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pEGFP-C1X-Nup93-mut

Plasmid

#87335

Purpose

To express human Nup93 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP93 (NUP93 Human)

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant changes

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Vps34-Flag

Plasmid

#24398

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Vps34 (PIK3C3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only

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Green Pegassos

Plasmid

#163114

Purpose

Expresses biosensors for pyruvate in mammalian cells

Depositor

Takashi Tsuboi

Article

Harada et al Sci Rep. 2020 Nov 11;10(1):19562. doi: 1

Insert

Green Pegassos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pH6HTC_BRD2-Halo

Plasmid

#175336

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

BRD2 (BRD2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pTS106 pHAGE2 CMVtetO2 MCS-3C-SUMOEu-22xGS-GFP

Plasmid

#199359

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOEu-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOEu-EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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CMV::TVA/tRFP

Plasmid

#164486

Purpose

Dual CMV promoter plasmid to express TVA and TurboRFP

Depositor

Hollis Cline

Article

Faulkner et al eNeuro. 2021 Jun 7;8(4):ENEURO.0477-20.2

Inserts

TVA (CD320 Chicken)
TurboRFP

Expression

Mammalian

Promoter

sCMV and sCMV IE94

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Del 1)

Plasmid

#16601

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 1) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-1 (XmnI->PvuII)

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-LiMETER-v2

Plasmid

#113934

Purpose

Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

Depositor

Yubin Zhou

Article

He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

Insert

LOV2 and Stim1 polybasic domain (PB)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pORANGE LOX Cloning template vector

Plasmid

#139651

Purpose

Cloning template for ORANGE-CAKE conditional knock-in constructs

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Type

Empty backbone

Expression

Mammalian

Promoter

U6

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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dCas12j2 VPR

Plasmid

#188518

Purpose

Expresses FLAG tagged dCas12j2 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDP0437

Plasmid

#221127

Purpose

Plasmid for generating integration cassette to C-terminally tag gene of interest with pHluorin::HPHMX

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

pHluorin

Expression

Yeast

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Calnexin-HT-mGold

Plasmid

#183987

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with localization in the ER.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

Calnexin (CANX Human)

Tags

HaloTag, mGold

Expression

Mammalian

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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BFP-Dcp1a

Plasmid

#153973

Purpose

Fluorescent protein fused to mRNA decapping enzyme 1a

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

mRNA decapping enzyme 1a (DCP1A Human)

Tags

mTagBFP-c1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV flag ERR alpha

Plasmid

#10975

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

Insert

ERR alpha (ESRRA Human)

Tags

flag

Expression

Mammalian

Available Since

Nov. 23, 2005

Availability

Academic Institutions and Nonprofits only

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pDEST-V1-ORF

Plasmid

#73635

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 1 (V1).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-V2-ORF

Plasmid

#73636

Purpose

Gateway™ destination vector encoding N-terminal fusion with Venus fluorescent protein fragment 2 (V2).

Depositor

Darren Saunders

Article

Croucher et al Sci Signal. 2016 Jul 12;9(436):ra69. doi

Type

Empty backbone

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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MBP-C5E-6XTUBE

Plasmid

#217628

Purpose

Bacterial expression of MBP tagged 6XTUBE

Depositor

Ethan Lee

Article

Kassel et al Sci Signal. 2023 Feb 7;16(771):eabn8372.

Insert

6XTUBE

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pPUR-mU6-sgRNA-2XPP7

Plasmid

#194504

Purpose

Empty backbone for sgRNA-2XPP7 in CRIPSR FISHer system

Depositor

Chunqing Song

Article

Lyu et al Cell Res. 2022 Nov;32(11):969-981. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-Claspin

Plasmid

#12659

Depositor

Michele Pagano

Article

Peschiaroli et al Mol Cell. 2006 Aug 4. 23(3):319-29.

Insert

Claspin (CLSPN Human)

Tags

Flag

Expression

Mammalian

Available Since

May 16, 2007

Availability

Academic Institutions and Nonprofits only
hSTARR-seq_SCP1 vector_blocking 4

Plasmid

#99319

Purpose

Vector to measure enhancer activity of candidates from a DNA library by STARR-seq.

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Type

Empty backbone

Use

Hstarr-seq screening vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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