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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,049 results for: gibson assembly

Showing: 921 - 940 of 2049 results

  2. pDEST14-SpyCatcher003-TEVs-SpyTag003DA

    Plasmid
    #216283
    Purpose
    Expresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    SpyCatcher003-TEVs-SpyTag003DA
    Tags
    His6 and TEV cleavage site
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216283-plasmid-map-sequence-id-433206

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  4. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

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  6. pFP.R267

    Plasmid
    #138179
    Purpose
    Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(SGY).gp41-1.(SSS)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138179-plasmid-map-sequence-id-273462

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  7. pFP.R377

    Plasmid
    #138247
    Purpose
    Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HDG).Mja-KlbA*.(CSG)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138247-plasmid-map-sequence-id-273495

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  8. p006-GFP-Logic-AND

    Plasmid
    #112237
    Purpose
    GFP from jellyfish Aquorea sp. under D61 AND gate-like ara+lac promoter with AraC repressor; lacI repressor; kanamycin resistant.
    Depositor
    Jaroslaw Bryk
    Article
    Siddall et al bioRxiv 2021.06.20.449138
    Inserts
    GFP under control of doubly-repressible promoter
    GFP
    araC
    Expression
    Bacterial
    Promoter
    AND-gate and pLacI
    Available Since
    Aug. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    112237-plasmid-map-sequence-id-218819

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  10. pFP.R265

    Plasmid
    #138167
    Purpose
    Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(VDA).M86.(SDL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138167-plasmid-map-sequence-id-273521

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  12. pFP.R272

    Plasmid
    #138221
    Purpose
    Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(AGG).SspGyrB.(SAK)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138221-plasmid-map-sequence-id-273511

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  13. pFP.R364

    Plasmid
    #138234
    Purpose
    Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).Cth-Ter*.(SEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138234-plasmid-map-sequence-id-273460

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  16. pFP.R401

    Plasmid
    #138222
    Purpose
    Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(IGC).TerThyX*.(SFD)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138222-plasmid-map-sequence-id-273515

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  17. pFP.R360

    Plasmid
    #138230
    Purpose
    Expresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(HGK).Ama-Ter*.(SEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138230-plasmid-map-sequence-id-273492

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  20. pFP.R371

    Plasmid
    #138241
    Purpose
    Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)
    Depositor
    Baojun Wang
    Article
    Pinto et al Nat Commun. 2020 Mar 23;11(1):1529. doi:
    Inserts
    araC (araC )
    mCherryN(NGK).MP-M-DnaB*.(TEL)mCherryC.H6
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    P(araBAD) and iPC
    Available Since
    March 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    138241-plasmid-map-sequence-id-273486

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Showing: 921 - 940 of 2049 results