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lenti SYN-FLAG-dCas9-VPR

Plasmid

#114196

Purpose

Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SYN

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2

Plasmid

#14129

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 (TSC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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Ultra-Exon1Q145-Myc-A

Plasmid

#110487

Purpose

Lentiviral vector to express mutant N terminal HTT gene with short 3'UTR in mammalian cells (encodes Myc-tagged mutant huntingtin exon1 fragment with 145 repeats of Q)

Depositor

Baoji Xu

Article

Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

Insert

Homo sapiens huntingtin (HTT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

Partial long 3'UTR wild type huntingtin exon…

Promoter

UbC

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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LgBiT-ST

Plasmid

#207633

Purpose

A plasmid encoding LgBiT fused to SpyTag

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

LgBiT-SpyTag

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII(0.3)-mVenus-FKBP-K7GEF

Plasmid

#225828

Purpose

A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

Depositor

Haruo Kasai

Article

Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

Insert

mVenus-FKBP-K7GEF

Use

AAV

Expression

Mammalian

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pGM227

Plasmid

#220182

Purpose

Expresses exogenous wild-type TOMM70A

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

TOMM70A (TOMM70 Human)

Use

Lentiviral

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-DEST-R1R2

Plasmid

#141056

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Type

Empty backbone

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-HaloTag7-CHMP4A

Plasmid

#184895

Purpose

Stable expression of HaloTag7-CHMP4A in mammalian cells

Depositor

Noboru Mizushima

Article

Yim et al FEBS Lett. 2022 Mar 11. doi: 10.1002/187

Insert

CHMP4A (CHMP4A Human)

Tags

HaloTag7

Expression

Mammalian

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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Lentiviral sgRNA human CD19

Plasmid

#155289

Purpose

Lentiviral expression of sgRNA against human CD19

Depositor

Martin Carroll

Insert

CD19 (CD19 Human)

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Article

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Promoter

CAG

Available Since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

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343 pTol2-fli1epDest

Plasmid

#73486

Purpose

Tol2 vector with chimeric endothelial enhancer/promoter upstream of attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pF155 pcDNA3.1(+)SOD1G93A

Plasmid

#26401

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G93A

Available Since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-Emerald-WPRE-UbC-mCherry

Plasmid

#211802

Purpose

Lentiviral vector plasmid expressing Emerald under the spleen focus-forming virus (SFFV) promoter and mCherry under the Ubiquitin C (UbC) promoter

Depositor

Richard Eva, Bart Nieuwenhuis

Inserts

Emerald
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV and UbC

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-10xMS2 oligo-library based

Plasmid

#158200

Purpose

Cassette of 10 different binding sites with high affinity to MS2 phage coat protein, as extracted from the oligo pool experiment.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

10 binding sites of MS2 from oligo library experiment

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV CreERT2 puro

Plasmid

#22776

Purpose

Retroviral expression of tamoxifen-inducible Cre-ERT2

Depositor

Tyler Jacks

Article

Kumar et al Genes Dev. 2009 Dec 1;23(23):2700-4. doi

Insert

CreERT2

Use

Cre/Lox and Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Jan. 21, 2010

Availability

Academic Institutions and Nonprofits only

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pCIBN-mCherry

Plasmid

#103816

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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lenti EF1a-FLAG-dCas9-VPR

Plasmid

#114195

Purpose

Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-hSTING-dCTT

Plasmid

#214164

Purpose

Retroviral transduction of STING

Depositor

Nan Yan

Article

Wu et al J Exp Med. 2019 Apr 1;216(4):867-883. do

Insert

STING (dCTT) (STING1 Human)

Use

Retroviral

Mutation

dCTT

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCas9_GFP

Plasmid

#44719

Purpose

Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Promoter

CAG

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV2 Bxb1-c22

Plasmid

#242874

Purpose

Mammalian expression of hyperactive Bxb1 Integrase mutant (I87L, A369P, E434G)

Depositor

Jesse Owens

Article

Hew et al Nucleic Acids Res. 2024 Jul 2:gkae534. d

Insert

Bxb1 Integrase

Expression

Mammalian

Mutation

I87L, A369P, E434G

Promoter

CMV

Available Since

Sept. 18, 2025

Availability

Academic Institutions and Nonprofits only

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