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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,060 results for: cloning

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Showing: 9641 - 9660 of 63060 results

  1. pJEC819

    Plasmid
    #190815
    Purpose
    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.
    Depositor
    James Chappell
    Publication
    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77
    Insert
    dCas9-XTEN-rpoA
    Use
    CRISPR and Synthetic Biology
    Tags
    XTEN (linker), followed by the N-terminal domain …
    Promoter
    SP30
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    190815-plasmid-map-sequence-id-373717

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  2. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  3. pGD283

    Plasmid
    #129733
    Purpose
    Destination plasmid containing the 35S:XVE estradiol inducible driver and the A-G overhangs for GreenGate cloning of an expression casette for estradiole-inducible expression in plants.
    Depositor
    Rüdiger Simon
    Publication
    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d
    Type
    Empty backbone
    Expression
    Plant
    Available since
    Oct. 31, 2019
    Availability
    Academic Institutions and Nonprofits only
    129733-plasmid-map-sequence-id-258677

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  5. pCfB2189

    Plasmid
    #67532
    Purpose
    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Publication
    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.
    Type
    Empty backbone
    Use
    Cre/Lox; Integrative
    Expression
    Yeast
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    67532-plasmid-map-sequence-id-116416

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  6. pMDC32B-OsMIR390-B/c

    Plasmid
    #61467
    Purpose
    Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.
    Depositor
    James Carrington
    Publication
    Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1
    Insert
    OsMIR390-B/c
    Expression
    Plant
    Mutation
    O. sativa MIR390 precursor sequence including a c…
    Promoter
    2x35S
    Available since
    March 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    61467-plasmid-map-sequence-id-100060

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  9. pTpPBR11_fcpNAT_GWB

    Plasmid
    #154032
    Purpose
    Gateway cloning into T. pseudonana episomal vector
    Depositor
    Raffaela Abbriano
    Type
    Empty backbone
    Use
    Diatom cloning
    Available since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154032-plasmid-map-sequence-id-307051

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  10. mCherry-Espin-Like-ARD-C-18

    Plasmid
    #62743
    Purpose
    Localization: Actin Bundling, C1 Cloning Vector, Excitation:587, Emission:610
    Depositor
    Michael Davidson
    Insert
    EspinLikeARD
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. mTagBFP2-Espin-Like-C-18

    Plasmid
    #62784
    Purpose
    Localization: Actin Bundling , C1 Cloning Vector, Excitation:399, Emission:456
    Depositor
    Michael Davidson
    Insert
    EspinLike
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    March 10, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. pMLLKA-J72019

    Plasmid
    #26010
    Depositor
    Christopher Anderson
    Insert
    N terminal Flag tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    May 10, 2012
    Availability
    Academic Institutions and Nonprofits only
    26010-plasmid-map-sequence-id-12198

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  13. pMLLCK-K112507

    Plasmid
    #26174
    Depositor
    Christopher Anderson
    Insert
    C terminal HA tag
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    April 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    26174-plasmid-map-sequence-id-12295

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  14. pMLLCK-K112603

    Plasmid
    #26173
    Depositor
    Christopher Anderson
    Insert
    C terminal Flag tag
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    April 23, 2012
    Availability
    Academic Institutions and Nonprofits only
    26173-plasmid-map-sequence-id-12294

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  15. pMLLKA-J72022

    Plasmid
    #26013
    Depositor
    Christopher Anderson
    Insert
    N terminal myc tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    April 12, 2012
    Availability
    Academic Institutions and Nonprofits only
    26013-plasmid-map-sequence-id-12201

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  16. pMLLCK-J72034

    Plasmid
    #26179
    Depositor
    Christopher Anderson
    Insert
    C terminal T7 tag
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    Aug. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
    26179-plasmid-map-sequence-id-12300

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  17. pMLLKA-J72016

    Plasmid
    #26007
    Depositor
    Christopher Anderson
    Insert
    N terminal 6X His tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    June 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    26007-plasmid-map-sequence-id-12195

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  18. pMLLKA-J72018

    Plasmid
    #26009
    Depositor
    Christopher Anderson
    Insert
    N terminal E tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    June 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    26009-plasmid-map-sequence-id-12197

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  19. pMLLKA-J72020

    Plasmid
    #26011
    Depositor
    Christopher Anderson
    Insert
    N terminal HA tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    June 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    26011-plasmid-map-sequence-id-12199

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  20. pMLLKA-J72021

    Plasmid
    #26012
    Depositor
    Christopher Anderson
    Insert
    N terminal HSV tag with RBS
    Use
    Synthetic Biology; 2ab assembly vector
    Available since
    June 8, 2011
    Availability
    Academic Institutions and Nonprofits only
    26012-plasmid-map-sequence-id-12200

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Showing: 9641 - 9660 of 63060 results