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pAGM65893

Plasmid

#153216

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9, Bar selection cassette

Depositor

Sylvestre Marillonnet

Publication

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR2-R2-PBR-L4

Plasmid

#141017

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR2-L3-PBL-R1

Plasmid

#141016

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Publication

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pQLinkHD_EBFP2

Plasmid

#118853

Purpose

fluorescent protein expression with an N-terminal His-tag

Depositor

Marc Birtwistle

Publication

Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

Insert

EBFP2

Tags

His-tag

Expression

Bacterial

Mutation

EBFP1.2-I128V/V150I/ D155V/V224R

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.sfGFP-RepEntry.iPAC

Plasmid

#100893

Purpose

Lentiviral CRISPR-Cas9 Reporter

Depositor

Dirk Heckl

Publication

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Inserts

sfGFP
PAC

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZS2katGp-RBS33-TP901-proD-oxyR

Plasmid

#78215

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pZS1katGp-RBS31-PhiC31-proD-oxyR

Plasmid

#78217

Purpose

synthetic gene circuit

Depositor

Timothy Lu

Publication

Rubens et al Nat Commun. 2016 Jun 3;7:11658. doi: 10.

Insert

synthetic circuit

Available since

May 11, 2017

Availability

Academic Institutions and Nonprofits only

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pNX3a-PK3

Plasmid

#78346

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
kanMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a-PK6

Plasmid

#78355

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
kanMX6

Expression

Yeast

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pJA318

Plasmid

#74489

Purpose

eF1alpha::puroR::mCherry::T2A::eGFP::d4ODC::wPRE has d4ODC degron

Depositor

Andrew Fire

Publication

Arribere et al Nature. 2016 Jun 1;534(7609):719-723. do

Insert

d4ODC

Use

Lentiviral

Expression

Mammalian

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pYTK092

Plasmid

#65199

Purpose

Encodes URA3 5' Homology as a Type 8b part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

URA3 5' Homology

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK086

Plasmid

#65193

Purpose

Encodes URA3 3' Homology as a Type 7 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

URA3 3' Homology

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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LIIbeta R 3-4

Plasmid

#54361

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 3-4

Plasmid

#54360

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 4-5

Plasmid

#54362

Depositor

Martin Parniske

Publication

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDE43-MCKq19

Plasmid

#27286

Depositor

Dirk Schnappinger

Publication

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag19

Use

Gateway Cloning

Mutation

N/A

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

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pDE43-MCKq20

Plasmid

#27334

Depositor

Dirk Schnappinger

Publication

Blumenthal et al PLoS One. 2010 Dec 22;5(12):e15667. doi:

Insert

qtag20

Use

Gateway Cloning

Mutation

N/A

Available since

May 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET-6xHis-GFP(-7)-neg_tag1

Plasmid

#219419

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Publication

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Insert

GFP(-7)-neg_tag1

Tags

DEEEDD and MGHHHHHGG

Expression

Bacterial

Promoter

T7

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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CROP-seq-mTurquoise2-v2-no-scaffold

Plasmid

#228522

Purpose

CROP-seq-puro-v2 with scaffold sequence removed for the insertion of CRISPRmap Opool with guide, scaffold and barcode sequences, using mTurquoise2 as selection marker

Depositor

Jellert Gaublomme

Publication

Gu et al Nat Biotechnol. 2024 Oct 7. doi: 10.1038

Insert

mTurquoise2

Use

Lentiviral

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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