167,945 results

1. pEGFP-VSP4-E228Q

   Plasmid

   #80351

   Purpose

   Expression of GFP-tagged, dominant negative VPS4 mutant

   Depositor

   Wesley Sundquist

   Article

   Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

   Insert

   VPS4-E228Q (VPS4A Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNatA (pACYCduet-naa10-naa15)

   Plasmid

   #72928

   Purpose

   Allows expression of a modified version of the fission yeast NatA complex - chloramphenicol marker

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

   Inserts

   naa10 (naa10 Fission Yeast, Synthetic)
   naa15 (naa15 Fission Yeast)
   

   Expression

   Bacterial

   Mutation

   Codon optomised for E.coli expression

   Promoter

   T7

   Available Since

   Feb. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-GFP-Kir2.1

   Plasmid

   #107184

   Purpose

   mammalian expression of Kir2.1

   Depositor

   Bikash Pattnaik

   Insert

   KCNJ2 (KCNJ2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. RARS_pNIC-Bio3

   Plasmid

   #153055

   Purpose

   Expression construct for producing Avi tagged Arginyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   RARS (RARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule2-pCNF

   Plasmid

   #85495

   Purpose

   Machinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2009 Jun 30;48(25):5953-62

   Insert

   para-cyanophenylalanine Mj synthetase
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32L L65V F108W Q109M D158G I159P

   Promoter

   lpp (constitutive)

   Available Since

   Feb. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. LENTICRISPR-UPP1_sgRNA1

   Plasmid

   #201634

   Purpose

   CRISPR/Cas9-mediated gene knock-out

   Depositor

   Vamsi Mootha, Alexis Jourdain

   Article

   Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

   Insert

   UPP1 (UPP1 Human)
   

   Use

   CRISPR and Lentiviral

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA Raptor

   Plasmid

   #8513

   Depositor

   David Sabatini

   Article

   Kim et al Cell 2002 Jul 26;110(2):163-75.

   Insert

   Raptor (RPTOR Human)
   

   Tags

   ha

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN3-MGA

   Plasmid

   #107715

   Purpose

   Expression plasmid for 3xFLAG-tagged MGA

   Depositor

   Guntram Suske

   Article

   Stielow et al PLoS Genet. 2018 Jan 30;14(1):e1007193.

   Insert

   MGA (MGA Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 3, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET N6his GFP1-9

    Plasmid

    #182240

    Purpose

    Tripartite split-GFP. E. coli vector. Expression of GFP1-9 detector fragment.

    Depositor

    Stéphanie Cabantous

    Article

    Pedelacq et al Methods Mol Biol. 2019;2025:423-437. doi

    Insert

    GFP1-9
    

    Tags

    6HIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. ATG9A-EGFP

    Plasmid

    #210367

    Purpose

    Expresses ATG9A fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-APOBEC1-YTH

    Plasmid

    #131636

    Purpose

    Expresses APOBEC1 fused to the YTH domain

    Depositor

    Kate Meyer

    Article

    Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

    Insert

    APOBEC1 fused to YTH domain (YTHDF2 Human, Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Oct. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSecUAG-Evol2

    Plasmid

    #163148

    Purpose

    Expresses machinery necessary for selenocysteine incorporation at UAG codons

    Depositor

    Dieter Söll

    Article

    Mukai et al Angew Chem Int Ed Engl. 2018 Jun 11;57(2

    Inserts

    allo-tRNA UTu2D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Cysteine desulfurase
    

    Expression

    Bacterial

    Mutation

    Cysteine 32 switched to Selenocysteine (UAG), Cys…

    Promoter

    EM7, araBAD, native As SelD promoter, and native …

    Available Since

    April 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-mgSrtA

    Plasmid

    #125792

    Purpose

    Transfected into cell of interest for membrane expression. The encoded mgSrtA could enable labeling of the interacting cells which is useful for recording cell-cell interactions.

    Depositor

    P. Chen

    Article

    Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

    Insert

    mgSrtA
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. spNW100

    Plasmid

    #173488

    Purpose

    Expresses spNW100 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spNW100
    

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    P1049L- please see depositor comments

    Promoter

    T7

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-PITCh-LMNB1

    Plasmid

    #207770

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLXIN-Luc

    Plasmid

    #60683

    Purpose

    stable expresses luciferase in mammalian cells by retrovirus system

    Depositor

    Alice Wong

    Insert

    Firefly Luciferase
    

    Use

    Retroviral

    Available Since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

18. PGKp-GFP-Empty

    Plasmid

    #174175

    Purpose

    Control lentiviral vector expressing GFP under control of the human PGK promoter.

    Depositor

    Rod Bremner

    Article

    Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Aug. 19, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pPGK-T7/2-CD44v6-10 (fl)

    Plasmid

    #137820

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v6-10 (fl) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    full length

    Promoter

    PGK

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA (AAV5)

    Viral Prep

    #230681-AAV5

    Purpose

    Ready-to-use AAV5 particles produced from AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA (#230681). In addition to the viral particles, you will also receive purified AiP1636 - pAAV-AiE2116m-minBG-iCre-WPRE-BGHpA plasmid DNA. minBG-driven expression of iCre recombinase in specific populations of brain cells. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Allen Institute for Brain Science, Bosiljka Tasic

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Promoter

    minBG

    Available Since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only