We narrowed to 1,029 results for: gibson assembly

1. pMulti-sgRNA-LacZ

   Plasmid

   #99913

   Purpose

   Receptor plasmid for the assembly of multiple sgRNAs

   Depositor

   Yujie Sun

   Article

   Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pZH512

   Plasmid

   #102666

   Purpose

   Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZH511

   Plasmid

   #102665

   Purpose

   Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHRSV_CHARMS-bDD-5xT6BMs

   Plasmid

   #233939

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 TRAF6 binding motifs

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS-bDD-5xT6BMs
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHRSV_CHARMS-V43D/E52K/R62E

   Plasmid

   #233940

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations in the death domain that disrupt oligomer formation

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS-V43D/E52K/R62E
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHRSV_CHARMS-1x

   Plasmid

   #233923

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS-1x
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHRSV_CHARMS-1xA

   Plasmid

   #233924

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS-1xA
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHRSV_CHARMS-3x

   Plasmid

   #233925

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS-3x
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHRSV_CHARMS-3xA

   Plasmid

   #233926

   Purpose

   Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation

   Depositor

   Marcus Taylor

   Article

   Lichtenstein et al bioRxiv 2024.05.08.593169

   Insert

   CHARMS-3xA
   

   Use

   Lentiviral

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHRSV_CHARMS-5x

    Plasmid

    #233927

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 5 TRAF6 binding motifs

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-5x
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHRSV_CHARMS-5xA

    Plasmid

    #233928

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5xA which contains only 5 TRAF6 binding motifs with a alanine mutation

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-5xA
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pHRSV_CHARMS-S34Y

    Plasmid

    #233929

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-S34Y which contains a mutation in the death domain that disrupt oligomer formation

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-S34Y
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHRSV_CHARMS-11xTA

    Plasmid

    #233932

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 11 TA repeats in place of the death domain

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-11xTA
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMMK805

    Plasmid

    #207139

    Purpose

    Assembled Tn10 with ptac promoter driving gfp

    Depositor

    Nicholas Shikuma

    Article

    Alker et al ISME Commun. 2023 Sep 19;3(1):98. doi: 1

    Insert

    Tn10-Ptac-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBD21_035

    Plasmid

    #109152

    Purpose

    pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of EGFP

    Depositor

    Ron Weiss

    Article

    Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

    Inserts

    mRFP
    EGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV and pTet

    Available since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

    Plasmid

    #196974

    Purpose

    Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

    Depositor

    Lukas Kapitein

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Use

    Tags

    GST and mNeonGreen

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-BbsI-PITCh

    Plasmid

    #127875

    Purpose

    For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

    Depositor

    Peter Kaiser

    Article

    Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAWP78-PVCpnf_pvc12-HiBiT

    Plasmid

    #198308

    Purpose

    Produces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assays

    Depositor

    Feng Zhang

    Article

    Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

    Insert

    PVCpnf_pvc12-HiBiT
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pShoCAST-sgRNA_entry (BO1)

    Plasmid

    #181786

    Purpose

    Expresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge