We narrowed to 62,357 results for: cloning
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Plasmid#242601PurposeA Tol2 expression vector containing the Crestin enhancer driving FUCCI to label cell cycle in neural crest cells followed by an IRES-EGFP-caax to label all neural crest cells green.DepositorInsertmCerulean-zGEM-P2A-mCherry-zCdt1
UseZebrafish expressionPromoterCrestin enhancerAvailable SinceApril 7, 2026AvailabilityAcademic Institutions and Nonprofits only -
pTol2-Ubi-lsl-EGFP-nls-mCherry:fli1aep-CreI-zf1-BFP (JDW 1236)
Plasmid#229816PurposeA Tol2 based expression vector containing an Ubi driven loxP flanked GFP followed by an nls-mCherry reporter with fli1-TagBFP-zCre in the opposite direction.DepositorInsertloxp-EGFP-stop-lox
UseTol2 based expression vectorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2pA-2xIns-fli1ep-zCreI-BFP (JDW 1218)
Plasmid#229819PurposeA gateway compatible Tol2 destination vector containing the fli1a promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)
Plasmid#229831PurposeA gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.DepositorTypeEmpty backboneUseTol2 destination vectorAvailable SinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS6-NUSA-TEV-GG
Plasmid#215798PurposeCloning of target gene with N terminal His6 NUSA tag and TEV cleavageDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CT-sfGFP-HN-GG
Plasmid#215803PurposeCloning of target gene with C terminal TEV cleavable sfGFP HisAsn6 tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC-CTH10STII-GG
Plasmid#215804PurposeCloning of target gene with C terminal TEV cleavage of His10 and Twin Strep tagDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDEST26-NAT1-C-HA
Plasmid#195178PurposeVector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloningDepositorAvailable SinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pePEmax-pPuro
Plasmid#215688PurposeCloning vector for Uni-Vector prime editing systemDepositorInsertPEmax
UseUnspecifiedPromoterEFS promoterAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Trono-EFS-Blast-P2A-TurboRFP
Plasmid#228893PurposeCloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate2-P2A-TurboID-NS
Plasmid#166568PurposeMultisite Gateway middle entry clone encoding TurboID with mKate2 marker. Parton lab clone IYNDepositorInsertmKate2-P2A-TurboID
UseGateway CloningAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pME-TurboID-NS
Plasmid#166566PurposeMultisite Gateway middle entry clone encoding TurboID. Parton lab clone JRPDepositorInsertTurboID
UseGateway CloningAvailable SinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpCas9_sgRNA_expression_in_pBluescript
Plasmid#122089PurposeU6 driven SpCas9 sgRNA expression vector for cloning own guidesDepositorAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM4951 (pCV0063)
Plasmid#120084PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 1 (NS1).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
Available SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM4937 (pCV0049)
Plasmid#120082PurposeCyanobacteria cloning vector. Chromosomal integration into Synechococcus elongatus neutral site 2 (NS2).DepositorInsertPlasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS2, CV-aph1, CV-ccdB-SwaI
UseOtherAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOGG010
Plasmid#113984PurposeVector construction module. RK2 (IncP) low-copy number origin of replication for construction of Golden Gate cloning vectorsDepositorInsertRK2 (IncP)
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC57-4eCOL2A1
Plasmid#97211PurposeFor cloning of a COL2A1-based, chondrogenesis-responsive promoter, and subcloning this insert into new vectorsDepositorInsert4 repeats of COL2A1 intronic enhancer (+2126/+2174) upstream of core promoter (-164/+37) (COL2A1 Human)
UseVector is designed for cloning and generation of …PromoterCOL2A1 regulatory elementsAvailable SinceOct. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Kusabira Orange-N1 (KO)
Plasmid#54793PurposeLocalization: N1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneTagsKusabira OrangeExpressionMammalianAvailable SinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPOM034_P7_3"Lys3
Plasmid#216459Purpose3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.DepositorInsert3''lys3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPOM038_P8b_5'Lys3
Plasmid#216463Purpose5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.DepositorInsert5'lys3
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only