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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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169,450 results

Showing: 9761 - 9780 of 169450 results

  2. MMP11-bio-His

    Plasmid
    #53408
    Purpose
    Expresses full-length Stromelysin-3 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.
    Depositor
    Gavin Wright
    Article
    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m
    Insert
    MMP11 (MMP11 Human)
    Tags
    His tag, enzymatic biotinylation sequence, and ra…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    53408-plasmid-map-sequence-id-83189

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  8. MICU-APEX2

    Plasmid
    #79057
    Purpose
    A C-terminal APEX2 fusion to the human mitochondrial calcium uptake 1 protein MICU1
    Depositor
    Alice Ting
    Article
    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n
    Insert
    C-terminal APEX2 fused MICU1 (MICU1 Human, Soybean)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Bacterial and Mammalian
    Promoter
    CMV immearly promotor
    Available Since
    Sept. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    79057-plasmid-map-sequence-id-147904

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  9. pSLIK_Hyg_mTurquoise2_Atg4BC74A

    Plasmid
    #161733
    Purpose
    Doxycycline-inducible expression of Atg4B(C74A) fused with mTurquoise2 to inhibit autophagy
    Depositor
    Alec Kimmelman
    Article
    Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:
    Insert
    Atg4B(C74A) (Atg4b Mouse) (Atg4b Mouse)
    Use
    Lentiviral; Destinatioin vector for gateway cloni…
    Tags
    mTurquoise2
    Expression
    Mammalian
    Mutation
    220-222 TGC (Cys) is changed to GCA (Ala)
    Available Since
    Nov. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    161733-plasmid-map-sequence-id-318425

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  11. pAAV-CaMKIIa-hM4D(Gi)-mCherry

    Plasmid
    #50477
    Purpose
    Gi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoter
    Depositor
    Bryan Roth
    Has Service
    AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    hM4D(Gi)-mCherry (CHRM4 Human)
    Use
    AAV
    Tags
    mCherry
    Mutation
    See supplemental documents for DREADD mutations
    Promoter
    CaMKIIa
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    50477-plasmid-map-sequence-id-370683

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  15. pExp-MBP

    Plasmid
    #112568
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MBP fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis-MBP and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112568-plasmid-map-sequence-id-217224

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Showing: 9761 - 9780 of 169450 results