We narrowed to 1,069 results for: gibson assembly

1. TRE-TDP-43ΔNLS-mClover3

   Plasmid

   #214916

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and C-terminal mClover3. Expression yields cytosolic diffuse TDP-43

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Expression

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Promoter

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMMK ENTR 1 CMV mAG beta-catenin

   Plasmid

   #206258

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mAG Ctnnb1 under the control of CMV promoter.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   mAG Ctnnb1 (Ctnnb1 Mouse)
   

   Use

   Multimate/gateway entr 1

   Tags

   monomeric Azami Green

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET3d-SF-DCTN1-TS

   Plasmid

   #169375

   Purpose

   E. coli expression plasmid for human DCTN1 (longest transcript variant p150glued/isoform 1), with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.

   Depositor

   Bernd Bukau

   Article

   Bertolini et al Science. 2021 Jan 1;371(6524):57-64. doi

   Insert

   dynactin subunit 1 isoform 1 (DCTN1 Human)
   

   Use

   Tags

   6xHis Tag, SplitFlAsH Tag, and TwinStrep Tag

   Expression

   Bacterial

   Mutation

   Point mutations: A139T, I304T, Q322R, T516A, E577…

   Promoter

   Available since

   May 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBD21_037

   Plasmid

   #109154

   Purpose

   pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of iRFP720

   Depositor

   Ron Weiss

   Article

   Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

   Inserts

   mRFP
   iRFP720
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and pTet

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZH509

   Plasmid

   #102664

   Purpose

   Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBD21_033

   Plasmid

   #109150

   Purpose

   pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of Sirius

   Depositor

   Ron Weiss

   Article

   Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

   Inserts

   mRFP
   Sirius
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and pTet

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBD21_036

   Plasmid

   #109153

   Purpose

   pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of mKO2

   Depositor

   Ron Weiss

   Article

   Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

   Inserts

   mRFP
   mKO2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and pTet

   Available since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBD21_034

   Plasmid

   #109151

   Purpose

   pL2 plasmid backbone for assembly of a transcription unit along with constitutive expression of TagBFP

   Depositor

   Ron Weiss

   Article

   Gam et al Nucleic Acids Res. 2019 Oct 10;47(18):e1

   Inserts

   mRFP
   TagBFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV and pTet

   Available since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMulti-sgRNA-LacZ

    Plasmid

    #99913

    Purpose

    Receptor plasmid for the assembly of multiple sgRNAs

    Depositor

    Yujie Sun

    Article

    Shao et al ACS Synth Biol. 2017 Sep 7. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZH512

    Plasmid

    #102666

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZH511

    Plasmid

    #102665

    Purpose

    Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHRSV_CHARMS-2xTIR

    Plasmid

    #233930

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-2xTIR which contain a tandem dimer of TIR domains

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-2xTIR
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNA-Y

    Plasmid

    #238993

    Purpose

    Node A carrying sgRNA-1 that can be used for the assembly of pEND-Y11

    Depositor

    Javier Santos-Moreno

    Article

    Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

    Insert

    sgRNA-1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pHRSV_CHARMS-bDD-5xT6BMs

    Plasmid

    #233939

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 TRAF6 binding motifs

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-bDD-5xT6BMs
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHRSV_CHARMS-V43D/E52K/R62E

    Plasmid

    #233940

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations in the death domain that disrupt oligomer formation

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-V43D/E52K/R62E
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHRSV_CHARMS-1x

    Plasmid

    #233923

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1x which contains only 1 TRAF6 binding motif

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-1x
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHRSV_CHARMS-1xA

    Plasmid

    #233924

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-1xA which contains 1 TRAF6 binding motif with a alanine mutation

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-1xA
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHRSV_CHARMS-3x

    Plasmid

    #233925

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which contains 3 TRAF6 binding motifs

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-3x
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHRSV_CHARMS-3xA

    Plasmid

    #233926

    Purpose

    Mammalian expression of Chimeric Higher-Order Assemblies for Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which contains 3 TRAF6 binding motif with a alanine mutation

    Depositor

    Marcus Taylor

    Article

    Lichtenstein et al bioRxiv 2024.05.08.593169

    Insert

    CHARMS-3xA
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge