

   Expression

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. B-gTEMP/pcDNA3

   Plasmid

   #188447

   Purpose

   B-gTEMP for mammalian expression

   Depositor

   Takeharu Nagai

   Article

   Lu et al Nano Lett. 2022 Jul 27;22(14):5698-5707.

   Insert

   B-gTEMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Use

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAC-SP6

    Plasmid

    #102418

    Purpose

    Ac transposase with SP6 promoter for in vitro transcription

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Insert

    Ac transposase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    SP6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. psiCHECK2-let-7 MT

    Plasmid

    #78261

    Purpose

    psiCHECK2 dual luciferase reporter harboring a mutated let-7 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

    Depositor

    Joanne Weidhaas

    Article

    Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

    Insert

    mutated let-7 target site
    

    Use

    Luciferase; Microrna activity

    Tags

    Expression

    Mammalian

    Mutation

    nucleotides 2-5 and 10-11 changed from CTCC to GA…

    Promoter

    Available since

    June 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-C 16 E7

    Plasmid

    #85035

    Purpose

    C-Flag/HA tagged HPV 16 E7

    Depositor

    Karl Munger

    Article

    Grace et al Virology. 2016 Oct 20;500:71-81. doi: 10

    Insert

    HPV 16 E7 (E7 Human)
    

    Use

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMXs-Oct3/4

    Plasmid

    #13366

    Purpose

    Retroviral expression of mouse Oct3/4

    Depositor

    Shinya Yamanaka

    Article

    Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

    Insert

    POU domain, class 5, transcription factor 1 (Pou5f1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNP1950

    Plasmid

    #222729

    Purpose

    J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bxb1-GT_AIO-TetOn_mNgn2 _Down-Tandem

    Plasmid

    #229795

    Purpose

    Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mNgn2; rtTA-T2A-mTagBFP2 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. CAG-ADAR2-sesRNA-GFP-W3SL

    Plasmid

    #192069

    Purpose

    Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

    Depositor

    Josh Huang

    Article

    Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

    Insert

    sesRNA for mouse Ctip2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCW-HA-hRb-delta-CDK-puro

    Plasmid

    #212673

    Purpose

    Express tagged hRb delta-CDK phosphosite mutant

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    Rb (RB1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    All 15 CDK phospho-sites are mutated to alanines

    Promoter

    TRE

    Available since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid

    #105549

    Purpose

    AAV expression of EGFP from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV5

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJYS1Peftu

    Plasmid

    #85546

    Purpose

    Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    etfu and unknown

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (AAV5)

    Viral Prep

    #84446-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] (#84446). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] plasmid DNA. CAG-driven, Cre-dependent expression of Jaws-KGC-tdTomato-ER2 for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Promoter

    CAG

    Tags

    None

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only