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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,512 results for: grna

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Showing: 9561 - 9580 of 16512 results

  6. psgRNA Library

    Pooled Library
    #115927
    Purpose
    ~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens.
    Depositor
    David Bikard
    Article
    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:
    Expression
    Bacterial
    Species
    Escherichia coli
    Use
    CRISPR
    Available Since
    June 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  10. pSpCas9(BB)-2A-miRFP670

    Plasmid
    #91854
    Purpose
    Cas9 from S. pyogenes with 2A-miRFP670, and cloning backbone for sgRNA. Modified Zhang Plasmid #62988
    Depositor
    Ralf Kuehn
    Inserts
    hSpCas9-2A-miRFP670
    BB-guide RNA
    Use
    CRISPR
    Tags
    2A-miRFP670 and 3XFLAG
    Expression
    Mammalian
    Promoter
    Cbh and U6
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    91854-plasmid-map-sequence-id-176529

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  11. LT3GEPIR-CDK1shRNA1

    Plasmid
    #220569
    Purpose
    To inducibly knockdown CDK1 expression
    Depositor
    Sean Lee
    Insert
    CDK1 shRNA1 (CDK1 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220569-plasmid-map-sequence-id-437943

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  13. pRDB_248

    Plasmid
    #245335
    Purpose
    Cas12a CRISPRko positive control guide; targets CD63
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD63 (CD63 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245335-plasmid-map-sequence-id-488021

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  14. LT3GEPIR-HDACshRNA

    Plasmid
    #220573
    Purpose
    To inducibly knockdown HDAC3 expression
    Depositor
    Sean Lee
    Insert
    HDAC3 shRNA (HDAC3 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220573-plasmid-map-sequence-id-437932

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  15. pJH2971

    Plasmid
    #100955
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. KANMX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100955-plasmid-map-sequence-id-193248

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  16. pJH2972

    Plasmid
    #100956
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. URA3MX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100956-plasmid-map-sequence-id-222978

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  17. LT3GEPIR-NEK2shRNA1

    Plasmid
    #220540
    Purpose
    To inducibly knockdown NEK2 expression
    Depositor
    Sean Lee
    Insert
    NEK2 shRNA1 (NEK2 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    220540-plasmid-map-sequence-id-438392

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  18. pJH2970

    Plasmid
    #100954
    Purpose
    Cas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX marker
    Depositor
    James Haber
    Article
    Ranjith et al Protocol Exchange (2017)
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Available Since
    Sept. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    100954-plasmid-map-sequence-id-193247

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  19. LT3GEPIR-NEK2shRNA2

    Plasmid
    #220541
    Purpose
    To inducibly knockdown NEK2 expression
    Depositor
    Sean Lee
    Insert
    NEK2 shRNA2 (NEK2 Human)
    Use
    Lentiviral
    Promoter
    TRE3G (TetOP) promoter
    Available Since
    July 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    220541-plasmid-map-sequence-id-438391

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Showing: 9561 - 9580 of 16512 results