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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,958 results for: pET

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Showing: 9521 - 9540 of 9958 results

  6. FnRPR9-10

    Plasmid
    #182568
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant
    Depositor
    Timothy Springer
    Publication
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1374A, P1376A, R1379A
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182568-plasmid-map-sequence-id-357182

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  7. FnRPR8-10

    Plasmid
    #182570
    Purpose
    Human fibronectin fragment, type-III repeats 8-10, R1374A, P1376A, R1379A mutant
    Depositor
    Timothy Springer
    Publication
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 8-10
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1374A, P1376A, R1379A
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182570-plasmid-map-sequence-id-357184

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  8. TDRD3:JMC006-H10:C42283

    Plasmid
    #183981
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    TDRD3 in Complex with anti-TDRD3 FAB (TDRD3 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    May 10, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    183981-plasmid-map-sequence-id-361433

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  10. CMV-10xMS2 no wild type

    Plasmid
    #158204
    Purpose
    Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted form the oligo pool experiment. It does not contain the MS2-wt sequence, therefore QCP cannot bind it.
    Depositor
    Roee Amit
    Publication
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of MS2 without the WT sequence from oligo library experiment
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158204-plasmid-map-sequence-id-311816

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  11. CMV-10xQb no wild type

    Plasmid
    #158205
    Purpose
    Cassette of 10 different binding sites with high affinity to Qb phage coat protein, as extracted form the oligo pool experiment. It does not contain the Qb-wt sequence, therefore MCP cannot bind it.
    Depositor
    Roee Amit
    Publication
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of QB coat protein without the WT sequence based on the oligo library experiment
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158205-plasmid-map-sequence-id-311817

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  15. 6DXH (USP05)

    Plasmid
    #119968
    Purpose
    Bacterial expression for structure determination
    Depositor
    Cheryl Arrowsmith
    Insert
    USP05 (USP5 Human)
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    contains amino acids 171-290
    Promoter
    T7
    Available since
    Jan. 8, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    119968-plasmid-map-sequence-id-232692

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  16. pmSod1-Q42L;Q123A-N’His

    Plasmid
    #117710
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Elizabeth Fisher
    Insert
    Sod1 (Sod1 Mouse)
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L; Q123A
    Promoter
    T7
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    117710-plasmid-map-sequence-id-227524

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  17. pAGH50

    Plasmid
    #107254
    Purpose
    Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.
    Depositor
    Erin O'Shea
    Publication
    Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111
    Insert
    vipp1-gfp-fkbp12
    Use
    Suicide vector for destined for double homologous…
    Tags
    GFP and FKBP12
    Expression
    Bacterial
    Promoter
    native
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107254-plasmid-map-sequence-id-214328

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  18. 5T1G (CBX1)

    Plasmid
    #101884
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CBX1 (CBX1 Human)
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101884-plasmid-map-sequence-id-194720

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  19. 2QO2 (EPHA3)

    Plasmid
    #101271
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPHA3 (EPHA3 Human)
    Tags
    Hexahistidine tag with Thrombin cleavage
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 2, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101271-plasmid-map-sequence-id-193505

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  20. UBH12 (3DB4)

    Plasmid
    #25171
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ubh12.414.617:B9 (UHRF1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    contains residues 126-285
    Available since
    April 4, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 9521 - 9540 of 9958 results