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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,322 results for: LEA

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Showing: 9381 - 9400 of 12322 results

  10. GRPR-DuET

    Plasmid
    #213306
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Publication
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    GRPR (GRPR Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213306-plasmid-map-sequence-id-430844

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  11. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Publication
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Available since
    April 18, 2024
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

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  12. pMAT_M4_YVTM_YQNQ

    Plasmid
    #197084
    Purpose
    This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Publication
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M4, which contains the YVTM and YQNQ signaling motifs.
    Expression
    Mammalian
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197084-plasmid-map-sequence-id-386821

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  13. pMAT_M10_PQQAT

    Plasmid
    #197093
    Purpose
    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Publication
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M10, which contains the PQQAT signaling motif.
    Expression
    Mammalian
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197093-plasmid-map-sequence-id-386838

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  14. pMAT_M11_PQEINF

    Plasmid
    #197094
    Purpose
    This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Publication
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M11, which contains the PQEINF signaling motif.
    Expression
    Mammalian
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197094-plasmid-map-sequence-id-386825

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  15. pMAT_M12_RPTAVEG

    Plasmid
    #197095
    Purpose
    This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_CD3zP2AEGFP
    Depositor
    Wendell Lim
    Publication
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    This plasmid contains the coding sequence for signaling peptide M12, which contains the RPTAVEG signaling motif.
    Expression
    Mammalian
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197095-plasmid-map-sequence-id-386819

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  16. pMAT_M13_6xSAG

    Plasmid
    #197096
    Purpose
    This plasmid contains the coding sequence for the control peptide M13, which contains a linker with six repeats of serine-alanine-glycine. Digest this insert and add it to pHR_pSFFV_scFVCD19_CD8a_C
    Depositor
    Wendell Lim
    Publication
    Daniels et al Science. 2022 Dec 16;378(6625):1194-1200
    Insert
    Control peptide M13, which contains a linker with six repeats of serine-alanine-glycine (SAG)
    Expression
    Mammalian
    Available since
    April 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    197096-plasmid-map-sequence-id-386824

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  19. pT7CFE1_EVD68_3ABC_C147R

    Plasmid
    #212978
    Purpose
    Encodes EV-D68 MO/14/18947 3ABC with active site mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 3ABC C147R
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Cysteine 147 in 3C to Arginine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212978-plasmid-map-sequence-id-420358

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  20. pT7CFE1_EVD68_VP12A_N84T

    Plasmid
    #212983
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A with telaprevir resistance mutation optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A N84T
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Mutation
    changed Asparagine 84 from 2A to Threonine
    Promoter
    T7
    Available since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212983-plasmid-map-sequence-id-420352

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Showing: 9381 - 9400 of 12322 results